PREPARATIVE SEPARATION CHROMATOGRAPH SYSTEM

A preparative separation chromatograph system having a column for temporally separating components in a sample and for discharging an eluate fluid with the components, a detector for obtaining an absorbance spectrum of the eluate fluid, and a fraction collector for continuously creating a chromatogr...

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Hauptverfasser: KAMATA, Etsuho, MISHIMA, Kenichi, MIURA, Hiroshi, MITO, Yasuhiro, YANAGISAWA, Toshinobu
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creator KAMATA, Etsuho
MISHIMA, Kenichi
MIURA, Hiroshi
MITO, Yasuhiro
YANAGISAWA, Toshinobu
description A preparative separation chromatograph system having a column for temporally separating components in a sample and for discharging an eluate fluid with the components, a detector for obtaining an absorbance spectrum of the eluate fluid, and a fraction collector for continuously creating a chromatogram. The system further includes: a peak section determiner; a differential value determiner for calculating a differential spectrum value and for determining whether or not the absolute value of the differential spectrum value is equal to or less than a predetermined value; and a fraction collector controller for controlling the fraction collector so as to fractionate the eluate fluid during a period of time for which it is determined that the chromatogram peak of the target component is present and for which it is also determined the absolute value of the differential spectrum value is equal to or less than the predetermined value.
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
SEPARATION
TESTING
TRANSPORTING
title PREPARATIVE SEPARATION CHROMATOGRAPH SYSTEM
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