METHOD FOR OBTAINING DNA FROM BIOLOGICAL OBJECTS
A method for obtaining DNA from biological objects includes destruction of cells, simultaneous lysis of cells, extraction of DNA specimens and cleaning thereof of cellular debris, which is performed by a complex action of chloroform and buffer, which is composed of sucrose, triton X-100, Tris-HCl (p...
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| creator | BRUIAKA OLHA PETRIVNA ALKHIMOVA OLENA HEORHIIVNA MARTYNENKO OLENA IVANIVNA KYRYLENKO TAMARA KOSTIANTYNIVNA BRUIAKA PETRO PYLYPOVYCH |
| description | A method for obtaining DNA from biological objects includes destruction of cells, simultaneous lysis of cells, extraction of DNA specimens and cleaning thereof of cellular debris, which is performed by a complex action of chloroform and buffer, which is composed of sucrose, triton X-100, Tris-HCl (pH 7.5-8), EDTA and water. The lysis of cellular wall, extraction and DNA cleaning of cellular debris are carried out in one stage for not less one hour at the temperature of +20 DEGREE C...+70 DEGREE C and buffer, which is composed in addition of sodium acetate and complex of non-ionic surface active substances with hydrated peroxide. |
| format | Patent |
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The lysis of cellular wall, extraction and DNA cleaning of cellular debris are carried out in one stage for not less one hour at the temperature of +20 DEGREE C...+70 DEGREE C and buffer, which is composed in addition of sodium acetate and complex of non-ionic surface active substances with hydrated peroxide.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DERIVATIVES THEREOF ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NUCLEIC ACIDS ; NUCLEOSIDES ; NUCLEOTIDES ; ORGANIC CHEMISTRY ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; SUGARS ; VINEGAR ; WINE</subject><creationdate>2006</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20060717&DB=EPODOC&CC=UA&NR=16192U$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20060717&DB=EPODOC&CC=UA&NR=16192U$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>BRUIAKA OLHA PETRIVNA</creatorcontrib><creatorcontrib>ALKHIMOVA OLENA HEORHIIVNA</creatorcontrib><creatorcontrib>MARTYNENKO OLENA IVANIVNA</creatorcontrib><creatorcontrib>KYRYLENKO TAMARA KOSTIANTYNIVNA</creatorcontrib><creatorcontrib>BRUIAKA PETRO PYLYPOVYCH</creatorcontrib><title>METHOD FOR OBTAINING DNA FROM BIOLOGICAL OBJECTS</title><description>A method for obtaining DNA from biological objects includes destruction of cells, simultaneous lysis of cells, extraction of DNA specimens and cleaning thereof of cellular debris, which is performed by a complex action of chloroform and buffer, which is composed of sucrose, triton X-100, Tris-HCl (pH 7.5-8), EDTA and water. 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The lysis of cellular wall, extraction and DNA cleaning of cellular debris are carried out in one stage for not less one hour at the temperature of +20 DEGREE C...+70 DEGREE C and buffer, which is composed in addition of sodium acetate and complex of non-ionic surface active substances with hydrated peroxide.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE |
| title | METHOD FOR OBTAINING DNA FROM BIOLOGICAL OBJECTS |
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