Generation of novel metabolite-responsive transcription regulator biosensors
Disclosed are systems, components, and methods for sensing a ligand in a cell or a reaction mixture. The disclosed systems, components, and methods may include and/or utilize a fusion protein comprising a ligand-binding protein and a DNA-binding protein. The fusion protein binds the ligand of the li...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Patent |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | Younger, Andrew K. D Tyo, Keith E. J Leonard, Joshua N |
| description | Disclosed are systems, components, and methods for sensing a ligand in a cell or a reaction mixture. The disclosed systems, components, and methods may include and/or utilize a fusion protein comprising a ligand-binding protein and a DNA-binding protein. The fusion protein binds the ligand of the ligand-binding protein and modulates expression of a reporter gene operably linked to a promoter that is engineered to include specific binding sites for the DNA-binding protein. The difference in expression of the reporter gene in the presence of the ligand versus expression of the reporter gene in the absence of the ligand can be correlated to the concentration of the ligand in a reaction mixture. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_US10604552B2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US10604552B2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_US10604552B23</originalsourceid><addsrcrecordid>eNqNjUsKwkAMQGfjQtQ7jAco1Go9gOJn4U5dl7SkJTAmQxJ7fot4AFdv83hvHm4XZFRwEo7SR5YRU3yhQyuJHAtFy8JGI0ZXYOuU8ldWHN4JXDS2JIZsorYMsx6S4erHRVifT4_jtcAszRSCbpp587xvyn25q-vqUG3_cT5IjTdn</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Generation of novel metabolite-responsive transcription regulator biosensors</title><source>esp@cenet</source><creator>Younger, Andrew K. D ; Tyo, Keith E. J ; Leonard, Joshua N</creator><creatorcontrib>Younger, Andrew K. D ; Tyo, Keith E. J ; Leonard, Joshua N</creatorcontrib><description>Disclosed are systems, components, and methods for sensing a ligand in a cell or a reaction mixture. The disclosed systems, components, and methods may include and/or utilize a fusion protein comprising a ligand-binding protein and a DNA-binding protein. The fusion protein binds the ligand of the ligand-binding protein and modulates expression of a reporter gene operably linked to a promoter that is engineered to include specific binding sites for the DNA-binding protein. The difference in expression of the reporter gene in the presence of the ligand versus expression of the reporter gene in the absence of the ligand can be correlated to the concentration of the ligand in a reaction mixture.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200331&DB=EPODOC&CC=US&NR=10604552B2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200331&DB=EPODOC&CC=US&NR=10604552B2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Younger, Andrew K. D</creatorcontrib><creatorcontrib>Tyo, Keith E. J</creatorcontrib><creatorcontrib>Leonard, Joshua N</creatorcontrib><title>Generation of novel metabolite-responsive transcription regulator biosensors</title><description>Disclosed are systems, components, and methods for sensing a ligand in a cell or a reaction mixture. The disclosed systems, components, and methods may include and/or utilize a fusion protein comprising a ligand-binding protein and a DNA-binding protein. The fusion protein binds the ligand of the ligand-binding protein and modulates expression of a reporter gene operably linked to a promoter that is engineered to include specific binding sites for the DNA-binding protein. The difference in expression of the reporter gene in the presence of the ligand versus expression of the reporter gene in the absence of the ligand can be correlated to the concentration of the ligand in a reaction mixture.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjUsKwkAMQGfjQtQ7jAco1Go9gOJn4U5dl7SkJTAmQxJ7fot4AFdv83hvHm4XZFRwEo7SR5YRU3yhQyuJHAtFy8JGI0ZXYOuU8ldWHN4JXDS2JIZsorYMsx6S4erHRVifT4_jtcAszRSCbpp587xvyn25q-vqUG3_cT5IjTdn</recordid><startdate>20200331</startdate><enddate>20200331</enddate><creator>Younger, Andrew K. D</creator><creator>Tyo, Keith E. J</creator><creator>Leonard, Joshua N</creator><scope>EVB</scope></search><sort><creationdate>20200331</creationdate><title>Generation of novel metabolite-responsive transcription regulator biosensors</title><author>Younger, Andrew K. D ; Tyo, Keith E. J ; Leonard, Joshua N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US10604552B23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Younger, Andrew K. D</creatorcontrib><creatorcontrib>Tyo, Keith E. J</creatorcontrib><creatorcontrib>Leonard, Joshua N</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Younger, Andrew K. D</au><au>Tyo, Keith E. J</au><au>Leonard, Joshua N</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Generation of novel metabolite-responsive transcription regulator biosensors</title><date>2020-03-31</date><risdate>2020</risdate><abstract>Disclosed are systems, components, and methods for sensing a ligand in a cell or a reaction mixture. The disclosed systems, components, and methods may include and/or utilize a fusion protein comprising a ligand-binding protein and a DNA-binding protein. The fusion protein binds the ligand of the ligand-binding protein and modulates expression of a reporter gene operably linked to a promoter that is engineered to include specific binding sites for the DNA-binding protein. The difference in expression of the reporter gene in the presence of the ligand versus expression of the reporter gene in the absence of the ligand can be correlated to the concentration of the ligand in a reaction mixture.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_epo_espacenet_US10604552B2 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Generation of novel metabolite-responsive transcription regulator biosensors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T07%3A18%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=Younger,%20Andrew%20K.%20D&rft.date=2020-03-31&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EUS10604552B2%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |