Process for the Purification of Recombinant Granulocyte-Colony Stimulating Factor

Process for the Purification of Recombinant Granulocyte-Colony Stimulating Factor

The present invention describes a novel process for large scale purification of therapeutic grade quality of recombinant human G-CSF from microbial cells, wherein the protein is expressed as inclusion bodies. The process involves the novel use of Hydrophobic Interaction Chromatography (HIC) step to...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: SAMADDAR MITALI, NANDAMURI ANUPAMA, NUVVULA ASHOK KUMAR, CHIGURUPATI JAYARAM, KOMATH UMA DEVI, KARRA SREENIVASU, MOVVA SRILALITHA
Format: Patent
Sprache:eng
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator SAMADDAR MITALI
NANDAMURI ANUPAMA
NUVVULA ASHOK KUMAR
CHIGURUPATI JAYARAM
KOMATH UMA DEVI
KARRA SREENIVASU
MOVVA SRILALITHA
description The present invention describes a novel process for large scale purification of therapeutic grade quality of recombinant human G-CSF from microbial cells, wherein the protein is expressed as inclusion bodies. The process involves the novel use of Hydrophobic Interaction Chromatography (HIC) step to purify G-CSF eluted from a cation exchange column. A combination of these two chromatography steps provides good purity and yields which are essential for a production scale process. The host cell related contaminants like proteins, DNA and endotoxins are estimated to be within the specifications outlined by the drug regulatory authorities.
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_US2008171857A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US2008171857A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_US2008171857A13</originalsourceid><addsrcrecordid>eNqNyrEKwjAQANAsDqL-w4FzoVGkXaVYHavVucRw0UB6V5LL0L_XwQ9westbqmsX2WJK4DiCvBG6HL3z1ohnAnZwQ8vj05MhgXM0lAPbWbBoODDN0Isfc_huekFrrHBcq4UzIeHm50pt29O9uRQ48YBpMhYJZXj0u7KsdaXrQ3XU-__WB9PHOPM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Process for the Purification of Recombinant Granulocyte-Colony Stimulating Factor</title><source>esp@cenet</source><creator>SAMADDAR MITALI ; NANDAMURI ANUPAMA ; NUVVULA ASHOK KUMAR ; CHIGURUPATI JAYARAM ; KOMATH UMA DEVI ; KARRA SREENIVASU ; MOVVA SRILALITHA</creator><creatorcontrib>SAMADDAR MITALI ; NANDAMURI ANUPAMA ; NUVVULA ASHOK KUMAR ; CHIGURUPATI JAYARAM ; KOMATH UMA DEVI ; KARRA SREENIVASU ; MOVVA SRILALITHA</creatorcontrib><description>The present invention describes a novel process for large scale purification of therapeutic grade quality of recombinant human G-CSF from microbial cells, wherein the protein is expressed as inclusion bodies. The process involves the novel use of Hydrophobic Interaction Chromatography (HIC) step to purify G-CSF eluted from a cation exchange column. A combination of these two chromatography steps provides good purity and yields which are essential for a production scale process. The host cell related contaminants like proteins, DNA and endotoxins are estimated to be within the specifications outlined by the drug regulatory authorities.</description><language>eng</language><subject>CHEMISTRY ; METALLURGY ; ORGANIC CHEMISTRY ; PEPTIDES</subject><creationdate>2008</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20080717&amp;DB=EPODOC&amp;CC=US&amp;NR=2008171857A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20080717&amp;DB=EPODOC&amp;CC=US&amp;NR=2008171857A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SAMADDAR MITALI</creatorcontrib><creatorcontrib>NANDAMURI ANUPAMA</creatorcontrib><creatorcontrib>NUVVULA ASHOK KUMAR</creatorcontrib><creatorcontrib>CHIGURUPATI JAYARAM</creatorcontrib><creatorcontrib>KOMATH UMA DEVI</creatorcontrib><creatorcontrib>KARRA SREENIVASU</creatorcontrib><creatorcontrib>MOVVA SRILALITHA</creatorcontrib><title>Process for the Purification of Recombinant Granulocyte-Colony Stimulating Factor</title><description>The present invention describes a novel process for large scale purification of therapeutic grade quality of recombinant human G-CSF from microbial cells, wherein the protein is expressed as inclusion bodies. The process involves the novel use of Hydrophobic Interaction Chromatography (HIC) step to purify G-CSF eluted from a cation exchange column. A combination of these two chromatography steps provides good purity and yields which are essential for a production scale process. The host cell related contaminants like proteins, DNA and endotoxins are estimated to be within the specifications outlined by the drug regulatory authorities.</description><subject>CHEMISTRY</subject><subject>METALLURGY</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2008</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyrEKwjAQANAsDqL-w4FzoVGkXaVYHavVucRw0UB6V5LL0L_XwQ9westbqmsX2WJK4DiCvBG6HL3z1ohnAnZwQ8vj05MhgXM0lAPbWbBoODDN0Isfc_huekFrrHBcq4UzIeHm50pt29O9uRQ48YBpMhYJZXj0u7KsdaXrQ3XU-__WB9PHOPM</recordid><startdate>20080717</startdate><enddate>20080717</enddate><creator>SAMADDAR MITALI</creator><creator>NANDAMURI ANUPAMA</creator><creator>NUVVULA ASHOK KUMAR</creator><creator>CHIGURUPATI JAYARAM</creator><creator>KOMATH UMA DEVI</creator><creator>KARRA SREENIVASU</creator><creator>MOVVA SRILALITHA</creator><scope>EVB</scope></search><sort><creationdate>20080717</creationdate><title>Process for the Purification of Recombinant Granulocyte-Colony Stimulating Factor</title><author>SAMADDAR MITALI ; NANDAMURI ANUPAMA ; NUVVULA ASHOK KUMAR ; CHIGURUPATI JAYARAM ; KOMATH UMA DEVI ; KARRA SREENIVASU ; MOVVA SRILALITHA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US2008171857A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2008</creationdate><topic>CHEMISTRY</topic><topic>METALLURGY</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><toplevel>online_resources</toplevel><creatorcontrib>SAMADDAR MITALI</creatorcontrib><creatorcontrib>NANDAMURI ANUPAMA</creatorcontrib><creatorcontrib>NUVVULA ASHOK KUMAR</creatorcontrib><creatorcontrib>CHIGURUPATI JAYARAM</creatorcontrib><creatorcontrib>KOMATH UMA DEVI</creatorcontrib><creatorcontrib>KARRA SREENIVASU</creatorcontrib><creatorcontrib>MOVVA SRILALITHA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SAMADDAR MITALI</au><au>NANDAMURI ANUPAMA</au><au>NUVVULA ASHOK KUMAR</au><au>CHIGURUPATI JAYARAM</au><au>KOMATH UMA DEVI</au><au>KARRA SREENIVASU</au><au>MOVVA SRILALITHA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Process for the Purification of Recombinant Granulocyte-Colony Stimulating Factor</title><date>2008-07-17</date><risdate>2008</risdate><abstract>The present invention describes a novel process for large scale purification of therapeutic grade quality of recombinant human G-CSF from microbial cells, wherein the protein is expressed as inclusion bodies. The process involves the novel use of Hydrophobic Interaction Chromatography (HIC) step to purify G-CSF eluted from a cation exchange column. A combination of these two chromatography steps provides good purity and yields which are essential for a production scale process. The host cell related contaminants like proteins, DNA and endotoxins are estimated to be within the specifications outlined by the drug regulatory authorities.</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng
recordid cdi_epo_espacenet_US2008171857A1
source esp@cenet
subjects CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
title Process for the Purification of Recombinant Granulocyte-Colony Stimulating Factor
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T22%3A01%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=SAMADDAR%20MITALI&rft.date=2008-07-17&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EUS2008171857A1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true