Preparation of a sample for high pressure freezing

A method including one or more steps of a procedure for preparing a microscopic sample for a high-pressure freezing process is provided, wherein the sample is provided using an arrangement comprising a middle plate of a high-pressure freezing cartridge and an incubation chamber, wherein the middle p...

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Hauptverfasser: Kreutzer, Joose, Wogritsch, Rainer, Wurzinger, Paul
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creator Kreutzer, Joose
Wogritsch, Rainer
Wurzinger, Paul
description A method including one or more steps of a procedure for preparing a microscopic sample for a high-pressure freezing process is provided, wherein the sample is provided using an arrangement comprising a middle plate of a high-pressure freezing cartridge and an incubation chamber, wherein the middle plate is attached to a lower surface of a bottom of the incubation chamber by an adhesive and detachable from the incubation chamber by effecting a relative movement between the middle plate and the incubation chamber, wherein the sample is provided on an enclosing element which is fitted into an opening of the middle plate, and wherein, when the sample is to be subjected to the high-pressure freezing process, the relative movement between the middle plate and the incubation chamber is effected, thereby detaching the middle plate with the sample from the incubation chamber. Means to implement the method are also part of the present disclosure.
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subjects APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
BLASTING
CHEMISTRY
COLD ROOMS
COMBINED HEATING AND REFRIGERATION SYSTEMS
COOLING OR FREEZING APPARATUS NOT COVERED BY ANY OTHERSUBCLASS
ENZYMOLOGY
HEAT PUMP SYSTEMS
HEATING
ICE-BOXES
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
LIGHTING
LIQUEFACTION SOLIDIFICATION OF GASES
MANUFACTURE OR STORAGE OF ICE
MEASURING
MECHANICAL ENGINEERING
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
REFRIGERATION OR COOLING
REFRIGERATORS
SPIRITS
TESTING
VINEGAR
WEAPONS
WINE
title Preparation of a sample for high pressure freezing
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