Method for the extraction of periplasmic proteins from prokaryotic microorganisms in the presence of arginine
The invention relates to the use of arginine as an agent for the extraction of recombinant periplasmic proteins. The invention further relates to a method for the extraction of a periplasmic protein of interest, which consists in: (i) suspending the pellet of cells or cell debris from cells originat...
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| creator | MALDONADO, PAUL SALOME, MARC LEGOUX, RICHARD |
| description | The invention relates to the use of arginine as an agent for the extraction of recombinant periplasmic proteins. The invention further relates to a method for the extraction of a periplasmic protein of interest, which consists in: (i) suspending the pellet of cells or cell debris from cells originating from the culture of a prokaryotic microorganism, transformed by means of an expression vector containing a gene coding for the said protein and means for its expression in the periplasm of the said microorganism, in a buffer solution containing arginine; and (ii) recovering the protein of interest in the supernatant of the bacterial suspension thereby obtained. |
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The invention further relates to a method for the extraction of a periplasmic protein of interest, which consists in: (i) suspending the pellet of cells or cell debris from cells originating from the culture of a prokaryotic microorganism, transformed by means of an expression vector containing a gene coding for the said protein and means for its expression in the periplasm of the said microorganism, in a buffer solution containing arginine; and (ii) recovering the protein of interest in the supernatant of the bacterial suspension thereby obtained.</description><edition>7</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DERIVATIVES THEREOF ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NUCLEIC ACIDS ; NUCLEOSIDES ; NUCLEOTIDES ; ORGANIC CHEMISTRY ; PEPTIDES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; SUGARS ; VINEGAR ; WINE</subject><creationdate>2000</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20000901&DB=EPODOC&CC=TW&NR=403760B$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20000901&DB=EPODOC&CC=TW&NR=403760B$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MALDONADO, PAUL</creatorcontrib><creatorcontrib>SALOME, MARC</creatorcontrib><creatorcontrib>LEGOUX, RICHARD</creatorcontrib><title>Method for the extraction of periplasmic proteins from prokaryotic microorganisms in the presence of arginine</title><description>The invention relates to the use of arginine as an agent for the extraction of recombinant periplasmic proteins. The invention further relates to a method for the extraction of a periplasmic protein of interest, which consists in: (i) suspending the pellet of cells or cell debris from cells originating from the culture of a prokaryotic microorganism, transformed by means of an expression vector containing a gene coding for the said protein and means for its expression in the periplasm of the said microorganism, in a buffer solution containing arginine; and (ii) recovering the protein of interest in the supernatant of the bacterial suspension thereby obtained.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>DERIVATIVES THEREOF</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOSIDES</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>SUGARS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2000</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqFjDEKwkAQRbexEPUMzgWEQET7iGJjF7AMyzqbDGZnltkp9PYmYm_1-bzHW7p0QxvkAVEUbEDAl6kPRsIgETIq5dGXRAGyiiFxgaiS5vf0-habyERVRHvPVFIB4m8pKxbkgHPHa09MjGu3iH4suPntym0v5_Z03WGWDkv2ARmta-_7qj4eqqap_xsf6MpChQ</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>MALDONADO, PAUL</creator><creator>SALOME, MARC</creator><creator>LEGOUX, RICHARD</creator><scope>EVB</scope></search><sort><creationdate>20000901</creationdate><title>Method for the extraction of periplasmic proteins from prokaryotic microorganisms in the presence of arginine</title><author>MALDONADO, PAUL ; SALOME, MARC ; LEGOUX, RICHARD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_TW403760BB3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2000</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>DERIVATIVES THEREOF</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOSIDES</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>SUGARS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>MALDONADO, PAUL</creatorcontrib><creatorcontrib>SALOME, MARC</creatorcontrib><creatorcontrib>LEGOUX, RICHARD</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MALDONADO, PAUL</au><au>SALOME, MARC</au><au>LEGOUX, RICHARD</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for the extraction of periplasmic proteins from prokaryotic microorganisms in the presence of arginine</title><date>2000-09-01</date><risdate>2000</risdate><abstract>The invention relates to the use of arginine as an agent for the extraction of recombinant periplasmic proteins. The invention further relates to a method for the extraction of a periplasmic protein of interest, which consists in: (i) suspending the pellet of cells or cell debris from cells originating from the culture of a prokaryotic microorganism, transformed by means of an expression vector containing a gene coding for the said protein and means for its expression in the periplasm of the said microorganism, in a buffer solution containing arginine; and (ii) recovering the protein of interest in the supernatant of the bacterial suspension thereby obtained.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE |
| title | Method for the extraction of periplasmic proteins from prokaryotic microorganisms in the presence of arginine |
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