System including a member configured to separate a lock insert from a guide hole in a top nozzle plate of a nuclear fuel assembly

System for separating and coupling a nuclear fuel assembly from/to a top nozzle which has a flow channel plate with guide holes. The system includes a lock insert and a separation member. The lock insert includes an insertion part provided on a top portion of a hollow body. The separation member is configured to separate the insertion part from a guide hole. The insertion part is variable in size. The insertion part comprises a first latching member and a second latching member, each having a step which contacts the flow channel plate. The first latching member includes a latching groove which is inserted into a member protruding from the top surface of the flow channel plate. The second latching member contacts a bottom surface of the flow channel plate. The separation member provides a space accommodating an outer circumferential surface of the first latching member.