YEAST STRAIN PICHIA PASTORIS - PRODUCER OF XYLANASE

FIELD: biotechnology.SUBSTANCE: invention relates to microbiology and biotechnology. Disclosed is a recombinant yeast strain Pichia pastoris VKPM Y-4394 producing xylanase. Said strain has an inactivated AOX1 gene and contains a xyl gene which codes endo-1,4-β-xylanase from Paenibacillus brasilensis...

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Hauptverfasser: Kalinina Anna Nikolaevna, Sineokij Sergej Pavlovich, Gordeeva Tatyana Leonidovna, Borshchevskaya Larisa Nikolaevna, Agranovich Anneta Mikhajlovna
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creator Kalinina Anna Nikolaevna
Sineokij Sergej Pavlovich
Gordeeva Tatyana Leonidovna
Borshchevskaya Larisa Nikolaevna
Agranovich Anneta Mikhajlovna
description FIELD: biotechnology.SUBSTANCE: invention relates to microbiology and biotechnology. Disclosed is a recombinant yeast strain Pichia pastoris VKPM Y-4394 producing xylanase. Said strain has an inactivated AOX1 gene and contains a xyl gene which codes endo-1,4-β-xylanase from Paenibacillus brasilensis.EFFECT: strain produces xylanase in amount of 1728 units/ml of culture fluid.1 cl, 4 dwg, 2 ex Изобретение относится к микробиологии и биотехнологии. Предложен рекомбинантный штамм дрожжей Pichia pastoris ВКПМ Y-4394, продуцирующий ксиланазу. Указанный штамм имеет инактивированный ген АОХ1 и содержит ген xyl, кодирующий эндо-1,4-β-ксиланазу из Paenibacillus brasilensis. Штамм продуцирует ксиланазу в количестве 1728 ед/мл культуральной жидкости. 4 ил., 2 пр.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title YEAST STRAIN PICHIA PASTORIS - PRODUCER OF XYLANASE
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