Method for producing lactones from a strain of Aureobasidium pullulans
The invention relates to a method for producing lactones from a strain of Aureobasidium pullulans that are produced from a pre-culture of the Aureobasidium pullulans strain CBS 771.97, obtained from CBS-KNAW Fungal Biodiversity Center, Uppsalalaan 8, 3584 CT Utrecht, Netherlands, or related strains;...
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| creator | Courvoisier-Dezord, Elise Alphand, Véronique Lavoine-Hanneguelle, Sophie Archelas, Alain Boukhris-Uzan, Eva |
| description | The invention relates to a method for producing lactones from a strain of Aureobasidium pullulans that are produced from a pre-culture of the Aureobasidium pullulans strain CBS 771.97, obtained from CBS-KNAW Fungal Biodiversity Center, Uppsalalaan 8, 3584 CT Utrecht, Netherlands, or related strains; from an inoculum obtained from the pre-culture; a culture is produced by fermentation at a temperature between 20° C. and 40° C. over a period of at least 3 days so as to produce metabolites, in a sterilized aqueous production medium containing: a carbon source; a nitrogen source; a mineral salt solution; and a calcium source, at a concentration between 2 and 100 mM; and following the fermentation period, converting the metabolites produced into a lactone mixture comprising (R)-5,6-dihydro-6-pentyl-2H-pyran-2-one (or (R)-(−)-massoia lactone) and/or (4R,6R)-4-hydroxy-6-pentyl-tetrahydro-2H-pyran-2-one. |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING SPIRITS VINEGAR WINE |
| title | Method for producing lactones from a strain of Aureobasidium pullulans |
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