Immunochromatographic test strip and detection method using immunochromatography for detecting target in red blood cell-containing sample

Immunochromatographic test strip and detection method using immunochromatography for detecting target in red blood cell-containing sample

A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of us...

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Hauptverfasser: Yamamoto, Mitsuaki, Shimizu, Tomo, Yoshida, Mayumi, Kono, Keigo, Yokokawa, Shinya
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator Yamamoto, Mitsuaki
Shimizu, Tomo
Yoshida, Mayumi
Kono, Keigo
Yokokawa, Shinya
description A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventors reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventors surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions.
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Immunochromatographic test strip and detection method using immunochromatography for detecting target in red blood cell-containing sample
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