SPLIT INTEINS AND USES THEREOF

The present invention relates generally to robust split inteins. The split inteins described herein are active over a large temperature range, including temperatures as low as 0° C., over a wide pH range, and in the presence of chaotropic salts. The split inteins also show high tolerance to sequence...

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Hauptverfasser: MARCO FELIU DÍDAC, SCHMIDT STEFAN ROBERT, CARVAJAL VALLEJOS PATRICIA KARINA, PALLISSE BERGWERF ROSER
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creator MARCO FELIU DÍDAC
SCHMIDT STEFAN ROBERT
CARVAJAL VALLEJOS PATRICIA KARINA
PALLISSE BERGWERF ROSER
description The present invention relates generally to robust split inteins. The split inteins described herein are active over a large temperature range, including temperatures as low as 0° C., over a wide pH range, and in the presence of chaotropic salts. The split inteins also show high tolerance to sequence variability in fused heterologous polypeptides and therefore are useful in protein purification and engineering techniques.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
SPIRITS
VINEGAR
WINE
title SPLIT INTEINS AND USES THEREOF
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