Apparatus and method for fast volumetric fluorescence microscopy using temporally multiplexed light sheets

Apparatus and method for fast volumetric fluorescence microscopy using temporally multiplexed light sheets

A microscopy device comprises a continuous or pulsed wave laser light source; a pair of parallel mirrors configured to receive light from the light source and reflect an array of incoherent light sheets; a beam encoder (e.g., frequency modulation reticle, Hadamard basis, random modulation pattern) t...

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Hauptverfasser: Ren, Yuxuan, Wu, Jianglai, Lai, Queenie Tsz Kwan, Tsia, Kevin Kin Man, Lau, Andy Kam Seng
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
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OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
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creator Ren, Yuxuan
Wu, Jianglai
Lai, Queenie Tsz Kwan
Tsia, Kevin Kin Man
Lau, Andy Kam Seng
description A microscopy device comprises a continuous or pulsed wave laser light source; a pair of parallel mirrors configured to receive light from the light source and reflect an array of incoherent light sheets; a beam encoder (e.g., frequency modulation reticle, Hadamard basis, random modulation pattern) to segment the array of incoherent light sheets and encode each light sheet with a respective frequency in reciprocal space; a lens configured to direct the encoded light sheets towards a biological sample; and an image capturing device configured to receive a fluorescence signal from the biological sample.
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
OPTICS
PHYSICS
TESTING
title Apparatus and method for fast volumetric fluorescence microscopy using temporally multiplexed light sheets
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