TEST SYSTEM FOR DETECTION OF DNA OF LEPTOSPIROSIS CAUSATIVE AGENT (LEPTOSPIRA SPECIALES) IN AGRICULTURAL ANIMALS

FIELD: veterinary medicine.SUBSTANCE: invention relates to veterinary microbiology, in particular to the laboratory diagnosis of pathogens of infectious diseases, and in particular to means of diagnosing infection in animals. Test system for detecting the DNA of the causative agent of leptospirosis...

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Hauptverfasser: Vasilevich Fedor Ivanovich, Donnik Irina Mikhajlovna, Samujlenko Anatolij Yakovlevich, Drobin Yurij Dmitrievich, Koshchaev Andrej Georgievich, Lysenko Aleksandr Anatolievich, Kalashnikova Tatyana Valerevna, Grin Svetlana Anatolevna, Kulakova Mariya Aleksandrovna, Krivonos Roman Anatolevich, Bannov Vasilij Aleksandrovich, Shevkoplyas Vladimir Nikolaevich, Isaeva Albina Gennadievna, Kotelnikova Aleksandra Andreevna, Loretts Olga Gennadevna, Chernykh Oleg Yurevich, Malyshev Denis Vladislavovich
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creator Vasilevich Fedor Ivanovich
Donnik Irina Mikhajlovna
Samujlenko Anatolij Yakovlevich
Drobin Yurij Dmitrievich
Koshchaev Andrej Georgievich
Lysenko Aleksandr Anatolievich
Kalashnikova Tatyana Valerevna
Grin Svetlana Anatolevna
Kulakova Mariya Aleksandrovna
Krivonos Roman Anatolevich
Bannov Vasilij Aleksandrovich
Shevkoplyas Vladimir Nikolaevich
Isaeva Albina Gennadievna
Kotelnikova Aleksandra Andreevna
Loretts Olga Gennadevna
Chernykh Oleg Yurevich
Malyshev Denis Vladislavovich
description FIELD: veterinary medicine.SUBSTANCE: invention relates to veterinary microbiology, in particular to the laboratory diagnosis of pathogens of infectious diseases, and in particular to means of diagnosing infection in animals. Test system for detecting the DNA of the causative agent of leptospirosis (Leptospira spp.) in farm animals is described, including a buffer for carrying out the polymerase chain reaction, a mixture for its implementation consisting of deoxynucleoside triphosphates, primers and fluorescent probes specific for the causative agent of leptospirosis Leptospira spp. and for the internal control sample; mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE, internal control sample, negative control sample, positive control sample. For the internal control sample, bacteriophage T4 suspension with a concentration of 5×10copies of nucleotide sequences is used per 1 mcl. For a positive control sample, a mixture of recombinant plasmid DNA containing a fragment of the genome of the causative agent of leptospirosis (Leptospira spp. Lpts) and a fragment of the genome of bacteriophage T4, taken in a volume ratio of 1:1, is used.EFFECT: reduction of labor costs, time and consumables during the polymerase chain reaction.1 cl, 2 dwg, 4 tbl, 1 ex Изобретение относится к ветеринарной микробиологии, в частности к лабораторной диагностике возбудителей инфекционных заболеваний, а именно к средствам диагностики инфекции у животных. Описан тест-система для выявления ДНК возбудителя лептоспироза (Leptospira spp.) у сельскохозяйственных животных, включающий буфер для проведения полимеразной цепной реакции, смесь для ее проведения состоящая из дезоксинуклеозидтрифосфатов, праймеров и флуоресцентных зондов специфичные для возбудителя лептоспироза Leptospira spp. и для внутреннего контрольного образца; смесь ферментов из ДНК полимеразы с антителами, ингибирующих активность фермента, TAQ POLYMERASE, внутренний контрольный образец, отрицательный контрольный образец, положительный контрольный образец. Для внутреннего контрольного образца используют суспензию бактериофага Т4 с концентрацией 5×10копий нуклеотидных последовательностей на 1 мкл. Для положительного контрольного образца используют смесь рекомбинантных плазмидных ДНК, содержащих фрагмент генома возбудителя лептоспироза (Leptospira spp. Lpts) и фрагмент генома бактериофага Т4, взятых в объемном соотношении 1:1. Технический результат - уменьшение трудозатрат, времен
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Test system for detecting the DNA of the causative agent of leptospirosis (Leptospira spp.) in farm animals is described, including a buffer for carrying out the polymerase chain reaction, a mixture for its implementation consisting of deoxynucleoside triphosphates, primers and fluorescent probes specific for the causative agent of leptospirosis Leptospira spp. and for the internal control sample; mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE, internal control sample, negative control sample, positive control sample. For the internal control sample, bacteriophage T4 suspension with a concentration of 5×10copies of nucleotide sequences is used per 1 mcl. For a positive control sample, a mixture of recombinant plasmid DNA containing a fragment of the genome of the causative agent of leptospirosis (Leptospira spp. Lpts) and a fragment of the genome of bacteriophage T4, taken in a volume ratio of 1:1, is used.EFFECT: reduction of labor costs, time and consumables during the polymerase chain reaction.1 cl, 2 dwg, 4 tbl, 1 ex Изобретение относится к ветеринарной микробиологии, в частности к лабораторной диагностике возбудителей инфекционных заболеваний, а именно к средствам диагностики инфекции у животных. Описан тест-система для выявления ДНК возбудителя лептоспироза (Leptospira spp.) у сельскохозяйственных животных, включающий буфер для проведения полимеразной цепной реакции, смесь для ее проведения состоящая из дезоксинуклеозидтрифосфатов, праймеров и флуоресцентных зондов специфичные для возбудителя лептоспироза Leptospira spp. и для внутреннего контрольного образца; смесь ферментов из ДНК полимеразы с антителами, ингибирующих активность фермента, TAQ POLYMERASE, внутренний контрольный образец, отрицательный контрольный образец, положительный контрольный образец. Для внутреннего контрольного образца используют суспензию бактериофага Т4 с концентрацией 5×10копий нуклеотидных последовательностей на 1 мкл. Для положительного контрольного образца используют смесь рекомбинантных плазмидных ДНК, содержащих фрагмент генома возбудителя лептоспироза (Leptospira spp. Lpts) и фрагмент генома бактериофага Т4, взятых в объемном соотношении 1:1. Технический результат - уменьшение трудозатрат, времени и расходного материала при проведении полимеразной цепной реакции. 2 ил., 4 табл., 1 пр.</description><language>eng ; rus</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20190215&amp;DB=EPODOC&amp;CC=RU&amp;NR=2680094C1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25566,76549</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20190215&amp;DB=EPODOC&amp;CC=RU&amp;NR=2680094C1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Vasilevich Fedor Ivanovich</creatorcontrib><creatorcontrib>Donnik Irina Mikhajlovna</creatorcontrib><creatorcontrib>Samujlenko Anatolij Yakovlevich</creatorcontrib><creatorcontrib>Drobin Yurij Dmitrievich</creatorcontrib><creatorcontrib>Koshchaev Andrej Georgievich</creatorcontrib><creatorcontrib>Lysenko Aleksandr Anatolievich</creatorcontrib><creatorcontrib>Kalashnikova Tatyana Valerevna</creatorcontrib><creatorcontrib>Grin Svetlana Anatolevna</creatorcontrib><creatorcontrib>Kulakova Mariya Aleksandrovna</creatorcontrib><creatorcontrib>Krivonos Roman Anatolevich</creatorcontrib><creatorcontrib>Bannov Vasilij Aleksandrovich</creatorcontrib><creatorcontrib>Shevkoplyas Vladimir Nikolaevich</creatorcontrib><creatorcontrib>Isaeva Albina Gennadievna</creatorcontrib><creatorcontrib>Kotelnikova Aleksandra Andreevna</creatorcontrib><creatorcontrib>Loretts Olga Gennadevna</creatorcontrib><creatorcontrib>Chernykh Oleg Yurevich</creatorcontrib><creatorcontrib>Malyshev Denis Vladislavovich</creatorcontrib><title>TEST SYSTEM FOR DETECTION OF DNA OF LEPTOSPIROSIS CAUSATIVE AGENT (LEPTOSPIRA SPECIALES) IN AGRICULTURAL ANIMALS</title><description>FIELD: veterinary medicine.SUBSTANCE: invention relates to veterinary microbiology, in particular to the laboratory diagnosis of pathogens of infectious diseases, and in particular to means of diagnosing infection in animals. Test system for detecting the DNA of the causative agent of leptospirosis (Leptospira spp.) in farm animals is described, including a buffer for carrying out the polymerase chain reaction, a mixture for its implementation consisting of deoxynucleoside triphosphates, primers and fluorescent probes specific for the causative agent of leptospirosis Leptospira spp. and for the internal control sample; mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE, internal control sample, negative control sample, positive control sample. For the internal control sample, bacteriophage T4 suspension with a concentration of 5×10copies of nucleotide sequences is used per 1 mcl. For a positive control sample, a mixture of recombinant plasmid DNA containing a fragment of the genome of the causative agent of leptospirosis (Leptospira spp. Lpts) and a fragment of the genome of bacteriophage T4, taken in a volume ratio of 1:1, is used.EFFECT: reduction of labor costs, time and consumables during the polymerase chain reaction.1 cl, 2 dwg, 4 tbl, 1 ex Изобретение относится к ветеринарной микробиологии, в частности к лабораторной диагностике возбудителей инфекционных заболеваний, а именно к средствам диагностики инфекции у животных. Описан тест-система для выявления ДНК возбудителя лептоспироза (Leptospira spp.) у сельскохозяйственных животных, включающий буфер для проведения полимеразной цепной реакции, смесь для ее проведения состоящая из дезоксинуклеозидтрифосфатов, праймеров и флуоресцентных зондов специфичные для возбудителя лептоспироза Leptospira spp. и для внутреннего контрольного образца; смесь ферментов из ДНК полимеразы с антителами, ингибирующих активность фермента, TAQ POLYMERASE, внутренний контрольный образец, отрицательный контрольный образец, положительный контрольный образец. Для внутреннего контрольного образца используют суспензию бактериофага Т4 с концентрацией 5×10копий нуклеотидных последовательностей на 1 мкл. Для положительного контрольного образца используют смесь рекомбинантных плазмидных ДНК, содержащих фрагмент генома возбудителя лептоспироза (Leptospira spp. Lpts) и фрагмент генома бактериофага Т4, взятых в объемном соотношении 1:1. Технический результат - уменьшение трудозатрат, времени и расходного материала при проведении полимеразной цепной реакции. 2 ил., 4 табл., 1 пр.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyj0LwjAQgOEuDqL-hxt1EOoHRccjvepBmpTcRXAqReIkWqj_HxXE2ekdnnec9UqiIGdRqqHyAUpSMsrega-gdPiJpUa9NBy8sIDBKKh8IsADOYX5jxGkIcNoSRbA7u2BTbQaA1pAxzVamWaja3cb0uzbSQYVqTkuU_9o09B3l3RPzzbEdbHL8_3WrDZ_LC-AOze1</recordid><startdate>20190215</startdate><enddate>20190215</enddate><creator>Vasilevich Fedor Ivanovich</creator><creator>Donnik Irina Mikhajlovna</creator><creator>Samujlenko Anatolij Yakovlevich</creator><creator>Drobin Yurij Dmitrievich</creator><creator>Koshchaev Andrej Georgievich</creator><creator>Lysenko Aleksandr Anatolievich</creator><creator>Kalashnikova Tatyana Valerevna</creator><creator>Grin Svetlana Anatolevna</creator><creator>Kulakova Mariya Aleksandrovna</creator><creator>Krivonos Roman Anatolevich</creator><creator>Bannov Vasilij Aleksandrovich</creator><creator>Shevkoplyas Vladimir Nikolaevich</creator><creator>Isaeva Albina Gennadievna</creator><creator>Kotelnikova Aleksandra Andreevna</creator><creator>Loretts Olga Gennadevna</creator><creator>Chernykh Oleg Yurevich</creator><creator>Malyshev Denis Vladislavovich</creator><scope>EVB</scope></search><sort><creationdate>20190215</creationdate><title>TEST SYSTEM FOR DETECTION OF DNA OF LEPTOSPIROSIS CAUSATIVE AGENT (LEPTOSPIRA SPECIALES) IN AGRICULTURAL ANIMALS</title><author>Vasilevich Fedor Ivanovich ; Donnik Irina Mikhajlovna ; Samujlenko Anatolij Yakovlevich ; Drobin Yurij Dmitrievich ; Koshchaev Andrej Georgievich ; Lysenko Aleksandr Anatolievich ; Kalashnikova Tatyana Valerevna ; Grin Svetlana Anatolevna ; Kulakova Mariya Aleksandrovna ; Krivonos Roman Anatolevich ; Bannov Vasilij Aleksandrovich ; Shevkoplyas Vladimir Nikolaevich ; Isaeva Albina Gennadievna ; Kotelnikova Aleksandra Andreevna ; Loretts Olga Gennadevna ; Chernykh Oleg Yurevich ; Malyshev Denis Vladislavovich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_RU2680094C13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rus</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Vasilevich Fedor Ivanovich</creatorcontrib><creatorcontrib>Donnik Irina Mikhajlovna</creatorcontrib><creatorcontrib>Samujlenko Anatolij Yakovlevich</creatorcontrib><creatorcontrib>Drobin Yurij Dmitrievich</creatorcontrib><creatorcontrib>Koshchaev Andrej Georgievich</creatorcontrib><creatorcontrib>Lysenko Aleksandr Anatolievich</creatorcontrib><creatorcontrib>Kalashnikova Tatyana Valerevna</creatorcontrib><creatorcontrib>Grin Svetlana Anatolevna</creatorcontrib><creatorcontrib>Kulakova Mariya Aleksandrovna</creatorcontrib><creatorcontrib>Krivonos Roman Anatolevich</creatorcontrib><creatorcontrib>Bannov Vasilij Aleksandrovich</creatorcontrib><creatorcontrib>Shevkoplyas Vladimir Nikolaevich</creatorcontrib><creatorcontrib>Isaeva Albina Gennadievna</creatorcontrib><creatorcontrib>Kotelnikova Aleksandra Andreevna</creatorcontrib><creatorcontrib>Loretts Olga Gennadevna</creatorcontrib><creatorcontrib>Chernykh Oleg Yurevich</creatorcontrib><creatorcontrib>Malyshev Denis Vladislavovich</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Vasilevich Fedor Ivanovich</au><au>Donnik Irina Mikhajlovna</au><au>Samujlenko Anatolij Yakovlevich</au><au>Drobin Yurij Dmitrievich</au><au>Koshchaev Andrej Georgievich</au><au>Lysenko Aleksandr Anatolievich</au><au>Kalashnikova Tatyana Valerevna</au><au>Grin Svetlana Anatolevna</au><au>Kulakova Mariya Aleksandrovna</au><au>Krivonos Roman Anatolevich</au><au>Bannov Vasilij Aleksandrovich</au><au>Shevkoplyas Vladimir Nikolaevich</au><au>Isaeva Albina Gennadievna</au><au>Kotelnikova Aleksandra Andreevna</au><au>Loretts Olga Gennadevna</au><au>Chernykh Oleg Yurevich</au><au>Malyshev Denis Vladislavovich</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>TEST SYSTEM FOR DETECTION OF DNA OF LEPTOSPIROSIS CAUSATIVE AGENT (LEPTOSPIRA SPECIALES) IN AGRICULTURAL ANIMALS</title><date>2019-02-15</date><risdate>2019</risdate><abstract>FIELD: veterinary medicine.SUBSTANCE: invention relates to veterinary microbiology, in particular to the laboratory diagnosis of pathogens of infectious diseases, and in particular to means of diagnosing infection in animals. Test system for detecting the DNA of the causative agent of leptospirosis (Leptospira spp.) in farm animals is described, including a buffer for carrying out the polymerase chain reaction, a mixture for its implementation consisting of deoxynucleoside triphosphates, primers and fluorescent probes specific for the causative agent of leptospirosis Leptospira spp. and for the internal control sample; mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE, internal control sample, negative control sample, positive control sample. For the internal control sample, bacteriophage T4 suspension with a concentration of 5×10copies of nucleotide sequences is used per 1 mcl. For a positive control sample, a mixture of recombinant plasmid DNA containing a fragment of the genome of the causative agent of leptospirosis (Leptospira spp. Lpts) and a fragment of the genome of bacteriophage T4, taken in a volume ratio of 1:1, is used.EFFECT: reduction of labor costs, time and consumables during the polymerase chain reaction.1 cl, 2 dwg, 4 tbl, 1 ex Изобретение относится к ветеринарной микробиологии, в частности к лабораторной диагностике возбудителей инфекционных заболеваний, а именно к средствам диагностики инфекции у животных. Описан тест-система для выявления ДНК возбудителя лептоспироза (Leptospira spp.) у сельскохозяйственных животных, включающий буфер для проведения полимеразной цепной реакции, смесь для ее проведения состоящая из дезоксинуклеозидтрифосфатов, праймеров и флуоресцентных зондов специфичные для возбудителя лептоспироза Leptospira spp. и для внутреннего контрольного образца; смесь ферментов из ДНК полимеразы с антителами, ингибирующих активность фермента, TAQ POLYMERASE, внутренний контрольный образец, отрицательный контрольный образец, положительный контрольный образец. Для внутреннего контрольного образца используют суспензию бактериофага Т4 с концентрацией 5×10копий нуклеотидных последовательностей на 1 мкл. Для положительного контрольного образца используют смесь рекомбинантных плазмидных ДНК, содержащих фрагмент генома возбудителя лептоспироза (Leptospira spp. Lpts) и фрагмент генома бактериофага Т4, взятых в объемном соотношении 1:1. Технический результат - уменьшение трудозатрат, времени и расходного материала при проведении полимеразной цепной реакции. 2 ил., 4 табл., 1 пр.</abstract><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
title TEST SYSTEM FOR DETECTION OF DNA OF LEPTOSPIROSIS CAUSATIVE AGENT (LEPTOSPIRA SPECIALES) IN AGRICULTURAL ANIMALS
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