METHOD FOR STABILIZATION OF RECOMBINANT STRAIN OF ESCHERICHIA COLI PRODUCING MYCOPLASMA HOMINIS ARGININE DEIMINASE
The present invention relates to medical biotechnology and can be used as a way for stabilization of the recombinant strain of Escherichia coli which produces Mycoplasma hominis arginine deiminase (ADI) used for treatment of certain types of cancer. As a result of optimization of medium used for sel...
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| creator | FAIURA LIUBOV ROMANIVNA SYBIRNYI ANDRII ANDRIIOVYCH PYNIAHA YURII VOLODYMYROVYCH BORETSKYI YURII ROMANOVYCH |
| description | The present invention relates to medical biotechnology and can be used as a way for stabilization of the recombinant strain of Escherichia coli which produces Mycoplasma hominis arginine deiminase (ADI) used for treatment of certain types of cancer. As a result of optimization of medium used for selection of transformants, storage and inoculum preparation (LB with 0,2 % of arginine and 0,5 % of glucose) stability of producer was increased significantly that reduced the cost of the enzyme production substantially.
Способ стабилизации рекомбинантного штамма бактерий Escherichia coli по признаку "продукция аргининдезиминазы Mycoplasma hominis" предусматривает, что для селекции трансформантов, их хранения и выращивания стартовой культуры продуцента АДИ используется среду LB с добавлением 0,2 % аргинина и 0,5 % глюкозы.
Спосіб стабілізації рекомбінантного штаму бактерій Escherichia coli за ознакою "продукція аргініндезімінази Mycoplasma hominis", причому для селекції трансформантів, їх зберігання та вирощування стартової культури продуцента АДI використовується LB середовище з додатком 0,2 % аргініну і 0,5 % глюкози. |
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Способ стабилизации рекомбинантного штамма бактерий Escherichia coli по признаку "продукция аргининдезиминазы Mycoplasma hominis" предусматривает, что для селекции трансформантов, их хранения и выращивания стартовой культуры продуцента АДИ используется среду LB с добавлением 0,2 % аргинина и 0,5 % глюкозы.
Спосіб стабілізації рекомбінантного штаму бактерій Escherichia coli за ознакою "продукція аргініндезімінази Mycoplasma hominis", причому для селекції трансформантів, їх зберігання та вирощування стартової культури продуцента АДI використовується LB середовище з додатком 0,2 % аргініну і 0,5 % глюкози.</description><language>eng ; rus ; ukr</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2014</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20140127&DB=EPODOC&CC=UA&NR=87054U$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76419</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20140127&DB=EPODOC&CC=UA&NR=87054U$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>FAIURA LIUBOV ROMANIVNA</creatorcontrib><creatorcontrib>SYBIRNYI ANDRII ANDRIIOVYCH</creatorcontrib><creatorcontrib>PYNIAHA YURII VOLODYMYROVYCH</creatorcontrib><creatorcontrib>BORETSKYI YURII ROMANOVYCH</creatorcontrib><title>METHOD FOR STABILIZATION OF RECOMBINANT STRAIN OF ESCHERICHIA COLI PRODUCING MYCOPLASMA HOMINIS ARGININE DEIMINASE</title><description>The present invention relates to medical biotechnology and can be used as a way for stabilization of the recombinant strain of Escherichia coli which produces Mycoplasma hominis arginine deiminase (ADI) used for treatment of certain types of cancer. As a result of optimization of medium used for selection of transformants, storage and inoculum preparation (LB with 0,2 % of arginine and 0,5 % of glucose) stability of producer was increased significantly that reduced the cost of the enzyme production substantially.
Способ стабилизации рекомбинантного штамма бактерий Escherichia coli по признаку "продукция аргининдезиминазы Mycoplasma hominis" предусматривает, что для селекции трансформантов, их хранения и выращивания стартовой культуры продуцента АДИ используется среду LB с добавлением 0,2 % аргинина и 0,5 % глюкозы.
Спосіб стабілізації рекомбінантного штаму бактерій Escherichia coli за ознакою "продукція аргініндезімінази Mycoplasma hominis", причому для селекції трансформантів, їх зберігання та вирощування стартової культури продуцента АДI використовується LB середовище з додатком 0,2 % аргініну і 0,5 % глюкози.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2014</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqFirsKwkAQANNYiPoLsj8gCCrabu42uYXcbbhHoU0Iclaiwfj_GMTeamBm5sXLUjSioRIPIWLJDV8wsjiQCjwpsSU7dHGKHvlrKShDnpVhBCUNQ-tFJ8WuBntW0jYYLIIRy44DoK8nOgJNPBkMtCxmt_4-5tWPi2JdUVRmk4dnl8ehv-ZHfncJT8ftYZ_S7u_wARw_N8Q</recordid><startdate>20140127</startdate><enddate>20140127</enddate><creator>FAIURA LIUBOV ROMANIVNA</creator><creator>SYBIRNYI ANDRII ANDRIIOVYCH</creator><creator>PYNIAHA YURII VOLODYMYROVYCH</creator><creator>BORETSKYI YURII ROMANOVYCH</creator><scope>EVB</scope></search><sort><creationdate>20140127</creationdate><title>METHOD FOR STABILIZATION OF RECOMBINANT STRAIN OF ESCHERICHIA COLI PRODUCING MYCOPLASMA HOMINIS ARGININE DEIMINASE</title><author>FAIURA LIUBOV ROMANIVNA ; SYBIRNYI ANDRII ANDRIIOVYCH ; PYNIAHA YURII VOLODYMYROVYCH ; BORETSKYI YURII ROMANOVYCH</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_UA87054UU3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rus ; ukr</language><creationdate>2014</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>FAIURA LIUBOV ROMANIVNA</creatorcontrib><creatorcontrib>SYBIRNYI ANDRII ANDRIIOVYCH</creatorcontrib><creatorcontrib>PYNIAHA YURII VOLODYMYROVYCH</creatorcontrib><creatorcontrib>BORETSKYI YURII ROMANOVYCH</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>FAIURA LIUBOV ROMANIVNA</au><au>SYBIRNYI ANDRII ANDRIIOVYCH</au><au>PYNIAHA YURII VOLODYMYROVYCH</au><au>BORETSKYI YURII ROMANOVYCH</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR STABILIZATION OF RECOMBINANT STRAIN OF ESCHERICHIA COLI PRODUCING MYCOPLASMA HOMINIS ARGININE DEIMINASE</title><date>2014-01-27</date><risdate>2014</risdate><abstract>The present invention relates to medical biotechnology and can be used as a way for stabilization of the recombinant strain of Escherichia coli which produces Mycoplasma hominis arginine deiminase (ADI) used for treatment of certain types of cancer. As a result of optimization of medium used for selection of transformants, storage and inoculum preparation (LB with 0,2 % of arginine and 0,5 % of glucose) stability of producer was increased significantly that reduced the cost of the enzyme production substantially.
Способ стабилизации рекомбинантного штамма бактерий Escherichia coli по признаку "продукция аргининдезиминазы Mycoplasma hominis" предусматривает, что для селекции трансформантов, их хранения и выращивания стартовой культуры продуцента АДИ используется среду LB с добавлением 0,2 % аргинина и 0,5 % глюкозы.
Спосіб стабілізації рекомбінантного штаму бактерій Escherichia coli за ознакою "продукція аргініндезімінази Mycoplasma hominis", причому для селекції трансформантів, їх зберігання та вирощування стартової культури продуцента АДI використовується LB середовище з додатком 0,2 % аргініну і 0,5 % глюкози.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD FOR STABILIZATION OF RECOMBINANT STRAIN OF ESCHERICHIA COLI PRODUCING MYCOPLASMA HOMINIS ARGININE DEIMINASE |
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