TEMPERATURE-ASSISTED ON-COLUMN FOCUSING

A method of comprising: introducing a sample volume into an inlet end of a liquid chromatography column, wherein the liquid chromatography column includes a focusing segment proximal to the inlet end of the liquid chromatography column and a separation segment proximal to an elute outlet of the liqu...

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Hauptverfasser: Horner, Anthony Robert, Wilson, Rachael-Anh Elizabeth, Weber, Stephen, Groskreutz, Stephen Robert
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creator Horner, Anthony Robert
Wilson, Rachael-Anh Elizabeth
Weber, Stephen
Groskreutz, Stephen Robert
description A method of comprising: introducing a sample volume into an inlet end of a liquid chromatography column, wherein the liquid chromatography column includes a focusing segment proximal to the inlet end of the liquid chromatography column and a separation segment proximal to an elute outlet of the liquid chromatography column; maintaining only the focusing segment at a first temperature as the sample is introduced into the focusing segment; and subsequently heating the focusing segment to a second temperature that is higher than the first temperature after the entire sample volume has been introduced into the focusing segment.
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
SEPARATION
TESTING
TRANSPORTING
title TEMPERATURE-ASSISTED ON-COLUMN FOCUSING
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