METHOD FOR MODIFYING THE GLASS SURFACE FOR THE INTRODUCTION OF FUNCTIONAL AMINO GROUPS
FIELD: biotechnology equipment.SUBSTANCE: invention can be used in the production of materials for the construction of bio- and chemical sensors in the field of gene diagnostics to obtain gene libraries and conduct a polymerase chain reaction on nucleic acids immobilized on the surface of glass. The...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; rus |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | ZHUKOV Mikhail Valerevich NIKOLAEVA Mariya Evgenevna NECHAEV Andrej Valerevich ALEKSEEV Yakov Igorevich GOLUBOK Aleksandr Olegovich |
| description | FIELD: biotechnology equipment.SUBSTANCE: invention can be used in the production of materials for the construction of bio- and chemical sensors in the field of gene diagnostics to obtain gene libraries and conduct a polymerase chain reaction on nucleic acids immobilized on the surface of glass. The method for modifying the glass surface for the introduction of functional amino groups includes activation with a solution of concentrated H2SO4and 30% H2O2, washing with distilled water, drying, treatment with a solution of tetraethoxysilane (TEOS) in a nonpolar organic solvent in the presence of an aqueous solution of ammonia and nonylphenoxypolyethoxyethanol, washing with a nonpolar organic solvent and ethanol, drying. Then the treatment is carried out with a solution (3-aminopropyl)trialkoxysilane in ethanol, washing with ethanol, drying. Cyclohexane is used as a non-polar organic solvent.EFFECT: invention makes it possible to obtain a glass surface with a controlled density and uniform distribution of functional amino groups for subsequent immobilization of oligonucleotides by means of an amide bond.6 cl, 5 dwg, 5 ex
Изобретение может быть использовано при получении материалов для конструирования био- и химических сенсоров в области генной диагностики для получения генных библиотек и проведения полимеразной цепной реакции на нуклеиновых кислотах, иммобилизованных на поверхности стекла. Способ модификации поверхности стекла для введения функциональных аминогрупп включает активацию раствором концентрированной H2SO4и 30% H2O2, промывку дистиллированной водой, сушку, обработку раствором тетраэтоксисилана (TEOS) в неполярном органическом растворителе в присутствии водного раствора аммиака и нонилфеноксиполиэтоксиэтанола, промывку неполярным органическим растворителем и этанолом, сушку. Затем проводят обработку раствором (3-аминопропил)триалкоксисилана в этаноле, промывку этанолом, сушку. В качестве неполярного органического растворителя используют циклогексан. Изобретение позволяет получать поверхность стекла с контролируемой плотностью и равномерным распределением функциональных аминогрупп для последующей иммобилизации олигонуклеотидов посредством амидной связи. 5 з.п. ф-лы, 5 ил., 5 пр. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_RU2762108C2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>RU2762108C2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_RU2762108C23</originalsourceid><addsrcrecordid>eNrjZAjzdQ3x8HdRcPMPUvD1d_F0i_T0c1cI8XBVcPdxDA5WCA4NcnN0dgXLg0Q9_UKC_F1CnUM8_f0U_N0U3EL9wGxHHwVHX08_fwX3IP_QgGAeBta0xJziVF4ozc2g4OYa4uyhm1qQH59aXJCYnJqXWhIfFGpkbmZkaGDhbGRMhBIAMq4wew</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>METHOD FOR MODIFYING THE GLASS SURFACE FOR THE INTRODUCTION OF FUNCTIONAL AMINO GROUPS</title><source>esp@cenet</source><creator>ZHUKOV Mikhail Valerevich ; NIKOLAEVA Mariya Evgenevna ; NECHAEV Andrej Valerevich ; ALEKSEEV Yakov Igorevich ; GOLUBOK Aleksandr Olegovich</creator><creatorcontrib>ZHUKOV Mikhail Valerevich ; NIKOLAEVA Mariya Evgenevna ; NECHAEV Andrej Valerevich ; ALEKSEEV Yakov Igorevich ; GOLUBOK Aleksandr Olegovich</creatorcontrib><description>FIELD: biotechnology equipment.SUBSTANCE: invention can be used in the production of materials for the construction of bio- and chemical sensors in the field of gene diagnostics to obtain gene libraries and conduct a polymerase chain reaction on nucleic acids immobilized on the surface of glass. The method for modifying the glass surface for the introduction of functional amino groups includes activation with a solution of concentrated H2SO4and 30% H2O2, washing with distilled water, drying, treatment with a solution of tetraethoxysilane (TEOS) in a nonpolar organic solvent in the presence of an aqueous solution of ammonia and nonylphenoxypolyethoxyethanol, washing with a nonpolar organic solvent and ethanol, drying. Then the treatment is carried out with a solution (3-aminopropyl)trialkoxysilane in ethanol, washing with ethanol, drying. Cyclohexane is used as a non-polar organic solvent.EFFECT: invention makes it possible to obtain a glass surface with a controlled density and uniform distribution of functional amino groups for subsequent immobilization of oligonucleotides by means of an amide bond.6 cl, 5 dwg, 5 ex
Изобретение может быть использовано при получении материалов для конструирования био- и химических сенсоров в области генной диагностики для получения генных библиотек и проведения полимеразной цепной реакции на нуклеиновых кислотах, иммобилизованных на поверхности стекла. Способ модификации поверхности стекла для введения функциональных аминогрупп включает активацию раствором концентрированной H2SO4и 30% H2O2, промывку дистиллированной водой, сушку, обработку раствором тетраэтоксисилана (TEOS) в неполярном органическом растворителе в присутствии водного раствора аммиака и нонилфеноксиполиэтоксиэтанола, промывку неполярным органическим растворителем и этанолом, сушку. Затем проводят обработку раствором (3-аминопропил)триалкоксисилана в этаноле, промывку этанолом, сушку. В качестве неполярного органического растворителя используют циклогексан. Изобретение позволяет получать поверхность стекла с контролируемой плотностью и равномерным распределением функциональных аминогрупп для последующей иммобилизации олигонуклеотидов посредством амидной связи. 5 з.п. ф-лы, 5 ил., 5 пр.</description><language>eng ; rus</language><subject>ADHESIVES ; BEER ; BIOCHEMISTRY ; CHEMICAL COMPOSITION OF GLASSES, GLAZES, OR VITREOUSENAMELS ; CHEMICAL PAINT OR INK REMOVERS ; CHEMISTRY ; COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS ; COMPOSITIONS THEREOF ; CORRECTING FLUIDS ; CULTURE MEDIA ; DYES ; ENZYMOLOGY ; FILLING PASTES ; GLASS ; INKS ; JOINING GLASS TO GLASS OR OTHER MATERIALS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MINERAL OR SLAG WOOL ; MISCELLANEOUS APPLICATIONS OF MATERIALS ; MISCELLANEOUS COMPOSITIONS ; MUTATION OR GENETIC ENGINEERING ; NATURAL RESINS ; PAINTS ; PASTES OR SOLIDS FOR COLOURING OR PRINTING ; POLISHES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS,MINERALS OR SLAGS ; SURFACE TREATMENT OF GLASS ; USE OF MATERIALS THEREFOR ; VINEGAR ; WINE ; WOODSTAINS</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211215&DB=EPODOC&CC=RU&NR=2762108C2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211215&DB=EPODOC&CC=RU&NR=2762108C2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHUKOV Mikhail Valerevich</creatorcontrib><creatorcontrib>NIKOLAEVA Mariya Evgenevna</creatorcontrib><creatorcontrib>NECHAEV Andrej Valerevich</creatorcontrib><creatorcontrib>ALEKSEEV Yakov Igorevich</creatorcontrib><creatorcontrib>GOLUBOK Aleksandr Olegovich</creatorcontrib><title>METHOD FOR MODIFYING THE GLASS SURFACE FOR THE INTRODUCTION OF FUNCTIONAL AMINO GROUPS</title><description>FIELD: biotechnology equipment.SUBSTANCE: invention can be used in the production of materials for the construction of bio- and chemical sensors in the field of gene diagnostics to obtain gene libraries and conduct a polymerase chain reaction on nucleic acids immobilized on the surface of glass. The method for modifying the glass surface for the introduction of functional amino groups includes activation with a solution of concentrated H2SO4and 30% H2O2, washing with distilled water, drying, treatment with a solution of tetraethoxysilane (TEOS) in a nonpolar organic solvent in the presence of an aqueous solution of ammonia and nonylphenoxypolyethoxyethanol, washing with a nonpolar organic solvent and ethanol, drying. Then the treatment is carried out with a solution (3-aminopropyl)trialkoxysilane in ethanol, washing with ethanol, drying. Cyclohexane is used as a non-polar organic solvent.EFFECT: invention makes it possible to obtain a glass surface with a controlled density and uniform distribution of functional amino groups for subsequent immobilization of oligonucleotides by means of an amide bond.6 cl, 5 dwg, 5 ex
Изобретение может быть использовано при получении материалов для конструирования био- и химических сенсоров в области генной диагностики для получения генных библиотек и проведения полимеразной цепной реакции на нуклеиновых кислотах, иммобилизованных на поверхности стекла. Способ модификации поверхности стекла для введения функциональных аминогрупп включает активацию раствором концентрированной H2SO4и 30% H2O2, промывку дистиллированной водой, сушку, обработку раствором тетраэтоксисилана (TEOS) в неполярном органическом растворителе в присутствии водного раствора аммиака и нонилфеноксиполиэтоксиэтанола, промывку неполярным органическим растворителем и этанолом, сушку. Затем проводят обработку раствором (3-аминопропил)триалкоксисилана в этаноле, промывку этанолом, сушку. В качестве неполярного органического растворителя используют циклогексан. Изобретение позволяет получать поверхность стекла с контролируемой плотностью и равномерным распределением функциональных аминогрупп для последующей иммобилизации олигонуклеотидов посредством амидной связи. 5 з.п. ф-лы, 5 ил., 5 пр.</description><subject>ADHESIVES</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMICAL COMPOSITION OF GLASSES, GLAZES, OR VITREOUSENAMELS</subject><subject>CHEMICAL PAINT OR INK REMOVERS</subject><subject>CHEMISTRY</subject><subject>COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS</subject><subject>COMPOSITIONS THEREOF</subject><subject>CORRECTING FLUIDS</subject><subject>CULTURE MEDIA</subject><subject>DYES</subject><subject>ENZYMOLOGY</subject><subject>FILLING PASTES</subject><subject>GLASS</subject><subject>INKS</subject><subject>JOINING GLASS TO GLASS OR OTHER MATERIALS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MINERAL OR SLAG WOOL</subject><subject>MISCELLANEOUS APPLICATIONS OF MATERIALS</subject><subject>MISCELLANEOUS COMPOSITIONS</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NATURAL RESINS</subject><subject>PAINTS</subject><subject>PASTES OR SOLIDS FOR COLOURING OR PRINTING</subject><subject>POLISHES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS,MINERALS OR SLAGS</subject><subject>SURFACE TREATMENT OF GLASS</subject><subject>USE OF MATERIALS THEREFOR</subject><subject>VINEGAR</subject><subject>WINE</subject><subject>WOODSTAINS</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZAjzdQ3x8HdRcPMPUvD1d_F0i_T0c1cI8XBVcPdxDA5WCA4NcnN0dgXLg0Q9_UKC_F1CnUM8_f0U_N0U3EL9wGxHHwVHX08_fwX3IP_QgGAeBta0xJziVF4ozc2g4OYa4uyhm1qQH59aXJCYnJqXWhIfFGpkbmZkaGDhbGRMhBIAMq4wew</recordid><startdate>20211215</startdate><enddate>20211215</enddate><creator>ZHUKOV Mikhail Valerevich</creator><creator>NIKOLAEVA Mariya Evgenevna</creator><creator>NECHAEV Andrej Valerevich</creator><creator>ALEKSEEV Yakov Igorevich</creator><creator>GOLUBOK Aleksandr Olegovich</creator><scope>EVB</scope></search><sort><creationdate>20211215</creationdate><title>METHOD FOR MODIFYING THE GLASS SURFACE FOR THE INTRODUCTION OF FUNCTIONAL AMINO GROUPS</title><author>ZHUKOV Mikhail Valerevich ; NIKOLAEVA Mariya Evgenevna ; NECHAEV Andrej Valerevich ; ALEKSEEV Yakov Igorevich ; GOLUBOK Aleksandr Olegovich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_RU2762108C23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rus</language><creationdate>2021</creationdate><topic>ADHESIVES</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMICAL COMPOSITION OF GLASSES, GLAZES, OR VITREOUSENAMELS</topic><topic>CHEMICAL PAINT OR INK REMOVERS</topic><topic>CHEMISTRY</topic><topic>COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS</topic><topic>COMPOSITIONS THEREOF</topic><topic>CORRECTING FLUIDS</topic><topic>CULTURE MEDIA</topic><topic>DYES</topic><topic>ENZYMOLOGY</topic><topic>FILLING PASTES</topic><topic>GLASS</topic><topic>INKS</topic><topic>JOINING GLASS TO GLASS OR OTHER MATERIALS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MINERAL OR SLAG WOOL</topic><topic>MISCELLANEOUS APPLICATIONS OF MATERIALS</topic><topic>MISCELLANEOUS COMPOSITIONS</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NATURAL RESINS</topic><topic>PAINTS</topic><topic>PASTES OR SOLIDS FOR COLOURING OR PRINTING</topic><topic>POLISHES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS,MINERALS OR SLAGS</topic><topic>SURFACE TREATMENT OF GLASS</topic><topic>USE OF MATERIALS THEREFOR</topic><topic>VINEGAR</topic><topic>WINE</topic><topic>WOODSTAINS</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHUKOV Mikhail Valerevich</creatorcontrib><creatorcontrib>NIKOLAEVA Mariya Evgenevna</creatorcontrib><creatorcontrib>NECHAEV Andrej Valerevich</creatorcontrib><creatorcontrib>ALEKSEEV Yakov Igorevich</creatorcontrib><creatorcontrib>GOLUBOK Aleksandr Olegovich</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHUKOV Mikhail Valerevich</au><au>NIKOLAEVA Mariya Evgenevna</au><au>NECHAEV Andrej Valerevich</au><au>ALEKSEEV Yakov Igorevich</au><au>GOLUBOK Aleksandr Olegovich</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR MODIFYING THE GLASS SURFACE FOR THE INTRODUCTION OF FUNCTIONAL AMINO GROUPS</title><date>2021-12-15</date><risdate>2021</risdate><abstract>FIELD: biotechnology equipment.SUBSTANCE: invention can be used in the production of materials for the construction of bio- and chemical sensors in the field of gene diagnostics to obtain gene libraries and conduct a polymerase chain reaction on nucleic acids immobilized on the surface of glass. The method for modifying the glass surface for the introduction of functional amino groups includes activation with a solution of concentrated H2SO4and 30% H2O2, washing with distilled water, drying, treatment with a solution of tetraethoxysilane (TEOS) in a nonpolar organic solvent in the presence of an aqueous solution of ammonia and nonylphenoxypolyethoxyethanol, washing with a nonpolar organic solvent and ethanol, drying. Then the treatment is carried out with a solution (3-aminopropyl)trialkoxysilane in ethanol, washing with ethanol, drying. Cyclohexane is used as a non-polar organic solvent.EFFECT: invention makes it possible to obtain a glass surface with a controlled density and uniform distribution of functional amino groups for subsequent immobilization of oligonucleotides by means of an amide bond.6 cl, 5 dwg, 5 ex
Изобретение может быть использовано при получении материалов для конструирования био- и химических сенсоров в области генной диагностики для получения генных библиотек и проведения полимеразной цепной реакции на нуклеиновых кислотах, иммобилизованных на поверхности стекла. Способ модификации поверхности стекла для введения функциональных аминогрупп включает активацию раствором концентрированной H2SO4и 30% H2O2, промывку дистиллированной водой, сушку, обработку раствором тетраэтоксисилана (TEOS) в неполярном органическом растворителе в присутствии водного раствора аммиака и нонилфеноксиполиэтоксиэтанола, промывку неполярным органическим растворителем и этанолом, сушку. Затем проводят обработку раствором (3-аминопропил)триалкоксисилана в этаноле, промывку этанолом, сушку. В качестве неполярного органического растворителя используют циклогексан. Изобретение позволяет получать поверхность стекла с контролируемой плотностью и равномерным распределением функциональных аминогрупп для последующей иммобилизации олигонуклеотидов посредством амидной связи. 5 з.п. ф-лы, 5 ил., 5 пр.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; rus |
| recordid | cdi_epo_espacenet_RU2762108C2 |
| source | esp@cenet |
| subjects | ADHESIVES BEER BIOCHEMISTRY CHEMICAL COMPOSITION OF GLASSES, GLAZES, OR VITREOUSENAMELS CHEMICAL PAINT OR INK REMOVERS CHEMISTRY COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS COMPOSITIONS THEREOF CORRECTING FLUIDS CULTURE MEDIA DYES ENZYMOLOGY FILLING PASTES GLASS INKS JOINING GLASS TO GLASS OR OTHER MATERIALS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MINERAL OR SLAG WOOL MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MUTATION OR GENETIC ENGINEERING NATURAL RESINS PAINTS PASTES OR SOLIDS FOR COLOURING OR PRINTING POLISHES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS,MINERALS OR SLAGS SURFACE TREATMENT OF GLASS USE OF MATERIALS THEREFOR VINEGAR WINE WOODSTAINS |
| title | METHOD FOR MODIFYING THE GLASS SURFACE FOR THE INTRODUCTION OF FUNCTIONAL AMINO GROUPS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T18%3A35%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=ZHUKOV%20Mikhail%20Valerevich&rft.date=2021-12-15&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ERU2762108C2%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |