PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are...
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| creator | Hitchman Tim S Wall Mark A Barton Nelson R O'Donoghue Eileen Lyon Jonathan D |
| description | In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils. |
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| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_US2016326503A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US2016326503A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_US2016326503A13</originalsourceid><addsrcrecordid>eNrjZAgM8PAPBmIfzwDHYNdgHQW_UGcfV09nBUdnT5dgBVc_Z38XTz93hRAPV18FRz8XBV_XEA9_oIybf5CCr6M3SA4kHBoMU8XDwJqWmFOcyguluRmU3VxDnD10Uwvy41OLCxKTU_NSS-JDg40MDM2MjcxMDYwdDY2JUwUAMIIvyg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM</title><source>esp@cenet</source><creator>Hitchman Tim S ; Wall Mark A ; Barton Nelson R ; O'Donoghue Eileen ; Lyon Jonathan D</creator><creatorcontrib>Hitchman Tim S ; Wall Mark A ; Barton Nelson R ; O'Donoghue Eileen ; Lyon Jonathan D</creatorcontrib><description>In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.</description><language>eng</language><subject>ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE ORUNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL ; ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES ; BEER ; BIOCHEMISTRY ; CANDLES ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; DETERGENT COMPOSITIONS ; DETERGENTS ; ENZYMOLOGY ; FATTY ACIDS THEREFROM ; FIRELIGHTERS ; FUELS ; FUELS NOT OTHERWISE PROVIDED FOR ; HUMAN NECESSITIES ; HYGIENE ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; LIQUEFIED PETROLEUM GAS ; LUBRICANTS ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NATURAL GAS ; ORGANIC CHEMISTRY ; PEAT ; PEPTIDES ; PETROLEUM, GAS OR COKE INDUSTRIES ; PHYSICS ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; RECOVERY OF GLYCEROL ; RESIN SOAPS ; SOAP OR SOAP-MAKING ; SPIRITS ; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BYSUBCLASSES C10G, C10K ; TECHNICAL GASES CONTAINING CARBON MONOXIDE ; TESTING ; USE OF SINGLE SUBSTANCES AS DETERGENTS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161110&DB=EPODOC&CC=US&NR=2016326503A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161110&DB=EPODOC&CC=US&NR=2016326503A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Hitchman Tim S</creatorcontrib><creatorcontrib>Wall Mark A</creatorcontrib><creatorcontrib>Barton Nelson R</creatorcontrib><creatorcontrib>O'Donoghue Eileen</creatorcontrib><creatorcontrib>Lyon Jonathan D</creatorcontrib><title>PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM</title><description>In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.</description><subject>ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE ORUNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL</subject><subject>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CANDLES</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>DETERGENT COMPOSITIONS</subject><subject>DETERGENTS</subject><subject>ENZYMOLOGY</subject><subject>FATTY ACIDS THEREFROM</subject><subject>FIRELIGHTERS</subject><subject>FUELS</subject><subject>FUELS NOT OTHERWISE PROVIDED FOR</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>LIQUEFIED PETROLEUM GAS</subject><subject>LUBRICANTS</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NATURAL GAS</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEAT</subject><subject>PEPTIDES</subject><subject>PETROLEUM, GAS OR COKE INDUSTRIES</subject><subject>PHYSICS</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>RECOVERY OF GLYCEROL</subject><subject>RESIN SOAPS</subject><subject>SOAP OR SOAP-MAKING</subject><subject>SPIRITS</subject><subject>SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BYSUBCLASSES C10G, C10K</subject><subject>TECHNICAL GASES CONTAINING CARBON MONOXIDE</subject><subject>TESTING</subject><subject>USE OF SINGLE SUBSTANCES AS DETERGENTS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2016</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZAgM8PAPBmIfzwDHYNdgHQW_UGcfV09nBUdnT5dgBVc_Z38XTz93hRAPV18FRz8XBV_XEA9_oIybf5CCr6M3SA4kHBoMU8XDwJqWmFOcyguluRmU3VxDnD10Uwvy41OLCxKTU_NSS-JDg40MDM2MjcxMDYwdDY2JUwUAMIIvyg</recordid><startdate>20161110</startdate><enddate>20161110</enddate><creator>Hitchman Tim S</creator><creator>Wall Mark A</creator><creator>Barton Nelson R</creator><creator>O'Donoghue Eileen</creator><creator>Lyon Jonathan D</creator><scope>EVB</scope></search><sort><creationdate>20161110</creationdate><title>PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM</title><author>Hitchman Tim S ; Wall Mark A ; Barton Nelson R ; O'Donoghue Eileen ; Lyon Jonathan D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US2016326503A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2016</creationdate><topic>ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE ORUNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL</topic><topic>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CANDLES</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>DETERGENT COMPOSITIONS</topic><topic>DETERGENTS</topic><topic>ENZYMOLOGY</topic><topic>FATTY ACIDS THEREFROM</topic><topic>FIRELIGHTERS</topic><topic>FUELS</topic><topic>FUELS NOT OTHERWISE PROVIDED FOR</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>LIQUEFIED PETROLEUM GAS</topic><topic>LUBRICANTS</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NATURAL GAS</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEAT</topic><topic>PEPTIDES</topic><topic>PETROLEUM, GAS OR COKE INDUSTRIES</topic><topic>PHYSICS</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>RECOVERY OF GLYCEROL</topic><topic>RESIN SOAPS</topic><topic>SOAP OR SOAP-MAKING</topic><topic>SPIRITS</topic><topic>SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BYSUBCLASSES C10G, C10K</topic><topic>TECHNICAL GASES CONTAINING CARBON MONOXIDE</topic><topic>TESTING</topic><topic>USE OF SINGLE SUBSTANCES AS DETERGENTS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Hitchman Tim S</creatorcontrib><creatorcontrib>Wall Mark A</creatorcontrib><creatorcontrib>Barton Nelson R</creatorcontrib><creatorcontrib>O'Donoghue Eileen</creatorcontrib><creatorcontrib>Lyon Jonathan D</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Hitchman Tim S</au><au>Wall Mark A</au><au>Barton Nelson R</au><au>O'Donoghue Eileen</au><au>Lyon Jonathan D</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM</title><date>2016-11-10</date><risdate>2016</risdate><abstract>In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE ORUNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS ENZYMOLOGY FATTY ACIDS THEREFROM FIRELIGHTERS FUELS FUELS NOT OTHERWISE PROVIDED FOR HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LIQUEFIED PETROLEUM GAS LUBRICANTS MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NATURAL GAS ORGANIC CHEMISTRY PEAT PEPTIDES PETROLEUM, GAS OR COKE INDUSTRIES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPIRITS SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BYSUBCLASSES C10G, C10K TECHNICAL GASES CONTAINING CARBON MONOXIDE TESTING USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE |
| title | PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM |
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