Modified thermostable DNA polymerase

The invention provides thermostable DNA polymerase enzymes that comprises the amino acid sequence SerGlnIleXaaLeuArgXaa (SEQ ID NO: 1), wherein ""Xaa"" at position 4 of this sequence is any amino acid residue but not a glutamic acid residue (Glu), preferably a glycine residue and...

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Bibliographische Detailangaben
Hauptverfasser:	KALMAN, LISA VIVIAN, REICHERT, FRED LAWRENCE, GELFAND, DAVID HARROW
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides thermostable DNA polymerase enzymes that comprises the amino acid sequence SerGlnIleXaaLeuArgXaa (SEQ ID NO: 1), wherein ""Xaa"" at position 4 of this sequence is any amino acid residue but not a glutamic acid residue (Glu), preferably a glycine residue and ""Xaa"" at position 7 of this sequence is a valine residue (Val) or an isoleucine residue (Ile). The thermostable DNA polymerases of the invention have enhanced efficiency for incorporating unconventional nucleotides, such as ribonucleotides, into DNA products and are advantageous in many in vitro synthesis applications. Such enzymes are particularly useful for use in nucleic acid sequencing protocols and provide novel means for DNA sequence analysis with cost and efficiency advantages. Also claimed are nucleic acids encoding said polymerases, vectors and hoste cells comprising such a nucleic acid, as well as compositions for use in a DNA sequencing reaction, kits and methods for sequencing including such polymerases.