SOLUTION FOR EXTRACTION OF RNA
A solution for extracting substantially pure RNA from a biological sample is disclosed. The solution for extracting RNA from a biological sample containing RNA and at least DNA comprises: (a) phenol in an amount of more than 50% by volume based on the total amount of the solution; (b) a polyol in an...
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| creator | Akiyama, Hideo Nobumasa, Hitoshi YOSHIMOTO, MAKIKO |
| description | A solution for extracting substantially pure RNA from a biological sample is disclosed. The solution for extracting RNA from a biological sample containing RNA and at least DNA comprises:
(a) phenol in an amount of more than 50% by volume based on the total amount of the solution;
(b) a polyol in an amount of 3 to 10% by volume based on the total amount of the solution;
(c) a guanidinium salt at a concentration of 0.5 to 2.0 M based on the total amount of the solution;
(d) a thiocyanate at a concentration of 0.1 to 0.5 M based on the total amount of the solution; and
(e) a buffer for maintaining the pH of the solution at 4 to 6. |
| format | Patent |
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(a) phenol in an amount of more than 50% by volume based on the total amount of the solution;
(b) a polyol in an amount of 3 to 10% by volume based on the total amount of the solution;
(c) a guanidinium salt at a concentration of 0.5 to 2.0 M based on the total amount of the solution;
(d) a thiocyanate at a concentration of 0.1 to 0.5 M based on the total amount of the solution; and
(e) a buffer for maintaining the pH of the solution at 4 to 6.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20181129&DB=EPODOC&CC=US&NR=2018340166A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20181129&DB=EPODOC&CC=US&NR=2018340166A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Akiyama, Hideo</creatorcontrib><creatorcontrib>Nobumasa, Hitoshi</creatorcontrib><creatorcontrib>YOSHIMOTO, MAKIKO</creatorcontrib><title>SOLUTION FOR EXTRACTION OF RNA</title><description>A solution for extracting substantially pure RNA from a biological sample is disclosed. The solution for extracting RNA from a biological sample containing RNA and at least DNA comprises:
(a) phenol in an amount of more than 50% by volume based on the total amount of the solution;
(b) a polyol in an amount of 3 to 10% by volume based on the total amount of the solution;
(c) a guanidinium salt at a concentration of 0.5 to 2.0 M based on the total amount of the solution;
(d) a thiocyanate at a concentration of 0.1 to 0.5 M based on the total amount of the solution; and
(e) a buffer for maintaining the pH of the solution at 4 to 6.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZJAL9vcJDfH091Nw8w9ScI0ICXJ0BnP93RSC_Bx5GFjTEnOKU3mhNDeDsptriLOHbmpBfnxqcUFicmpeakl8aLCRgaGFsYmBoZmZo6ExcaoAQQciUg</recordid><startdate>20181129</startdate><enddate>20181129</enddate><creator>Akiyama, Hideo</creator><creator>Nobumasa, Hitoshi</creator><creator>YOSHIMOTO, MAKIKO</creator><scope>EVB</scope></search><sort><creationdate>20181129</creationdate><title>SOLUTION FOR EXTRACTION OF RNA</title><author>Akiyama, Hideo ; Nobumasa, Hitoshi ; YOSHIMOTO, MAKIKO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US2018340166A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2018</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Akiyama, Hideo</creatorcontrib><creatorcontrib>Nobumasa, Hitoshi</creatorcontrib><creatorcontrib>YOSHIMOTO, MAKIKO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Akiyama, Hideo</au><au>Nobumasa, Hitoshi</au><au>YOSHIMOTO, MAKIKO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>SOLUTION FOR EXTRACTION OF RNA</title><date>2018-11-29</date><risdate>2018</risdate><abstract>A solution for extracting substantially pure RNA from a biological sample is disclosed. The solution for extracting RNA from a biological sample containing RNA and at least DNA comprises:
(a) phenol in an amount of more than 50% by volume based on the total amount of the solution;
(b) a polyol in an amount of 3 to 10% by volume based on the total amount of the solution;
(c) a guanidinium salt at a concentration of 0.5 to 2.0 M based on the total amount of the solution;
(d) a thiocyanate at a concentration of 0.1 to 0.5 M based on the total amount of the solution; and
(e) a buffer for maintaining the pH of the solution at 4 to 6.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | SOLUTION FOR EXTRACTION OF RNA |
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