METHOD FOR SPECIES DIFFERENTIATION OF PATHOGENIC BURCCHOLDERIAS USING MONOCLONAL ANTIBODIES
FIELD: biotechnologies.SUBSTANCE: invention relates to the biotechnology. Disclosed is a method for species differentiation of pathogenic burkholderia by solid-phase immunoassay. Method involves sensibilization of a plate with a mixture of antibodies specific simultaneously to the agents of glanders...
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| creator | Kuklina Galina Viktorovna Ipatov Sergej Sergeevich Gorshkov Anton Sergeevich Kytmanov Aleksej Aleksandrovich Pechenkin Denis Valerievich Eremkin Andrej Valentinovich Elagin Georgij Dmitrievich |
| description | FIELD: biotechnologies.SUBSTANCE: invention relates to the biotechnology. Disclosed is a method for species differentiation of pathogenic burkholderia by solid-phase immunoassay. Method involves sensibilization of a plate with a mixture of antibodies specific simultaneously to the agents of glanders and melioidosis, use of a pair of species-specific immunoperoxidase conjugates and paired introduction of the analyzed samples into the wells of the plate. Monoclonal antibodies are obtained by immunizing linear mice with cultures of glanders and melioidosis activators, fusion of immune splenocytes with murine myeloma cells, development of linear mice in the abdominal cavity and purification by liquid chromatography.EFFECT: method for species differentiation of pathogenic Burdcholderia using monoclonal antibodies is proposed.1 cl, 1 tbl, 1 ex
Изобретение относится к биотехнологии. Предложен способ видовой дифференциации патогенных буркхольдерий методом твердофазного иммуноферментного анализа. Способ включает сенсибилизацию планшета смесью антител, специфичных одновременно к возбудителям сапа и мелиоидоза, использование пары видоспецифичных иммунопероксидазных конъюгатов и парное внесение исследуемых проб в лунки планшета. Моноклональные антитела получают путем иммунизации линейных мышей культурами возбудителей сапа и мелиоидоза, слияния иммунных спленоцитов с клетками мышиной миеломы, наработки в брюшной полости линейных мышей и очистки методами жидкостной хроматографии. 1 табл., 1 пр. |
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Изобретение относится к биотехнологии. Предложен способ видовой дифференциации патогенных буркхольдерий методом твердофазного иммуноферментного анализа. Способ включает сенсибилизацию планшета смесью антител, специфичных одновременно к возбудителям сапа и мелиоидоза, использование пары видоспецифичных иммунопероксидазных конъюгатов и парное внесение исследуемых проб в лунки планшета. Моноклональные антитела получают путем иммунизации линейных мышей культурами возбудителей сапа и мелиоидоза, слияния иммунных спленоцитов с клетками мышиной миеломы, наработки в брюшной полости линейных мышей и очистки методами жидкостной хроматографии. 1 табл., 1 пр.</description><language>eng ; rus</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20191009&DB=EPODOC&CC=RU&NR=2702723C1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25569,76552</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20191009&DB=EPODOC&CC=RU&NR=2702723C1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Kuklina Galina Viktorovna</creatorcontrib><creatorcontrib>Ipatov Sergej Sergeevich</creatorcontrib><creatorcontrib>Gorshkov Anton Sergeevich</creatorcontrib><creatorcontrib>Kytmanov Aleksej Aleksandrovich</creatorcontrib><creatorcontrib>Pechenkin Denis Valerievich</creatorcontrib><creatorcontrib>Eremkin Andrej Valentinovich</creatorcontrib><creatorcontrib>Elagin Georgij Dmitrievich</creatorcontrib><title>METHOD FOR SPECIES DIFFERENTIATION OF PATHOGENIC BURCCHOLDERIAS USING MONOCLONAL ANTIBODIES</title><description>FIELD: biotechnologies.SUBSTANCE: invention relates to the biotechnology. Disclosed is a method for species differentiation of pathogenic burkholderia by solid-phase immunoassay. Method involves sensibilization of a plate with a mixture of antibodies specific simultaneously to the agents of glanders and melioidosis, use of a pair of species-specific immunoperoxidase conjugates and paired introduction of the analyzed samples into the wells of the plate. Monoclonal antibodies are obtained by immunizing linear mice with cultures of glanders and melioidosis activators, fusion of immune splenocytes with murine myeloma cells, development of linear mice in the abdominal cavity and purification by liquid chromatography.EFFECT: method for species differentiation of pathogenic Burdcholderia using monoclonal antibodies is proposed.1 cl, 1 tbl, 1 ex
Изобретение относится к биотехнологии. Предложен способ видовой дифференциации патогенных буркхольдерий методом твердофазного иммуноферментного анализа. Способ включает сенсибилизацию планшета смесью антител, специфичных одновременно к возбудителям сапа и мелиоидоза, использование пары видоспецифичных иммунопероксидазных конъюгатов и парное внесение исследуемых проб в лунки планшета. Моноклональные антитела получают путем иммунизации линейных мышей культурами возбудителей сапа и мелиоидоза, слияния иммунных спленоцитов с клетками мышиной миеломы, наработки в брюшной полости линейных мышей и очистки методами жидкостной хроматографии. 1 табл., 1 пр.</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjDEOwjAMALswIOAP_gASpEPn1HEaS21cJenEUFUoTAgqlf-LDDyA6Za721e3gZITA1YCxJGQKYJhaymQT6wTiwexMOpideQZoZ0CopPeUGAdYYrsOxjEC_bidQ-6dK2YMjpWu8fy3PLpx0MFlhK6c17fc97W5Z5f-TOHSTUX1agar_UfyhdDDzIK</recordid><startdate>20191009</startdate><enddate>20191009</enddate><creator>Kuklina Galina Viktorovna</creator><creator>Ipatov Sergej Sergeevich</creator><creator>Gorshkov Anton Sergeevich</creator><creator>Kytmanov Aleksej Aleksandrovich</creator><creator>Pechenkin Denis Valerievich</creator><creator>Eremkin Andrej Valentinovich</creator><creator>Elagin Georgij Dmitrievich</creator><scope>EVB</scope></search><sort><creationdate>20191009</creationdate><title>METHOD FOR SPECIES DIFFERENTIATION OF PATHOGENIC BURCCHOLDERIAS USING MONOCLONAL ANTIBODIES</title><author>Kuklina Galina Viktorovna ; Ipatov Sergej Sergeevich ; Gorshkov Anton Sergeevich ; Kytmanov Aleksej Aleksandrovich ; Pechenkin Denis Valerievich ; Eremkin Andrej Valentinovich ; Elagin Georgij Dmitrievich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_RU2702723C13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rus</language><creationdate>2019</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>Kuklina Galina Viktorovna</creatorcontrib><creatorcontrib>Ipatov Sergej Sergeevich</creatorcontrib><creatorcontrib>Gorshkov Anton Sergeevich</creatorcontrib><creatorcontrib>Kytmanov Aleksej Aleksandrovich</creatorcontrib><creatorcontrib>Pechenkin Denis Valerievich</creatorcontrib><creatorcontrib>Eremkin Andrej Valentinovich</creatorcontrib><creatorcontrib>Elagin Georgij Dmitrievich</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Kuklina Galina Viktorovna</au><au>Ipatov Sergej Sergeevich</au><au>Gorshkov Anton Sergeevich</au><au>Kytmanov Aleksej Aleksandrovich</au><au>Pechenkin Denis Valerievich</au><au>Eremkin Andrej Valentinovich</au><au>Elagin Georgij Dmitrievich</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR SPECIES DIFFERENTIATION OF PATHOGENIC BURCCHOLDERIAS USING MONOCLONAL ANTIBODIES</title><date>2019-10-09</date><risdate>2019</risdate><abstract>FIELD: biotechnologies.SUBSTANCE: invention relates to the biotechnology. Disclosed is a method for species differentiation of pathogenic burkholderia by solid-phase immunoassay. Method involves sensibilization of a plate with a mixture of antibodies specific simultaneously to the agents of glanders and melioidosis, use of a pair of species-specific immunoperoxidase conjugates and paired introduction of the analyzed samples into the wells of the plate. Monoclonal antibodies are obtained by immunizing linear mice with cultures of glanders and melioidosis activators, fusion of immune splenocytes with murine myeloma cells, development of linear mice in the abdominal cavity and purification by liquid chromatography.EFFECT: method for species differentiation of pathogenic Burdcholderia using monoclonal antibodies is proposed.1 cl, 1 tbl, 1 ex
Изобретение относится к биотехнологии. Предложен способ видовой дифференциации патогенных буркхольдерий методом твердофазного иммуноферментного анализа. Способ включает сенсибилизацию планшета смесью антител, специфичных одновременно к возбудителям сапа и мелиоидоза, использование пары видоспецифичных иммунопероксидазных конъюгатов и парное внесение исследуемых проб в лунки планшета. Моноклональные антитела получают путем иммунизации линейных мышей культурами возбудителей сапа и мелиоидоза, слияния иммунных спленоцитов с клетками мышиной миеломы, наработки в брюшной полости линейных мышей и очистки методами жидкостной хроматографии. 1 табл., 1 пр.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING |
| title | METHOD FOR SPECIES DIFFERENTIATION OF PATHOGENIC BURCCHOLDERIAS USING MONOCLONAL ANTIBODIES |
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