Systems and methods for fractionation and collection of analytes in a sample

Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end i...

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Hauptverfasser: Yang, Tom Weisan, Zhou, Cheng, Dermody, Jessica, Roach, David J, Wang, Roland Y, Xu, Hui, Wu, Jiaqi
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creator Yang, Tom Weisan
Zhou, Cheng
Dermody, Jessica
Roach, David J
Wang, Roland Y
Xu, Hui
Wu, Jiaqi
description Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.
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subjects CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
TRANSPORTING
title Systems and methods for fractionation and collection of analytes in a sample
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