Methods for sorting nucleic acids and multiplexed preparative in vitro cloning

Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids using high throughput sequencing. Specifically, nucleic acid molecules having the desired predetermined sequence can be sorted from a pool comprising a plurality of nucleic acids having correct and incorrect se...

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Hauptverfasser: Goldberg, Martin J, Hudson, Michael E, Jacobson, Joseph, Kung, Li-Yun A, Schindler, Daniel
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Sprache:eng
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creator Goldberg, Martin J
Hudson, Michael E
Jacobson, Joseph
Kung, Li-Yun A
Schindler, Daniel
description Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids using high throughput sequencing. Specifically, nucleic acid molecules having the desired predetermined sequence can be sorted from a pool comprising a plurality of nucleic acids having correct and incorrect sequences.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
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