DNAS ENCODING NEW FUSION PROTEINS AND PROCESSES FOR PREPARING USEFUL POLYPEPTIDES THROUGH EXPRESSION OF THE DNAS
This invention relates to a DNA comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises: a sequence of signal peptide for Bacillus cell wall protein (CWP); a tag sequence for separation and purification of the fusion protein; a linker sequence; a sequence for...
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| creator | KONDO MASAAKI SATO SEIJI HIGASHIKUNI NAOHIKO KUDO TOSHIYUKI |
| description | This invention relates to a DNA comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises: a sequence of signal peptide for Bacillus cell wall protein (CWP); a tag sequence for separation and purification of the fusion protein; a linker sequence; a sequence for chemical or enzymatic cleavage; and an exogenous polypeptide sequence, said sequences being linked in order, said signal peptide, tag and linker being optional sequences; and wherein said nucleotide sequence encoding a fusion protein is ligated to 3'-end of a nucleic acid sequence comprising a Bacillus promoter region; to a vector comprising the DNA; to a bacterium belonging to the genus Bacillus comprising the vector; and to a process for preparation of a useful polypeptide by culture of the bacterium. |
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a tag sequence for separation and purification of the fusion protein; a linker sequence; a sequence for chemical or enzymatic cleavage; and an exogenous polypeptide sequence, said sequences being linked in order, said signal peptide, tag and linker being optional sequences; and wherein said nucleotide sequence encoding a fusion protein is ligated to 3'-end of a nucleic acid sequence comprising a Bacillus promoter region; to a vector comprising the DNA; to a bacterium belonging to the genus Bacillus comprising the vector; and to a process for preparation of a useful polypeptide by culture of the bacterium.</description><edition>7</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2001</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20010913&DB=EPODOC&CC=US&NR=2001021515A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25547,76298</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20010913&DB=EPODOC&CC=US&NR=2001021515A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KONDO MASAAKI</creatorcontrib><creatorcontrib>SATO SEIJI</creatorcontrib><creatorcontrib>HIGASHIKUNI NAOHIKO</creatorcontrib><creatorcontrib>KUDO TOSHIYUKI</creatorcontrib><title>DNAS ENCODING NEW FUSION PROTEINS AND PROCESSES FOR PREPARING USEFUL POLYPEPTIDES THROUGH EXPRESSION OF THE DNAS</title><description>This invention relates to a DNA comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises: a sequence of signal peptide for Bacillus cell wall protein (CWP); a tag sequence for separation and purification of the fusion protein; a linker sequence; a sequence for chemical or enzymatic cleavage; and an exogenous polypeptide sequence, said sequences being linked in order, said signal peptide, tag and linker being optional sequences; and wherein said nucleotide sequence encoding a fusion protein is ligated to 3'-end of a nucleic acid sequence comprising a Bacillus promoter region; to a vector comprising the DNA; to a bacterium belonging to the genus Bacillus comprising the vector; and to a process for preparation of a useful polypeptide by culture of the bacterium.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2001</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjUEKwjAQRbtxIeodBlwLTaUHCM2kDZRMyCRYV6VIXIkW6v0xFQ_g6vM-j_-3xaysZEDbkDK2BYsX0JENWXCeAhrLIK1aoUFmZNDkM6GTfvUjo449OOqvDl0wKhuh8xTbDnDIHn-3SOcWYf3aF5v79FjS4Ze74qgxNN0pza8xLfN0S8_0HiNXZSnKStSiluL8n_UB6C85CA</recordid><startdate>20010913</startdate><enddate>20010913</enddate><creator>KONDO MASAAKI</creator><creator>SATO SEIJI</creator><creator>HIGASHIKUNI NAOHIKO</creator><creator>KUDO TOSHIYUKI</creator><scope>EVB</scope></search><sort><creationdate>20010913</creationdate><title>DNAS ENCODING NEW FUSION PROTEINS AND PROCESSES FOR PREPARING USEFUL POLYPEPTIDES THROUGH EXPRESSION OF THE DNAS</title><author>KONDO MASAAKI ; SATO SEIJI ; HIGASHIKUNI NAOHIKO ; KUDO TOSHIYUKI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US2001021515A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2001</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KONDO MASAAKI</creatorcontrib><creatorcontrib>SATO SEIJI</creatorcontrib><creatorcontrib>HIGASHIKUNI NAOHIKO</creatorcontrib><creatorcontrib>KUDO TOSHIYUKI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KONDO MASAAKI</au><au>SATO SEIJI</au><au>HIGASHIKUNI NAOHIKO</au><au>KUDO TOSHIYUKI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>DNAS ENCODING NEW FUSION PROTEINS AND PROCESSES FOR PREPARING USEFUL POLYPEPTIDES THROUGH EXPRESSION OF THE DNAS</title><date>2001-09-13</date><risdate>2001</risdate><abstract>This invention relates to a DNA comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises: a sequence of signal peptide for Bacillus cell wall protein (CWP); a tag sequence for separation and purification of the fusion protein; a linker sequence; a sequence for chemical or enzymatic cleavage; and an exogenous polypeptide sequence, said sequences being linked in order, said signal peptide, tag and linker being optional sequences; and wherein said nucleotide sequence encoding a fusion protein is ligated to 3'-end of a nucleic acid sequence comprising a Bacillus promoter region; to a vector comprising the DNA; to a bacterium belonging to the genus Bacillus comprising the vector; and to a process for preparation of a useful polypeptide by culture of the bacterium.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | DNAS ENCODING NEW FUSION PROTEINS AND PROCESSES FOR PREPARING USEFUL POLYPEPTIDES THROUGH EXPRESSION OF THE DNAS |
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