Method for producing cellulase and apparatus for said method

A method of producing cellulase includes steps (1) to (3): (1) subjecting an aqueous solution of cellulase derived from filamentous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and concurrently obtain a concentrate...

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Bibliographische Detailangaben
Hauptverfasser:	Yamada, Katsushige, Kurihara, Hiroyuki
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SEPARATION SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of producing cellulase includes steps (1) to (3): (1) subjecting an aqueous solution of cellulase derived from filamentous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and concurrently obtain a concentrated enzyme liquid as a retentate; (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 to 50,000 to obtain a second concentrated enzyme liquid as a retentate; and (3) mixing the concentrated enzyme liquid obtained in steps (1) and (2) to obtain cellulase derived from filamentous fungi.