KINASE AND PHOSPHATASE ACTIVITY MEASUREMENT USING FLUORESCENT POLARIZATION

A method for quantitating enzyme activity that causes phosphorylation or dephosphorylation of a peptide or protein substrate is provided. The method utilizes the differences in fluorescence polarization of a phosphorylated amino acid-containing fluorescence-emitting reporter molecule free in solutio...

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Hauptverfasser: BOLGER RANDALL E, BURKE THOMAS J, PARKER GREGORY, SCHALL REBECCA
Format: Patent
Sprache:eng
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Zusammenfassung:A method for quantitating enzyme activity that causes phosphorylation or dephosphorylation of a peptide or protein substrate is provided. The method utilizes the differences in fluorescence polarization of a phosphorylated amino acid-containing fluorescence-emitting reporter molecule free in solution as compared to being bound to a binding molecule.