MONOCOTYLEDON TRANSGENIC METHOD FOR INVADING GROWING POINTS OF SEED BUDS MINIMALLY AND FULLY
The present invention is a method of shoot apical meristem transformation for monocot plant via sufficient and micro wounding (SMW). The technical process includes: expose the apical meristem by removing the coleoptile away when the shoot grows to 0.2-2 cm after 1-2 days of seed germination; make su...
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| creator | SHI XUEPING CHAI JIANFANG ZHU JINYONG ZHAO HE YANG FAN REN ZHIHENG ZHANG QIANG GAO YIPING MA HUIJIE ZUO WENBO SUN GUOZHONG WANG HAIBO WANG ZHANWU LIANG XINCHAO XU XIAN GUAN JUNFENG ZHANG YANMIN LV MENGYU LIU YONGWEI DONG FUSHUANG HAN QIUFEN ZHANG HUANHUAN |
| description | The present invention is a method of shoot apical meristem transformation for monocot plant via sufficient and micro wounding (SMW). The technical process includes: expose the apical meristem by removing the coleoptile away when the shoot grows to 0.2-2 cm after 1-2 days of seed germination; make sufficient and micro wounding transformation to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 μm in diameter for each one and 0.5-3 mm in exposed length, and dipped with the Agrobacterium tumefaciens containing binary vector harboring exogenous genes; develop the treated meristems directly to normal plants after co-cultivation; promote the plants to develop big spikes and set more seeds; harvest the seeds of T0 plants separately; detect and identify the transformation results in T1 generation which is bred from each individual T0 plant. The advantages of the invention are independent of tissue culture, unlimited in genotype, unnecessary to carry resistant marker, simple and large scale to perform, and applicable to all monocot plants which can set seeds. The transformation efficiencies for wheat, rice and maize using this method are 49%, 66.3%, and 100%, respectively. |
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The technical process includes: expose the apical meristem by removing the coleoptile away when the shoot grows to 0.2-2 cm after 1-2 days of seed germination; make sufficient and micro wounding transformation to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 μm in diameter for each one and 0.5-3 mm in exposed length, and dipped with the Agrobacterium tumefaciens containing binary vector harboring exogenous genes; develop the treated meristems directly to normal plants after co-cultivation; promote the plants to develop big spikes and set more seeds; harvest the seeds of T0 plants separately; detect and identify the transformation results in T1 generation which is bred from each individual T0 plant. The advantages of the invention are independent of tissue culture, unlimited in genotype, unnecessary to carry resistant marker, simple and large scale to perform, and applicable to all monocot plants which can set seeds. The transformation efficiencies for wheat, rice and maize using this method are 49%, 66.3%, and 100%, respectively.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2015</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20151203&DB=EPODOC&CC=US&NR=2015344897A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20151203&DB=EPODOC&CC=US&NR=2015344897A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SHI XUEPING</creatorcontrib><creatorcontrib>CHAI JIANFANG</creatorcontrib><creatorcontrib>ZHU JINYONG</creatorcontrib><creatorcontrib>ZHAO HE</creatorcontrib><creatorcontrib>YANG FAN</creatorcontrib><creatorcontrib>REN ZHIHENG</creatorcontrib><creatorcontrib>ZHANG QIANG</creatorcontrib><creatorcontrib>GAO YIPING</creatorcontrib><creatorcontrib>MA HUIJIE</creatorcontrib><creatorcontrib>ZUO WENBO</creatorcontrib><creatorcontrib>SUN GUOZHONG</creatorcontrib><creatorcontrib>WANG HAIBO</creatorcontrib><creatorcontrib>WANG ZHANWU</creatorcontrib><creatorcontrib>LIANG XINCHAO</creatorcontrib><creatorcontrib>XU XIAN</creatorcontrib><creatorcontrib>GUAN JUNFENG</creatorcontrib><creatorcontrib>ZHANG YANMIN</creatorcontrib><creatorcontrib>LV MENGYU</creatorcontrib><creatorcontrib>LIU YONGWEI</creatorcontrib><creatorcontrib>DONG FUSHUANG</creatorcontrib><creatorcontrib>HAN QIUFEN</creatorcontrib><creatorcontrib>ZHANG HUANHUAN</creatorcontrib><title>MONOCOTYLEDON TRANSGENIC METHOD FOR INVADING GROWING POINTS OF SEED BUDS MINIMALLY AND FULLY</title><description>The present invention is a method of shoot apical meristem transformation for monocot plant via sufficient and micro wounding (SMW). The technical process includes: expose the apical meristem by removing the coleoptile away when the shoot grows to 0.2-2 cm after 1-2 days of seed germination; make sufficient and micro wounding transformation to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 μm in diameter for each one and 0.5-3 mm in exposed length, and dipped with the Agrobacterium tumefaciens containing binary vector harboring exogenous genes; develop the treated meristems directly to normal plants after co-cultivation; promote the plants to develop big spikes and set more seeds; harvest the seeds of T0 plants separately; detect and identify the transformation results in T1 generation which is bred from each individual T0 plant. The advantages of the invention are independent of tissue culture, unlimited in genotype, unnecessary to carry resistant marker, simple and large scale to perform, and applicable to all monocot plants which can set seeds. The transformation efficiencies for wheat, rice and maize using this method are 49%, 66.3%, and 100%, respectively.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2015</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNizsKwkAUANNYiHqHB9aCMRG1XPeXhex7sh8lIIQgayUaiPfHCB7AaqaYmWZXS0icQlNLQQjBMfRaouFgZahIgCIHBs9MGNSgHV2-PJHB4IEUeCkFHKPwYA0ay-q6AYbjFkebZ5N79xjS4sdZtlQy8GqV-lebhr67pWd6t9Fv1vm2KMv9Ycfy4r_qA6j8M4Y</recordid><startdate>20151203</startdate><enddate>20151203</enddate><creator>SHI XUEPING</creator><creator>CHAI JIANFANG</creator><creator>ZHU JINYONG</creator><creator>ZHAO HE</creator><creator>YANG FAN</creator><creator>REN ZHIHENG</creator><creator>ZHANG QIANG</creator><creator>GAO YIPING</creator><creator>MA HUIJIE</creator><creator>ZUO WENBO</creator><creator>SUN GUOZHONG</creator><creator>WANG HAIBO</creator><creator>WANG ZHANWU</creator><creator>LIANG XINCHAO</creator><creator>XU XIAN</creator><creator>GUAN JUNFENG</creator><creator>ZHANG YANMIN</creator><creator>LV MENGYU</creator><creator>LIU YONGWEI</creator><creator>DONG FUSHUANG</creator><creator>HAN QIUFEN</creator><creator>ZHANG HUANHUAN</creator><scope>EVB</scope></search><sort><creationdate>20151203</creationdate><title>MONOCOTYLEDON TRANSGENIC METHOD FOR INVADING GROWING POINTS OF SEED BUDS MINIMALLY AND FULLY</title><author>SHI XUEPING ; CHAI JIANFANG ; ZHU JINYONG ; ZHAO HE ; YANG FAN ; REN ZHIHENG ; ZHANG QIANG ; GAO YIPING ; MA HUIJIE ; ZUO WENBO ; SUN GUOZHONG ; WANG HAIBO ; WANG ZHANWU ; LIANG XINCHAO ; XU XIAN ; GUAN JUNFENG ; ZHANG YANMIN ; LV MENGYU ; LIU YONGWEI ; DONG FUSHUANG ; HAN QIUFEN ; ZHANG HUANHUAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US2015344897A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2015</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>SHI XUEPING</creatorcontrib><creatorcontrib>CHAI JIANFANG</creatorcontrib><creatorcontrib>ZHU JINYONG</creatorcontrib><creatorcontrib>ZHAO HE</creatorcontrib><creatorcontrib>YANG FAN</creatorcontrib><creatorcontrib>REN ZHIHENG</creatorcontrib><creatorcontrib>ZHANG QIANG</creatorcontrib><creatorcontrib>GAO YIPING</creatorcontrib><creatorcontrib>MA HUIJIE</creatorcontrib><creatorcontrib>ZUO WENBO</creatorcontrib><creatorcontrib>SUN GUOZHONG</creatorcontrib><creatorcontrib>WANG HAIBO</creatorcontrib><creatorcontrib>WANG ZHANWU</creatorcontrib><creatorcontrib>LIANG XINCHAO</creatorcontrib><creatorcontrib>XU XIAN</creatorcontrib><creatorcontrib>GUAN JUNFENG</creatorcontrib><creatorcontrib>ZHANG YANMIN</creatorcontrib><creatorcontrib>LV MENGYU</creatorcontrib><creatorcontrib>LIU YONGWEI</creatorcontrib><creatorcontrib>DONG FUSHUANG</creatorcontrib><creatorcontrib>HAN QIUFEN</creatorcontrib><creatorcontrib>ZHANG HUANHUAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SHI XUEPING</au><au>CHAI JIANFANG</au><au>ZHU JINYONG</au><au>ZHAO HE</au><au>YANG FAN</au><au>REN ZHIHENG</au><au>ZHANG QIANG</au><au>GAO YIPING</au><au>MA HUIJIE</au><au>ZUO WENBO</au><au>SUN GUOZHONG</au><au>WANG HAIBO</au><au>WANG ZHANWU</au><au>LIANG XINCHAO</au><au>XU XIAN</au><au>GUAN JUNFENG</au><au>ZHANG YANMIN</au><au>LV MENGYU</au><au>LIU YONGWEI</au><au>DONG FUSHUANG</au><au>HAN QIUFEN</au><au>ZHANG HUANHUAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>MONOCOTYLEDON TRANSGENIC METHOD FOR INVADING GROWING POINTS OF SEED BUDS MINIMALLY AND FULLY</title><date>2015-12-03</date><risdate>2015</risdate><abstract>The present invention is a method of shoot apical meristem transformation for monocot plant via sufficient and micro wounding (SMW). The technical process includes: expose the apical meristem by removing the coleoptile away when the shoot grows to 0.2-2 cm after 1-2 days of seed germination; make sufficient and micro wounding transformation to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 μm in diameter for each one and 0.5-3 mm in exposed length, and dipped with the Agrobacterium tumefaciens containing binary vector harboring exogenous genes; develop the treated meristems directly to normal plants after co-cultivation; promote the plants to develop big spikes and set more seeds; harvest the seeds of T0 plants separately; detect and identify the transformation results in T1 generation which is bred from each individual T0 plant. The advantages of the invention are independent of tissue culture, unlimited in genotype, unnecessary to carry resistant marker, simple and large scale to perform, and applicable to all monocot plants which can set seeds. The transformation efficiencies for wheat, rice and maize using this method are 49%, 66.3%, and 100%, respectively.</abstract><oa>free_for_read</oa></addata></record> |
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| title | MONOCOTYLEDON TRANSGENIC METHOD FOR INVADING GROWING POINTS OF SEED BUDS MINIMALLY AND FULLY |
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