RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN
FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology, in particular to genetic engineering and in particular to the recombinant protein L-HEP-HG6-CBD, recombinant protein, which is used to produce recombinant protein L-HEP-HG6-CBD, recombinant plasmid DNA pET32b-L-HEP-HG6-...
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| creator | Gasparyan Marine Eduardovna Kalinin Danil Sergeevich Korolev Ivan Romanovich Nokel Elena Adamovna Pavlenko Daniil Mikhajlovich Makarov Dmitrij Aleksandrovich Sokolova Irina Vladimirovna Miroshnikov Anatolij Ivanovich Myagkikh Igor Valentinovich Sveshnikova Elena Vasilevna Melikhova Tatyana Dmitrievna Zinchenko Aleksej Alekseevich Puchkov Ilya Aleksandrovich Novozhilov Nikolaj Mikhajlovich Levandovskaya Kristina Georgievna Vorobeva Tatyana Vladimirovna Dolgikh Dmitrij Aleksandrovich Stepanenko Vasilij Nikolaevich Kirpichnikov Mikhail Petrovich |
| description | FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology, in particular to genetic engineering and in particular to the recombinant protein L-HEP-HG6-CBD, recombinant protein, which is used to produce recombinant protein L-HEP-HG6-CBD, recombinant plasmid DNA pET32b-L-HEP-HG6-CBD for expression of recombinant protein, strain Escherichia coli BL 21(DE3)/L-HEP-HG6-CBD, which produces recombinant protein, as well as a method for production of recombinant protein L-HEP-HG6-CBD.EFFECT: technical result of this invention consists in producing cells-producer of recombinant protein, having catalytic activity of light chain human enteropeptidase and capable of immobilisation on metal-chelate and chitin carriers without loss of catalytic activity.16 cl, 4 ex, 3 dwg
Изобретение относится к области биотехнологии, в частности к генной инженерии и в частности к рекомбинантному белку L-HEP-HG6-CBD, рекомбинантному белку, который используется для получения рекомбинантного белка L-HEP-HG6-CBD, рекомбинантной плазмидной ДНК pET32b-L-HEP-HG6-CBD для экспрессии рекомбинантного белка, штамму Escherichia coli BL 21(DE3)/L-HEP-HG6-CBD, который продуцирует рекомбинантный белок, а также способу получения рекомбинантного белка L-HEP-HG6-CBD. Технический результат данного изобретения заключается в получении клеток штамма-продуцента рекомбинантного белка, обладающего каталитической активностью лёгкой цепи энтеропептидазы человека и способного к иммобилизации на металл-хелатных и хитиновых носителях без потери каталитической активности. 5 н. и 11 з.п. ф-лы, 4 пр., 3 ил. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_RU2716975C1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>RU2716975C1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_RU2716975C13</originalsourceid><addsrcrecordid>eNqNzTEOwjAMBdAuDAi4gw9Ah4IAMQbHJZZap0rSuapQmBBUKhfhxlAKAxuTh__-9zR5OEJbHliUBKgK5UvWoEUBWs1yhLz2bAUqZwOxQJEaqpZAHg05RsMDLBh8cIrfTNdIDmwOXr2Wvj0lGkoKxuohGtkw__N9tPNkcm4vfVx87iyBnAKaNHa3JvZde4rXeG9cvdpl2_1ug9n6D_IE3zBAXg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN</title><source>esp@cenet</source><creator>Gasparyan Marine Eduardovna ; Kalinin Danil Sergeevich ; Korolev Ivan Romanovich ; Nokel Elena Adamovna ; Pavlenko Daniil Mikhajlovich ; Makarov Dmitrij Aleksandrovich ; Sokolova Irina Vladimirovna ; Miroshnikov Anatolij Ivanovich ; Myagkikh Igor Valentinovich ; Sveshnikova Elena Vasilevna ; Melikhova Tatyana Dmitrievna ; Zinchenko Aleksej Alekseevich ; Puchkov Ilya Aleksandrovich ; Novozhilov Nikolaj Mikhajlovich ; Levandovskaya Kristina Georgievna ; Vorobeva Tatyana Vladimirovna ; Dolgikh Dmitrij Aleksandrovich ; Stepanenko Vasilij Nikolaevich ; Kirpichnikov Mikhail Petrovich</creator><creatorcontrib>Gasparyan Marine Eduardovna ; Kalinin Danil Sergeevich ; Korolev Ivan Romanovich ; Nokel Elena Adamovna ; Pavlenko Daniil Mikhajlovich ; Makarov Dmitrij Aleksandrovich ; Sokolova Irina Vladimirovna ; Miroshnikov Anatolij Ivanovich ; Myagkikh Igor Valentinovich ; Sveshnikova Elena Vasilevna ; Melikhova Tatyana Dmitrievna ; Zinchenko Aleksej Alekseevich ; Puchkov Ilya Aleksandrovich ; Novozhilov Nikolaj Mikhajlovich ; Levandovskaya Kristina Georgievna ; Vorobeva Tatyana Vladimirovna ; Dolgikh Dmitrij Aleksandrovich ; Stepanenko Vasilij Nikolaevich ; Kirpichnikov Mikhail Petrovich</creatorcontrib><description>FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology, in particular to genetic engineering and in particular to the recombinant protein L-HEP-HG6-CBD, recombinant protein, which is used to produce recombinant protein L-HEP-HG6-CBD, recombinant plasmid DNA pET32b-L-HEP-HG6-CBD for expression of recombinant protein, strain Escherichia coli BL 21(DE3)/L-HEP-HG6-CBD, which produces recombinant protein, as well as a method for production of recombinant protein L-HEP-HG6-CBD.EFFECT: technical result of this invention consists in producing cells-producer of recombinant protein, having catalytic activity of light chain human enteropeptidase and capable of immobilisation on metal-chelate and chitin carriers without loss of catalytic activity.16 cl, 4 ex, 3 dwg
Изобретение относится к области биотехнологии, в частности к генной инженерии и в частности к рекомбинантному белку L-HEP-HG6-CBD, рекомбинантному белку, который используется для получения рекомбинантного белка L-HEP-HG6-CBD, рекомбинантной плазмидной ДНК pET32b-L-HEP-HG6-CBD для экспрессии рекомбинантного белка, штамму Escherichia coli BL 21(DE3)/L-HEP-HG6-CBD, который продуцирует рекомбинантный белок, а также способу получения рекомбинантного белка L-HEP-HG6-CBD. Технический результат данного изобретения заключается в получении клеток штамма-продуцента рекомбинантного белка, обладающего каталитической активностью лёгкой цепи энтеропептидазы человека и способного к иммобилизации на металл-хелатных и хитиновых носителях без потери каталитической активности. 5 н. и 11 з.п. ф-лы, 4 пр., 3 ил.</description><language>eng ; rus</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200317&DB=EPODOC&CC=RU&NR=2716975C1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200317&DB=EPODOC&CC=RU&NR=2716975C1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Gasparyan Marine Eduardovna</creatorcontrib><creatorcontrib>Kalinin Danil Sergeevich</creatorcontrib><creatorcontrib>Korolev Ivan Romanovich</creatorcontrib><creatorcontrib>Nokel Elena Adamovna</creatorcontrib><creatorcontrib>Pavlenko Daniil Mikhajlovich</creatorcontrib><creatorcontrib>Makarov Dmitrij Aleksandrovich</creatorcontrib><creatorcontrib>Sokolova Irina Vladimirovna</creatorcontrib><creatorcontrib>Miroshnikov Anatolij Ivanovich</creatorcontrib><creatorcontrib>Myagkikh Igor Valentinovich</creatorcontrib><creatorcontrib>Sveshnikova Elena Vasilevna</creatorcontrib><creatorcontrib>Melikhova Tatyana Dmitrievna</creatorcontrib><creatorcontrib>Zinchenko Aleksej Alekseevich</creatorcontrib><creatorcontrib>Puchkov Ilya Aleksandrovich</creatorcontrib><creatorcontrib>Novozhilov Nikolaj Mikhajlovich</creatorcontrib><creatorcontrib>Levandovskaya Kristina Georgievna</creatorcontrib><creatorcontrib>Vorobeva Tatyana Vladimirovna</creatorcontrib><creatorcontrib>Dolgikh Dmitrij Aleksandrovich</creatorcontrib><creatorcontrib>Stepanenko Vasilij Nikolaevich</creatorcontrib><creatorcontrib>Kirpichnikov Mikhail Petrovich</creatorcontrib><title>RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN</title><description>FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology, in particular to genetic engineering and in particular to the recombinant protein L-HEP-HG6-CBD, recombinant protein, which is used to produce recombinant protein L-HEP-HG6-CBD, recombinant plasmid DNA pET32b-L-HEP-HG6-CBD for expression of recombinant protein, strain Escherichia coli BL 21(DE3)/L-HEP-HG6-CBD, which produces recombinant protein, as well as a method for production of recombinant protein L-HEP-HG6-CBD.EFFECT: technical result of this invention consists in producing cells-producer of recombinant protein, having catalytic activity of light chain human enteropeptidase and capable of immobilisation on metal-chelate and chitin carriers without loss of catalytic activity.16 cl, 4 ex, 3 dwg
Изобретение относится к области биотехнологии, в частности к генной инженерии и в частности к рекомбинантному белку L-HEP-HG6-CBD, рекомбинантному белку, который используется для получения рекомбинантного белка L-HEP-HG6-CBD, рекомбинантной плазмидной ДНК pET32b-L-HEP-HG6-CBD для экспрессии рекомбинантного белка, штамму Escherichia coli BL 21(DE3)/L-HEP-HG6-CBD, который продуцирует рекомбинантный белок, а также способу получения рекомбинантного белка L-HEP-HG6-CBD. Технический результат данного изобретения заключается в получении клеток штамма-продуцента рекомбинантного белка, обладающего каталитической активностью лёгкой цепи энтеропептидазы человека и способного к иммобилизации на металл-хелатных и хитиновых носителях без потери каталитической активности. 5 н. и 11 з.п. ф-лы, 4 пр., 3 ил.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNzTEOwjAMBdAuDAi4gw9Ah4IAMQbHJZZap0rSuapQmBBUKhfhxlAKAxuTh__-9zR5OEJbHliUBKgK5UvWoEUBWs1yhLz2bAUqZwOxQJEaqpZAHg05RsMDLBh8cIrfTNdIDmwOXr2Wvj0lGkoKxuohGtkw__N9tPNkcm4vfVx87iyBnAKaNHa3JvZde4rXeG9cvdpl2_1ug9n6D_IE3zBAXg</recordid><startdate>20200317</startdate><enddate>20200317</enddate><creator>Gasparyan Marine Eduardovna</creator><creator>Kalinin Danil Sergeevich</creator><creator>Korolev Ivan Romanovich</creator><creator>Nokel Elena Adamovna</creator><creator>Pavlenko Daniil Mikhajlovich</creator><creator>Makarov Dmitrij Aleksandrovich</creator><creator>Sokolova Irina Vladimirovna</creator><creator>Miroshnikov Anatolij Ivanovich</creator><creator>Myagkikh Igor Valentinovich</creator><creator>Sveshnikova Elena Vasilevna</creator><creator>Melikhova Tatyana Dmitrievna</creator><creator>Zinchenko Aleksej Alekseevich</creator><creator>Puchkov Ilya Aleksandrovich</creator><creator>Novozhilov Nikolaj Mikhajlovich</creator><creator>Levandovskaya Kristina Georgievna</creator><creator>Vorobeva Tatyana Vladimirovna</creator><creator>Dolgikh Dmitrij Aleksandrovich</creator><creator>Stepanenko Vasilij Nikolaevich</creator><creator>Kirpichnikov Mikhail Petrovich</creator><scope>EVB</scope></search><sort><creationdate>20200317</creationdate><title>RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN</title><author>Gasparyan Marine Eduardovna ; Kalinin Danil Sergeevich ; Korolev Ivan Romanovich ; Nokel Elena Adamovna ; Pavlenko Daniil Mikhajlovich ; Makarov Dmitrij Aleksandrovich ; Sokolova Irina Vladimirovna ; Miroshnikov Anatolij Ivanovich ; Myagkikh Igor Valentinovich ; Sveshnikova Elena Vasilevna ; Melikhova Tatyana Dmitrievna ; Zinchenko Aleksej Alekseevich ; Puchkov Ilya Aleksandrovich ; Novozhilov Nikolaj Mikhajlovich ; Levandovskaya Kristina Georgievna ; Vorobeva Tatyana Vladimirovna ; Dolgikh Dmitrij Aleksandrovich ; Stepanenko Vasilij Nikolaevich ; Kirpichnikov Mikhail Petrovich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_RU2716975C13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rus</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Gasparyan Marine Eduardovna</creatorcontrib><creatorcontrib>Kalinin Danil Sergeevich</creatorcontrib><creatorcontrib>Korolev Ivan Romanovich</creatorcontrib><creatorcontrib>Nokel Elena Adamovna</creatorcontrib><creatorcontrib>Pavlenko Daniil Mikhajlovich</creatorcontrib><creatorcontrib>Makarov Dmitrij Aleksandrovich</creatorcontrib><creatorcontrib>Sokolova Irina Vladimirovna</creatorcontrib><creatorcontrib>Miroshnikov Anatolij Ivanovich</creatorcontrib><creatorcontrib>Myagkikh Igor Valentinovich</creatorcontrib><creatorcontrib>Sveshnikova Elena Vasilevna</creatorcontrib><creatorcontrib>Melikhova Tatyana Dmitrievna</creatorcontrib><creatorcontrib>Zinchenko Aleksej Alekseevich</creatorcontrib><creatorcontrib>Puchkov Ilya Aleksandrovich</creatorcontrib><creatorcontrib>Novozhilov Nikolaj Mikhajlovich</creatorcontrib><creatorcontrib>Levandovskaya Kristina Georgievna</creatorcontrib><creatorcontrib>Vorobeva Tatyana Vladimirovna</creatorcontrib><creatorcontrib>Dolgikh Dmitrij Aleksandrovich</creatorcontrib><creatorcontrib>Stepanenko Vasilij Nikolaevich</creatorcontrib><creatorcontrib>Kirpichnikov Mikhail Petrovich</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Gasparyan Marine Eduardovna</au><au>Kalinin Danil Sergeevich</au><au>Korolev Ivan Romanovich</au><au>Nokel Elena Adamovna</au><au>Pavlenko Daniil Mikhajlovich</au><au>Makarov Dmitrij Aleksandrovich</au><au>Sokolova Irina Vladimirovna</au><au>Miroshnikov Anatolij Ivanovich</au><au>Myagkikh Igor Valentinovich</au><au>Sveshnikova Elena Vasilevna</au><au>Melikhova Tatyana Dmitrievna</au><au>Zinchenko Aleksej Alekseevich</au><au>Puchkov Ilya Aleksandrovich</au><au>Novozhilov Nikolaj Mikhajlovich</au><au>Levandovskaya Kristina Georgievna</au><au>Vorobeva Tatyana Vladimirovna</au><au>Dolgikh Dmitrij Aleksandrovich</au><au>Stepanenko Vasilij Nikolaevich</au><au>Kirpichnikov Mikhail Petrovich</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN</title><date>2020-03-17</date><risdate>2020</risdate><abstract>FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology, in particular to genetic engineering and in particular to the recombinant protein L-HEP-HG6-CBD, recombinant protein, which is used to produce recombinant protein L-HEP-HG6-CBD, recombinant plasmid DNA pET32b-L-HEP-HG6-CBD for expression of recombinant protein, strain Escherichia coli BL 21(DE3)/L-HEP-HG6-CBD, which produces recombinant protein, as well as a method for production of recombinant protein L-HEP-HG6-CBD.EFFECT: technical result of this invention consists in producing cells-producer of recombinant protein, having catalytic activity of light chain human enteropeptidase and capable of immobilisation on metal-chelate and chitin carriers without loss of catalytic activity.16 cl, 4 ex, 3 dwg
Изобретение относится к области биотехнологии, в частности к генной инженерии и в частности к рекомбинантному белку L-HEP-HG6-CBD, рекомбинантному белку, который используется для получения рекомбинантного белка L-HEP-HG6-CBD, рекомбинантной плазмидной ДНК pET32b-L-HEP-HG6-CBD для экспрессии рекомбинантного белка, штамму Escherichia coli BL 21(DE3)/L-HEP-HG6-CBD, который продуцирует рекомбинантный белок, а также способу получения рекомбинантного белка L-HEP-HG6-CBD. Технический результат данного изобретения заключается в получении клеток штамма-продуцента рекомбинантного белка, обладающего каталитической активностью лёгкой цепи энтеропептидазы человека и способного к иммобилизации на металл-хелатных и хитиновых носителях без потери каталитической активности. 5 н. и 11 з.п. ф-лы, 4 пр., 3 ил.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN |
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