NITRILASE FROM ARABIS ALPINA, ITS ENCODING GENE, VECTOR, RECOMBINANT BACTERIAL STRAIN AND USES THEREOF
The disclosure provides a nitrilase from Arabis alpina, which belongs to genus Arabis, family brassicaceae. The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells co...
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| creator | FAN Weirong ZHENG Renchao LIU Tianchun ZHANG Qin WENG Jianfeng ZHENG Yuguo HUANG Youming |
| description | The disclosure provides a nitrilase from Arabis alpina, which belongs to genus Arabis, family brassicaceae. The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells containing nitrilase Aa-Nit can kinetically resolve racemic IBSN at 1.2 M with a 42% conversion rate in 15 hr and >99% ee value. The disclosure provides a regio- and stereoselective method for the preparation of (S)-3-cyano-5-methylhexanoic acid. This method provides an atom economical, mild, environmental friendly industrial method to manufacture (S)-3-cyano-5-methylhexanoic acid. |
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The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells containing nitrilase Aa-Nit can kinetically resolve racemic IBSN at 1.2 M with a 42% conversion rate in 15 hr and >99% ee value. The disclosure provides a regio- and stereoselective method for the preparation of (S)-3-cyano-5-methylhexanoic acid. 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The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells containing nitrilase Aa-Nit can kinetically resolve racemic IBSN at 1.2 M with a 42% conversion rate in 15 hr and >99% ee value. The disclosure provides a regio- and stereoselective method for the preparation of (S)-3-cyano-5-methylhexanoic acid. This method provides an atom economical, mild, environmental friendly industrial method to manufacture (S)-3-cyano-5-methylhexanoic acid.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMES</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNysEKgkAQAFAvHaL6h4GuBmqFXcd11AGdjdm1q0ispyjB_p869AGd3uWto0nYK7foCCq1HaBiwQ6wvbJgDOwdkBhbstRQk1AMNzLeagxKxnbFd4mHAo0nZWzBeUUWQCmhd-TAN6Rkq220msbHEnY_N9G-Im-aQ5hfQ1jm8R6e4T30LkvSPDlfsvyE6fG_9QFBiTWG</recordid><startdate>20170302</startdate><enddate>20170302</enddate><creator>FAN Weirong</creator><creator>ZHENG Renchao</creator><creator>LIU Tianchun</creator><creator>ZHANG Qin</creator><creator>WENG Jianfeng</creator><creator>ZHENG Yuguo</creator><creator>HUANG Youming</creator><scope>EVB</scope></search><sort><creationdate>20170302</creationdate><title>NITRILASE FROM ARABIS ALPINA, ITS ENCODING GENE, VECTOR, RECOMBINANT BACTERIAL STRAIN AND USES THEREOF</title><author>FAN Weirong ; ZHENG Renchao ; LIU Tianchun ; ZHANG Qin ; WENG Jianfeng ; ZHENG Yuguo ; HUANG Youming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US2017058274A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMES</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>FAN Weirong</creatorcontrib><creatorcontrib>ZHENG Renchao</creatorcontrib><creatorcontrib>LIU Tianchun</creatorcontrib><creatorcontrib>ZHANG Qin</creatorcontrib><creatorcontrib>WENG Jianfeng</creatorcontrib><creatorcontrib>ZHENG Yuguo</creatorcontrib><creatorcontrib>HUANG Youming</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>FAN Weirong</au><au>ZHENG Renchao</au><au>LIU Tianchun</au><au>ZHANG Qin</au><au>WENG Jianfeng</au><au>ZHENG Yuguo</au><au>HUANG Youming</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>NITRILASE FROM ARABIS ALPINA, ITS ENCODING GENE, VECTOR, RECOMBINANT BACTERIAL STRAIN AND USES THEREOF</title><date>2017-03-02</date><risdate>2017</risdate><abstract>The disclosure provides a nitrilase from Arabis alpina, which belongs to genus Arabis, family brassicaceae. The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells containing nitrilase Aa-Nit can kinetically resolve racemic IBSN at 1.2 M with a 42% conversion rate in 15 hr and >99% ee value. The disclosure provides a regio- and stereoselective method for the preparation of (S)-3-cyano-5-methylhexanoic acid. This method provides an atom economical, mild, environmental friendly industrial method to manufacture (S)-3-cyano-5-methylhexanoic acid.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | NITRILASE FROM ARABIS ALPINA, ITS ENCODING GENE, VECTOR, RECOMBINANT BACTERIAL STRAIN AND USES THEREOF |
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