Method for separating and detecting exosomes, and kit for separation and detection thereof

This method for separating and detecting exosomes comprises: a complex formation step for contacting a biological sample with a capture molecule, said capture molecule containing a substance specifically binding to an antigen expressed on cancer cell surface, to form an exosome-capture molecule comp...

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Hauptverfasser: YAGUCHI, TOMONORI, WAKUI, SEIKI, KAWAKAMI, YUTAKA
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creator YAGUCHI, TOMONORI
WAKUI, SEIKI
KAWAKAMI, YUTAKA
description This method for separating and detecting exosomes comprises: a complex formation step for contacting a biological sample with a capture molecule, said capture molecule containing a substance specifically binding to an antigen expressed on cancer cell surface, to form an exosome-capture molecule complex; and a detection step for contacting the complex with a detection molecule, said detection molecule containing a substance specifically binding to an antigen expressed on cancer cell surface and a labeling substance, and then detecting the complex with the use of the detection molecule, wherein, in the capture molecule and/or the detection molecule, the antigen expressed on cancer cell surface is cell surface vimentin.
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language chi ; eng
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subjects APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title Method for separating and detecting exosomes, and kit for separation and detection thereof
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