METHOD FOR ELIMINATING FALSE-POSITIVE RESULTS CAUSED BY CONTAMINATION OF DNA POLYMERASE WITH FOREIGN DNA FRAGMENTS, WITH IDENTIFICATION OF BLATEM GENES
FIELD: medicine.SUBSTANCE: invention relates to molecular biology, microbiology and medicine. Invention includes a set of original oligonucleotide primers for method implementation, as well as an algorithm for selecting primers which are non-specific to the detected DNA fragments and capable of ampl...
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| creator | Mikhajlovich Vladimir Mikhajlovich Gejdarov Rustam Nuraddin ogly Masalov Yaroslav Konstantinovich |
| description | FIELD: medicine.SUBSTANCE: invention relates to molecular biology, microbiology and medicine. Invention includes a set of original oligonucleotide primers for method implementation, as well as an algorithm for selecting primers which are non-specific to the detected DNA fragments and capable of amplifying another fragment of the blaTEM gene in the analysed sample.EFFECT: invention provides a method for eliminating false-positive results caused by contamination of DNA polymerases when detecting blaTEM genes of resistance to β-lactam antibiotics.2 cl, 2 dwg, 1 tbl, 1 ex
Изобретение относится к молекулярной биологии, микробиологии и медицине. Изобретение включает набор оригинальных олигонуклеотидных праймеров для осуществления способа, а также алгоритм выбора праймеров, неспецифичных к обнаруженным фрагментам контаминирующей ДНК и способных амплифицировать иной фрагмент гена blaTEM в изучаемом образце. Изобретение обеспечивает способ устранения ложноположительных результатов, вызванных контаминацией ДНК-полимераз при детекции blaTEM-генов устойчивости к β-лактамным антибиотикам. 2 н.п. ф-лы, 2 ил., 1 табл., 1 пр. |
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Изобретение относится к молекулярной биологии, микробиологии и медицине. Изобретение включает набор оригинальных олигонуклеотидных праймеров для осуществления способа, а также алгоритм выбора праймеров, неспецифичных к обнаруженным фрагментам контаминирующей ДНК и способных амплифицировать иной фрагмент гена blaTEM в изучаемом образце. Изобретение обеспечивает способ устранения ложноположительных результатов, вызванных контаминацией ДНК-полимераз при детекции blaTEM-генов устойчивости к β-лактамным антибиотикам. 2 н.п. ф-лы, 2 ил., 1 табл., 1 пр.</description><language>eng ; rus</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200729&DB=EPODOC&CC=RU&NR=2728317C1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200729&DB=EPODOC&CC=RU&NR=2728317C1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Mikhajlovich Vladimir Mikhajlovich</creatorcontrib><creatorcontrib>Gejdarov Rustam Nuraddin ogly</creatorcontrib><creatorcontrib>Masalov Yaroslav Konstantinovich</creatorcontrib><title>METHOD FOR ELIMINATING FALSE-POSITIVE RESULTS CAUSED BY CONTAMINATION OF DNA POLYMERASE WITH FOREIGN DNA FRAGMENTS, WITH IDENTIFICATION OF BLATEM GENES</title><description>FIELD: medicine.SUBSTANCE: invention relates to molecular biology, microbiology and medicine. Invention includes a set of original oligonucleotide primers for method implementation, as well as an algorithm for selecting primers which are non-specific to the detected DNA fragments and capable of amplifying another fragment of the blaTEM gene in the analysed sample.EFFECT: invention provides a method for eliminating false-positive results caused by contamination of DNA polymerases when detecting blaTEM genes of resistance to β-lactam antibiotics.2 cl, 2 dwg, 1 tbl, 1 ex
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Изобретение относится к молекулярной биологии, микробиологии и медицине. Изобретение включает набор оригинальных олигонуклеотидных праймеров для осуществления способа, а также алгоритм выбора праймеров, неспецифичных к обнаруженным фрагментам контаминирующей ДНК и способных амплифицировать иной фрагмент гена blaTEM в изучаемом образце. Изобретение обеспечивает способ устранения ложноположительных результатов, вызванных контаминацией ДНК-полимераз при детекции blaTEM-генов устойчивости к β-лактамным антибиотикам. 2 н.п. ф-лы, 2 ил., 1 табл., 1 пр.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | METHOD FOR ELIMINATING FALSE-POSITIVE RESULTS CAUSED BY CONTAMINATION OF DNA POLYMERASE WITH FOREIGN DNA FRAGMENTS, WITH IDENTIFICATION OF BLATEM GENES |
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