METHODS OF PRODUCING ANTIBODIES IN YEAST

METHODS OF PRODUCING ANTIBODIES IN YEAST

The present invention describes a method for producing an antibody in Pichia pastoris, such as by fed-batch fermentation. The method includes the addition of about 2.0-5.0 g/L of hydroxyurea during the fermentation process to sustain a constant cell density and enhance the whole broth titer of the a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: EAGAN BRUCE E, XING ZIZHUO, XU XUANKUO, YOU LI, CAMPBELL GEORGE S, QIAN NAN-XIN, QIAN YUEMING, LI ZHENGJIAN
Format: Patent
Sprache:eng
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Beschreibung
Zusammenfassung:The present invention describes a method for producing an antibody in Pichia pastoris, such as by fed-batch fermentation. The method includes the addition of about 2.0-5.0 g/L of hydroxyurea during the fermentation process to sustain a constant cell density and enhance the whole broth titer of the antibody. The method may also include a strategy of increasing the ethanol concentration to about 18-22 g/L and then maintaining the ethanol level at about 5-17 g/L to stabilize the cell mass and enhance the production rate of the antibody. The method may further include a respiratory quotient control for monitoring the ethanol profile and to improve the quality of the antibody by, for example, eliminating clipping of the heavy chain.