Saccharomyces cerevisiae strain with high yield of ethyl butyrate and construction method and application of Saccharomyces cerevisiae strain
A Saccharomyces cerevisiae strain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3...
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| creator | Du, Yongjing Chen, Yefu Guo, Xuewu Ren, Jinying Wu, Xiaole Jiang, Sen Ma, Yanrui Zhang, Guo Zheng, Peng Xiao, Dongguang Kang, Xinyue |
| description | A Saccharomyces cerevisiae strain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33±3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65±7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93±3.18 mg/L of ethyl crotonate is unexpectedly produced. |
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The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33±3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65±7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93±3.18 mg/L of ethyl crotonate is unexpectedly produced.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; OTHER ALCOHOLIC BEVERAGES ; PREPARATION THEREOF ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230905&DB=EPODOC&CC=US&NR=11746353B2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230905&DB=EPODOC&CC=US&NR=11746353B2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Du, Yongjing</creatorcontrib><creatorcontrib>Chen, Yefu</creatorcontrib><creatorcontrib>Guo, Xuewu</creatorcontrib><creatorcontrib>Ren, Jinying</creatorcontrib><creatorcontrib>Wu, Xiaole</creatorcontrib><creatorcontrib>Jiang, Sen</creatorcontrib><creatorcontrib>Ma, Yanrui</creatorcontrib><creatorcontrib>Zhang, Guo</creatorcontrib><creatorcontrib>Zheng, Peng</creatorcontrib><creatorcontrib>Xiao, Dongguang</creatorcontrib><creatorcontrib>Kang, Xinyue</creatorcontrib><title>Saccharomyces cerevisiae strain with high yield of ethyl butyrate and construction method and application of Saccharomyces cerevisiae strain</title><description>A Saccharomyces cerevisiae strain with high yield of ethyl butyrate and a construction method and an application thereof are provided. 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Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33±3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65±7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93±3.18 mg/L of ethyl crotonate is unexpectedly produced.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>OTHER ALCOHOLIC BEVERAGES</subject><subject>PREPARATION THEREOF</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjTsOwjAQBdNQIOAOywEoQvj0IBB9oI6WzQav5NiW7YB8Bw6NFdFD9aTRjN60eNdIpNDbPhEHIPb8lCDIEKJHMfCSqEDJQ0ES1i3YDjiqpOE-xOQxMqBpgazJ_kBRrIE-C7YdOTqnhXDEufxxNi8mHerAi-_OiuX5dD1eVuxsw8EhseHY3Oqy3G921bY6rKt_nA_YiU8I</recordid><startdate>20230905</startdate><enddate>20230905</enddate><creator>Du, Yongjing</creator><creator>Chen, Yefu</creator><creator>Guo, Xuewu</creator><creator>Ren, Jinying</creator><creator>Wu, Xiaole</creator><creator>Jiang, Sen</creator><creator>Ma, Yanrui</creator><creator>Zhang, Guo</creator><creator>Zheng, Peng</creator><creator>Xiao, Dongguang</creator><creator>Kang, Xinyue</creator><scope>EVB</scope></search><sort><creationdate>20230905</creationdate><title>Saccharomyces cerevisiae strain with high yield of ethyl butyrate and construction method and application of Saccharomyces cerevisiae strain</title><author>Du, Yongjing ; Chen, Yefu ; Guo, Xuewu ; Ren, Jinying ; Wu, Xiaole ; Jiang, Sen ; Ma, Yanrui ; Zhang, Guo ; Zheng, Peng ; Xiao, Dongguang ; Kang, Xinyue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US11746353B23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>OTHER ALCOHOLIC BEVERAGES</topic><topic>PREPARATION THEREOF</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Du, Yongjing</creatorcontrib><creatorcontrib>Chen, Yefu</creatorcontrib><creatorcontrib>Guo, Xuewu</creatorcontrib><creatorcontrib>Ren, Jinying</creatorcontrib><creatorcontrib>Wu, Xiaole</creatorcontrib><creatorcontrib>Jiang, Sen</creatorcontrib><creatorcontrib>Ma, Yanrui</creatorcontrib><creatorcontrib>Zhang, Guo</creatorcontrib><creatorcontrib>Zheng, Peng</creatorcontrib><creatorcontrib>Xiao, Dongguang</creatorcontrib><creatorcontrib>Kang, Xinyue</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Du, Yongjing</au><au>Chen, Yefu</au><au>Guo, Xuewu</au><au>Ren, Jinying</au><au>Wu, Xiaole</au><au>Jiang, Sen</au><au>Ma, Yanrui</au><au>Zhang, Guo</au><au>Zheng, Peng</au><au>Xiao, Dongguang</au><au>Kang, Xinyue</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Saccharomyces cerevisiae strain with high yield of ethyl butyrate and construction method and application of Saccharomyces cerevisiae strain</title><date>2023-09-05</date><risdate>2023</risdate><abstract>A Saccharomyces cerevisiae strain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33±3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65±7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93±3.18 mg/L of ethyl crotonate is unexpectedly produced.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING OTHER ALCOHOLIC BEVERAGES PREPARATION THEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Saccharomyces cerevisiae strain with high yield of ethyl butyrate and construction method and application of Saccharomyces cerevisiae strain |
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