DEVICE AND METHOD FOR LARGE VOLUME TRANSFECTION
The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is mova...
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| creator | ALTROGGE, Ludger HEINZE, Andreas HERMSMEIER, Sven GLEISSNER, Timo |
| description | The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6). According to the invention each compartment (26, 27) is designed to hold the suspension and comprises at least one port (7, 8, 10, 11) for charging or discharging the suspension, so as to discharge an aliquot of the suspension from the chamber (6) and at the same time charge a further aliquot of the suspension into the chamber (6), wherein the separating element (13) is moved in a second direction opposite to a first direction, and wherein the separating element (13) separates the aliquots from each other. |
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According to the invention each compartment (26, 27) is designed to hold the suspension and comprises at least one port (7, 8, 10, 11) for charging or discharging the suspension, so as to discharge an aliquot of the suspension from the chamber (6) and at the same time charge a further aliquot of the suspension into the chamber (6), wherein the separating element (13) is moved in a second direction opposite to a first direction, and wherein the separating element (13) separates the aliquots from each other.</description><language>eng</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ELECTROTHERAPY ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MAGNETOTHERAPY ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; RADIATION THERAPY ; SPIRITS ; ULTRASOUND THERAPY ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190516&DB=EPODOC&CC=US&NR=2019144844A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190516&DB=EPODOC&CC=US&NR=2019144844A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ALTROGGE, Ludger</creatorcontrib><creatorcontrib>HEINZE, Andreas</creatorcontrib><creatorcontrib>HERMSMEIER, Sven</creatorcontrib><creatorcontrib>GLEISSNER, Timo</creatorcontrib><title>DEVICE AND METHOD FOR LARGE VOLUME TRANSFECTION</title><description>The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6). According to the invention each compartment (26, 27) is designed to hold the suspension and comprises at least one port (7, 8, 10, 11) for charging or discharging the suspension, so as to discharge an aliquot of the suspension from the chamber (6) and at the same time charge a further aliquot of the suspension into the chamber (6), wherein the separating element (13) is moved in a second direction opposite to a first direction, and wherein the separating element (13) separates the aliquots from each other.</description><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ELECTROTHERAPY</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MAGNETOTHERAPY</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>RADIATION THERAPY</subject><subject>SPIRITS</subject><subject>ULTRASOUND THERAPY</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNB3cQ3zdHZVcPRzUfB1DfHwd1Fw8w9S8HEMcndVCPP3CfV1VQgJcvQLdnN1DvH09-NhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfGhwUYGhpaGJiYWJiaOhsbEqQIAh4Im0g</recordid><startdate>20190516</startdate><enddate>20190516</enddate><creator>ALTROGGE, Ludger</creator><creator>HEINZE, Andreas</creator><creator>HERMSMEIER, Sven</creator><creator>GLEISSNER, Timo</creator><scope>EVB</scope></search><sort><creationdate>20190516</creationdate><title>DEVICE AND METHOD FOR LARGE VOLUME TRANSFECTION</title><author>ALTROGGE, Ludger ; HEINZE, Andreas ; HERMSMEIER, Sven ; GLEISSNER, Timo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US2019144844A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2019</creationdate><topic>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ELECTROTHERAPY</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MAGNETOTHERAPY</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>RADIATION THERAPY</topic><topic>SPIRITS</topic><topic>ULTRASOUND THERAPY</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ALTROGGE, Ludger</creatorcontrib><creatorcontrib>HEINZE, Andreas</creatorcontrib><creatorcontrib>HERMSMEIER, Sven</creatorcontrib><creatorcontrib>GLEISSNER, Timo</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ALTROGGE, Ludger</au><au>HEINZE, Andreas</au><au>HERMSMEIER, Sven</au><au>GLEISSNER, Timo</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>DEVICE AND METHOD FOR LARGE VOLUME TRANSFECTION</title><date>2019-05-16</date><risdate>2019</risdate><abstract>The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6). According to the invention each compartment (26, 27) is designed to hold the suspension and comprises at least one port (7, 8, 10, 11) for charging or discharging the suspension, so as to discharge an aliquot of the suspension from the chamber (6) and at the same time charge a further aliquot of the suspension into the chamber (6), wherein the separating element (13) is moved in a second direction opposite to a first direction, and wherein the separating element (13) separates the aliquots from each other.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ELECTROTHERAPY ENZYMOLOGY HUMAN NECESSITIES HYGIENE MAGNETOTHERAPY MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RADIATION THERAPY SPIRITS ULTRASOUND THERAPY VINEGAR WINE |
| title | DEVICE AND METHOD FOR LARGE VOLUME TRANSFECTION |
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