FLOW-THROUGH CELLS WITH HYDROGEL COATING
FIELD: genetics.SUBSTANCE: invention relates to biological matrices applicable for genetic sequencing and for detection and research of various molecules, families of molecules, levels of gene expression, single nucleotide polymorphisms, and for genotyping. Described is a flow-through cell containin...
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| creator | ZHEN, Khuntszi BOUTELL, Dzhonatan Mark BOUEN, M. Shejn MUN, Dzhon A SKINNER, Gari Mark CHEN, Kenni NEMIROSKI, Aleks |
| description | FIELD: genetics.SUBSTANCE: invention relates to biological matrices applicable for genetic sequencing and for detection and research of various molecules, families of molecules, levels of gene expression, single nucleotide polymorphisms, and for genotyping. Described is a flow-through cell containing a structured substrate including recesses. A layer of functionalised coating is applied to the recesses of the structured substrate of the flow-through cell. The recesses are separated from each other by intermediate sections. The primer is grafted to the layer of the functionalised coating resulting in a grafted layer of the functionalised coating located in the recesses. The hydrogel is applied to at least the grafted layer of the functionalised coating.EFFECT: technical result is improved formation of monoclonal clusters and purity of the main component of the cluster in wells with polyclonal clusters which additionally increases sequencing yield and data quality.19 cl, 8 dwg, 2 ex
Изобретение относится к биологическим матрицам, которые могут быть применены для проведения генетического секвенирования, а также для обнаружения и исследования разнообразных молекул, семейств молекул, уровней экспрессии генов, однонуклеотидных полиморфизмов и для проведения генотипирования. Описана проточная ячейка, содержащая структуированную подложку, включающую углубления. Слой функционализированного покрытия наносят на углубления структурированной подложки проточной ячейки. Углубления отделены друг от друга промежуточными участками. Праймер привит к слою функционализированного покрытия, в результате чего образуется привитой слой функционализированного покрытия, находящегося в углублениях. Гидрогель наносят на по меньшей мере привитой слой функционализированного покрытия. Технический результат - улучшение образования моноклональных кластеров и чистоты основного компонента кластера в лунках с поликлональными кластерами, что также повышает выход секвенирования и качество данных. 3 н. и 16 з.п. ф-лы, 8 ил., 2 пр. |
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| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_RU2751517C2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>RU2751517C2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_RU2751517C23</originalsourceid><addsrcrecordid>eNrjZNBw8_EP1w3xCPIPdfdQcHb18QlWCPcM8VDwiHQJ8nd39VFw9ncM8fRz52FgTUvMKU7lhdLcDApuriHOHrqpBfnxqcUFicmpeakl8UGhRuamhqaG5s5GxkQoAQDSICPx</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>FLOW-THROUGH CELLS WITH HYDROGEL COATING</title><source>esp@cenet</source><creator>ZHEN, Khuntszi ; BOUTELL, Dzhonatan Mark ; BOUEN, M. Shejn ; MUN, Dzhon A ; SKINNER, Gari Mark ; CHEN, Kenni ; NEMIROSKI, Aleks</creator><creatorcontrib>ZHEN, Khuntszi ; BOUTELL, Dzhonatan Mark ; BOUEN, M. Shejn ; MUN, Dzhon A ; SKINNER, Gari Mark ; CHEN, Kenni ; NEMIROSKI, Aleks</creatorcontrib><description>FIELD: genetics.SUBSTANCE: invention relates to biological matrices applicable for genetic sequencing and for detection and research of various molecules, families of molecules, levels of gene expression, single nucleotide polymorphisms, and for genotyping. Described is a flow-through cell containing a structured substrate including recesses. A layer of functionalised coating is applied to the recesses of the structured substrate of the flow-through cell. The recesses are separated from each other by intermediate sections. The primer is grafted to the layer of the functionalised coating resulting in a grafted layer of the functionalised coating located in the recesses. The hydrogel is applied to at least the grafted layer of the functionalised coating.EFFECT: technical result is improved formation of monoclonal clusters and purity of the main component of the cluster in wells with polyclonal clusters which additionally increases sequencing yield and data quality.19 cl, 8 dwg, 2 ex
Изобретение относится к биологическим матрицам, которые могут быть применены для проведения генетического секвенирования, а также для обнаружения и исследования разнообразных молекул, семейств молекул, уровней экспрессии генов, однонуклеотидных полиморфизмов и для проведения генотипирования. Описана проточная ячейка, содержащая структуированную подложку, включающую углубления. Слой функционализированного покрытия наносят на углубления структурированной подложки проточной ячейки. Углубления отделены друг от друга промежуточными участками. Праймер привит к слою функционализированного покрытия, в результате чего образуется привитой слой функционализированного покрытия, находящегося в углублениях. Гидрогель наносят на по меньшей мере привитой слой функционализированного покрытия. Технический результат - улучшение образования моноклональных кластеров и чистоты основного компонента кластера в лунках с поликлональными кластерами, что также повышает выход секвенирования и качество данных. 3 н. и 16 з.п. ф-лы, 8 ил., 2 пр.</description><language>eng ; rus</language><subject>ADHESIVES ; BEER ; BIOCHEMISTRY ; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY ; CHEMICAL PAINT OR INK REMOVERS ; CHEMISTRY ; COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS ; COMPOSITIONS BASED THEREON ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CORRECTING FLUIDS ; DYES ; ENZYMOLOGY ; FILLING PASTES ; INKS ; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MISCELLANEOUS APPLICATIONS OF MATERIALS ; MISCELLANEOUS COMPOSITIONS ; MUTATION OR GENETIC ENGINEERING ; NATURAL RESINS ; ORGANIC MACROMOLECULAR COMPOUNDS ; PAINTS ; PASTES OR SOLIDS FOR COLOURING OR PRINTING ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; POLISHES ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; THEIR PREPARATION OR CHEMICAL WORKING-UP ; THEIR RELEVANT APPARATUS ; TRANSPORTING ; USE OF MATERIALS THEREFOR ; VINEGAR ; WINE ; WOODSTAINS</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210714&DB=EPODOC&CC=RU&NR=2751517C2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210714&DB=EPODOC&CC=RU&NR=2751517C2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHEN, Khuntszi</creatorcontrib><creatorcontrib>BOUTELL, Dzhonatan Mark</creatorcontrib><creatorcontrib>BOUEN, M. Shejn</creatorcontrib><creatorcontrib>MUN, Dzhon A</creatorcontrib><creatorcontrib>SKINNER, Gari Mark</creatorcontrib><creatorcontrib>CHEN, Kenni</creatorcontrib><creatorcontrib>NEMIROSKI, Aleks</creatorcontrib><title>FLOW-THROUGH CELLS WITH HYDROGEL COATING</title><description>FIELD: genetics.SUBSTANCE: invention relates to biological matrices applicable for genetic sequencing and for detection and research of various molecules, families of molecules, levels of gene expression, single nucleotide polymorphisms, and for genotyping. Described is a flow-through cell containing a structured substrate including recesses. A layer of functionalised coating is applied to the recesses of the structured substrate of the flow-through cell. The recesses are separated from each other by intermediate sections. The primer is grafted to the layer of the functionalised coating resulting in a grafted layer of the functionalised coating located in the recesses. The hydrogel is applied to at least the grafted layer of the functionalised coating.EFFECT: technical result is improved formation of monoclonal clusters and purity of the main component of the cluster in wells with polyclonal clusters which additionally increases sequencing yield and data quality.19 cl, 8 dwg, 2 ex
Изобретение относится к биологическим матрицам, которые могут быть применены для проведения генетического секвенирования, а также для обнаружения и исследования разнообразных молекул, семейств молекул, уровней экспрессии генов, однонуклеотидных полиморфизмов и для проведения генотипирования. Описана проточная ячейка, содержащая структуированную подложку, включающую углубления. Слой функционализированного покрытия наносят на углубления структурированной подложки проточной ячейки. Углубления отделены друг от друга промежуточными участками. Праймер привит к слою функционализированного покрытия, в результате чего образуется привитой слой функционализированного покрытия, находящегося в углублениях. Гидрогель наносят на по меньшей мере привитой слой функционализированного покрытия. Технический результат - улучшение образования моноклональных кластеров и чистоты основного компонента кластера в лунках с поликлональными кластерами, что также повышает выход секвенирования и качество данных. 3 н. и 16 з.п. ф-лы, 8 ил., 2 пр.</description><subject>ADHESIVES</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY</subject><subject>CHEMICAL PAINT OR INK REMOVERS</subject><subject>CHEMISTRY</subject><subject>COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS</subject><subject>COMPOSITIONS BASED THEREON</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CORRECTING FLUIDS</subject><subject>DYES</subject><subject>ENZYMOLOGY</subject><subject>FILLING PASTES</subject><subject>INKS</subject><subject>MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MISCELLANEOUS APPLICATIONS OF MATERIALS</subject><subject>MISCELLANEOUS COMPOSITIONS</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NATURAL RESINS</subject><subject>ORGANIC MACROMOLECULAR COMPOUNDS</subject><subject>PAINTS</subject><subject>PASTES OR SOLIDS FOR COLOURING OR PRINTING</subject><subject>PERFORMING OPERATIONS</subject><subject>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</subject><subject>POLISHES</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>THEIR PREPARATION OR CHEMICAL WORKING-UP</subject><subject>THEIR RELEVANT APPARATUS</subject><subject>TRANSPORTING</subject><subject>USE OF MATERIALS THEREFOR</subject><subject>VINEGAR</subject><subject>WINE</subject><subject>WOODSTAINS</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNBw8_EP1w3xCPIPdfdQcHb18QlWCPcM8VDwiHQJ8nd39VFw9ncM8fRz52FgTUvMKU7lhdLcDApuriHOHrqpBfnxqcUFicmpeakl8UGhRuamhqaG5s5GxkQoAQDSICPx</recordid><startdate>20210714</startdate><enddate>20210714</enddate><creator>ZHEN, Khuntszi</creator><creator>BOUTELL, Dzhonatan Mark</creator><creator>BOUEN, M. Shejn</creator><creator>MUN, Dzhon A</creator><creator>SKINNER, Gari Mark</creator><creator>CHEN, Kenni</creator><creator>NEMIROSKI, Aleks</creator><scope>EVB</scope></search><sort><creationdate>20210714</creationdate><title>FLOW-THROUGH CELLS WITH HYDROGEL COATING</title><author>ZHEN, Khuntszi ; BOUTELL, Dzhonatan Mark ; BOUEN, M. Shejn ; MUN, Dzhon A ; SKINNER, Gari Mark ; CHEN, Kenni ; NEMIROSKI, Aleks</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_RU2751517C23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rus</language><creationdate>2021</creationdate><topic>ADHESIVES</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY</topic><topic>CHEMICAL PAINT OR INK REMOVERS</topic><topic>CHEMISTRY</topic><topic>COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS</topic><topic>COMPOSITIONS BASED THEREON</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CORRECTING FLUIDS</topic><topic>DYES</topic><topic>ENZYMOLOGY</topic><topic>FILLING PASTES</topic><topic>INKS</topic><topic>MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MISCELLANEOUS APPLICATIONS OF MATERIALS</topic><topic>MISCELLANEOUS COMPOSITIONS</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NATURAL RESINS</topic><topic>ORGANIC MACROMOLECULAR COMPOUNDS</topic><topic>PAINTS</topic><topic>PASTES OR SOLIDS FOR COLOURING OR PRINTING</topic><topic>PERFORMING OPERATIONS</topic><topic>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</topic><topic>POLISHES</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>THEIR PREPARATION OR CHEMICAL WORKING-UP</topic><topic>THEIR RELEVANT APPARATUS</topic><topic>TRANSPORTING</topic><topic>USE OF MATERIALS THEREFOR</topic><topic>VINEGAR</topic><topic>WINE</topic><topic>WOODSTAINS</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHEN, Khuntszi</creatorcontrib><creatorcontrib>BOUTELL, Dzhonatan Mark</creatorcontrib><creatorcontrib>BOUEN, M. Shejn</creatorcontrib><creatorcontrib>MUN, Dzhon A</creatorcontrib><creatorcontrib>SKINNER, Gari Mark</creatorcontrib><creatorcontrib>CHEN, Kenni</creatorcontrib><creatorcontrib>NEMIROSKI, Aleks</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHEN, Khuntszi</au><au>BOUTELL, Dzhonatan Mark</au><au>BOUEN, M. Shejn</au><au>MUN, Dzhon A</au><au>SKINNER, Gari Mark</au><au>CHEN, Kenni</au><au>NEMIROSKI, Aleks</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>FLOW-THROUGH CELLS WITH HYDROGEL COATING</title><date>2021-07-14</date><risdate>2021</risdate><abstract>FIELD: genetics.SUBSTANCE: invention relates to biological matrices applicable for genetic sequencing and for detection and research of various molecules, families of molecules, levels of gene expression, single nucleotide polymorphisms, and for genotyping. Described is a flow-through cell containing a structured substrate including recesses. A layer of functionalised coating is applied to the recesses of the structured substrate of the flow-through cell. The recesses are separated from each other by intermediate sections. The primer is grafted to the layer of the functionalised coating resulting in a grafted layer of the functionalised coating located in the recesses. The hydrogel is applied to at least the grafted layer of the functionalised coating.EFFECT: technical result is improved formation of monoclonal clusters and purity of the main component of the cluster in wells with polyclonal clusters which additionally increases sequencing yield and data quality.19 cl, 8 dwg, 2 ex
Изобретение относится к биологическим матрицам, которые могут быть применены для проведения генетического секвенирования, а также для обнаружения и исследования разнообразных молекул, семейств молекул, уровней экспрессии генов, однонуклеотидных полиморфизмов и для проведения генотипирования. Описана проточная ячейка, содержащая структуированную подложку, включающую углубления. Слой функционализированного покрытия наносят на углубления структурированной подложки проточной ячейки. Углубления отделены друг от друга промежуточными участками. Праймер привит к слою функционализированного покрытия, в результате чего образуется привитой слой функционализированного покрытия, находящегося в углублениях. Гидрогель наносят на по меньшей мере привитой слой функционализированного покрытия. Технический результат - улучшение образования моноклональных кластеров и чистоты основного компонента кластера в лунках с поликлональными кластерами, что также повышает выход секвенирования и качество данных. 3 н. и 16 з.п. ф-лы, 8 ил., 2 пр.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ADHESIVES BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMICAL PAINT OR INK REMOVERS CHEMISTRY COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS COMPOSITIONS BASED THEREON COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CORRECTING FLUIDS DYES ENZYMOLOGY FILLING PASTES INKS MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MUTATION OR GENETIC ENGINEERING NATURAL RESINS ORGANIC MACROMOLECULAR COMPOUNDS PAINTS PASTES OR SOLIDS FOR COLOURING OR PRINTING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL POLISHES PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP THEIR RELEVANT APPARATUS TRANSPORTING USE OF MATERIALS THEREFOR VINEGAR WINE WOODSTAINS |
| title | FLOW-THROUGH CELLS WITH HYDROGEL COATING |
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