Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry
The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken from multiple cell culture batches or of samples taken at different times from a given batch. The invention additiona...
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| creator | LOMAS LEE O BOSCHETTI EGISTO YIP TAI-TUNG BRADBURY LISA PHAM THANG T THULASIRAMAN VANITHA DAVIES HUW |
| description | The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken from multiple cell culture batches or of samples taken at different times from a given batch. The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process. |
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The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2007</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20070315&DB=EPODOC&CC=US&NR=2007059776A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20070315&DB=EPODOC&CC=US&NR=2007059776A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LOMAS LEE O</creatorcontrib><creatorcontrib>BOSCHETTI EGISTO</creatorcontrib><creatorcontrib>YIP TAI-TUNG</creatorcontrib><creatorcontrib>BRADBURY LISA</creatorcontrib><creatorcontrib>PHAM THANG T</creatorcontrib><creatorcontrib>THULASIRAMAN VANITHA</creatorcontrib><creatorcontrib>DAVIES HUW</creatorcontrib><title>Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry</title><description>The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken from multiple cell culture batches or of samples taken at different times from a given batch. The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2007</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjLEKwkAQRNNYiPoPC9ZiVDRYiig2Vmotx92eOUhul91LEVt_3ET9AIvhwfBmhtnrjKkkp-BJoKYYEkmID2CqWkZOwSGwkGtsChTBRAfcSPDBmk_RaG9rI95YBIyliRYdVEZRwKGScO_Nu4Tnd1IbVVBGm4RqTNKOs4E3leLkx1E2PR6u-9MMme6o3D1HTPfbZZnnRb7eFsVmt1j9Z70BqpZNpg</recordid><startdate>20070315</startdate><enddate>20070315</enddate><creator>LOMAS LEE O</creator><creator>BOSCHETTI EGISTO</creator><creator>YIP TAI-TUNG</creator><creator>BRADBURY LISA</creator><creator>PHAM THANG T</creator><creator>THULASIRAMAN VANITHA</creator><creator>DAVIES HUW</creator><scope>EVB</scope></search><sort><creationdate>20070315</creationdate><title>Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry</title><author>LOMAS LEE O ; BOSCHETTI EGISTO ; YIP TAI-TUNG ; BRADBURY LISA ; PHAM THANG T ; THULASIRAMAN VANITHA ; DAVIES HUW</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US2007059776A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2007</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LOMAS LEE O</creatorcontrib><creatorcontrib>BOSCHETTI EGISTO</creatorcontrib><creatorcontrib>YIP TAI-TUNG</creatorcontrib><creatorcontrib>BRADBURY LISA</creatorcontrib><creatorcontrib>PHAM THANG T</creatorcontrib><creatorcontrib>THULASIRAMAN VANITHA</creatorcontrib><creatorcontrib>DAVIES HUW</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LOMAS LEE O</au><au>BOSCHETTI EGISTO</au><au>YIP TAI-TUNG</au><au>BRADBURY LISA</au><au>PHAM THANG T</au><au>THULASIRAMAN VANITHA</au><au>DAVIES HUW</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry</title><date>2007-03-15</date><risdate>2007</risdate><abstract>The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken from multiple cell culture batches or of samples taken at different times from a given batch. The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS SPIRITS TESTING VINEGAR WINE |
| title | Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry |
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