Photocoupling method using probe containing photoresponsive nucleic acids
Provided are: a photocoupling method that overcomes the problem of the stagnation of photocoupling with a target nucleotide using a probe containing a photo-responsive nucleotide, and that improves the photocoupling efficiency; and a photocoupling kit.The photocoupling method is characterized by hyb...
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| creator | Wakamatsu, Hirotake Fujimoto, Kenzo Fugono, Nobutake Shimadzu, Mitsunobu Yanagihara, Akira Terasaki, Hiroshi |
| description | Provided are: a photocoupling method that overcomes the problem of the stagnation of photocoupling with a target nucleotide using a probe containing a photo-responsive nucleotide, and that improves the photocoupling efficiency; and a photocoupling kit.The photocoupling method is characterized by hybridizing a target site present in a nucleic acid sample with a first probe having a sequence complementary to the target site and containing a photo-responsive nucleotide, in a reaction solution, and carrying out photocoupling by photo-irradiation, wherein self-assembly caused by the photo-responsive nucleotide contained in the first probe is suppressed. The photocoupling kit is characterized by comprising a first probe having a sequence complementary to a target site present in a nucleic acid sample, and containing a photo-responsive nucleotide; and a second probe being highly complementary to the first probe. |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Photocoupling method using probe containing photoresponsive nucleic acids |
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