METHOD FOR INHIBITING NUCLEAR FACTOR KAPPA B WITH USE OF 5-HYDROXYNICOTINATE 3-(2,2,2-TRIMETHYLHYDRAZINIUM) POTASSIUM PROPIONATE IN CELL CULTURE
FIELD: medicine.SUBSTANCE: invention relates to medicine, in particular to experimental pharmacology, and can be used to inhibit the nuclear factor kappa B (NF-kB). This method involves the addition of bacterial lipopolysaccharide at a concentration of 1 mcg/ml to the mononuclear blood cells of Wist...
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| creator | Peresypkina Anna Aleksandrovna Khavanskij Anatolij Vyacheslavovich Beskhmelnitsina Evgeniya Aleksandrovna Kochkarova Indira Sultanovna Kostina Darya Aleksandrovna Pokrovskij Mikhail Vladimirovich Kesarev Oleg Georgievich Skachilova Sofiya Yakovlevna Burda Yurij Evgenevich Danilenko Lyudmila Mikhajlovna Pasenov Konstantin Nikolaevich |
| description | FIELD: medicine.SUBSTANCE: invention relates to medicine, in particular to experimental pharmacology, and can be used to inhibit the nuclear factor kappa B (NF-kB). This method involves the addition of bacterial lipopolysaccharide at a concentration of 1 mcg/ml to the mononuclear blood cells of Wistar rats freshly isolated by the standard method of velocity sedimentation on gradient of Ficoll, which is followed by adding 5-hydroxynicotinate 3-(2,2,2-trimethylhydrazinium) potassium propionate, dissolved in phosphate-buffered saline, at a final concentration of 35 mcg/ml.EFFECT: method allows to evaluate the inhibitory NF-kB activity of 5-hydroxynicotinate 3-(2,2,2-trimethylhydrazinium) potassium propionate.1 cl, 1 tbl, 1 ex
Изобретение относится к медицине, в частности к экспериментальной фармакологии, и может быть использовано для ингибирования нуклеарного фактора каппа В (NF-kB). Способ включает добавление бактериального липополисахарида в концентрации 1 мкг/мл к свежевыделенным по стандартной методике на градиенте плотности фиколла мононуклеарным клеткам крови крыс Wistar, затем добавление к данной смеси 5-гидрокисиникотинат 3-(2,2,2-триметилгидразиний) пропионата калия, растворенного в фосфатно-солевом буфере, в конечной концентрации 35 мкг/мл. Способ позволяет оценить ингибирующую NF-kB активность 5-гидрокисиникотинат 3-(2,2,2-триметилгидразиний) пропионата калия. 1 табл.,1 пр. |
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Изобретение относится к медицине, в частности к экспериментальной фармакологии, и может быть использовано для ингибирования нуклеарного фактора каппа В (NF-kB). Способ включает добавление бактериального липополисахарида в концентрации 1 мкг/мл к свежевыделенным по стандартной методике на градиенте плотности фиколла мононуклеарным клеткам крови крыс Wistar, затем добавление к данной смеси 5-гидрокисиникотинат 3-(2,2,2-триметилгидразиний) пропионата калия, растворенного в фосфатно-солевом буфере, в конечной концентрации 35 мкг/мл. Способ позволяет оценить ингибирующую NF-kB активность 5-гидрокисиникотинат 3-(2,2,2-триметилгидразиний) пропионата калия. 1 табл.,1 пр.</description><language>eng ; rus</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20181210&DB=EPODOC&CC=RU&NR=2674443C1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25568,76551</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20181210&DB=EPODOC&CC=RU&NR=2674443C1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Peresypkina Anna Aleksandrovna</creatorcontrib><creatorcontrib>Khavanskij Anatolij Vyacheslavovich</creatorcontrib><creatorcontrib>Beskhmelnitsina Evgeniya Aleksandrovna</creatorcontrib><creatorcontrib>Kochkarova Indira Sultanovna</creatorcontrib><creatorcontrib>Kostina Darya Aleksandrovna</creatorcontrib><creatorcontrib>Pokrovskij Mikhail Vladimirovich</creatorcontrib><creatorcontrib>Kesarev Oleg Georgievich</creatorcontrib><creatorcontrib>Skachilova Sofiya Yakovlevna</creatorcontrib><creatorcontrib>Burda Yurij Evgenevich</creatorcontrib><creatorcontrib>Danilenko Lyudmila Mikhajlovna</creatorcontrib><creatorcontrib>Pasenov Konstantin Nikolaevich</creatorcontrib><title>METHOD FOR INHIBITING NUCLEAR FACTOR KAPPA B WITH USE OF 5-HYDROXYNICOTINATE 3-(2,2,2-TRIMETHYLHYDRAZINIUM) POTASSIUM PROPIONATE IN CELL CULTURE</title><description>FIELD: medicine.SUBSTANCE: invention relates to medicine, in particular to experimental pharmacology, and can be used to inhibit the nuclear factor kappa B (NF-kB). This method involves the addition of bacterial lipopolysaccharide at a concentration of 1 mcg/ml to the mononuclear blood cells of Wistar rats freshly isolated by the standard method of velocity sedimentation on gradient of Ficoll, which is followed by adding 5-hydroxynicotinate 3-(2,2,2-trimethylhydrazinium) potassium propionate, dissolved in phosphate-buffered saline, at a final concentration of 35 mcg/ml.EFFECT: method allows to evaluate the inhibitory NF-kB activity of 5-hydroxynicotinate 3-(2,2,2-trimethylhydrazinium) potassium propionate.1 cl, 1 tbl, 1 ex
Изобретение относится к медицине, в частности к экспериментальной фармакологии, и может быть использовано для ингибирования нуклеарного фактора каппа В (NF-kB). Способ включает добавление бактериального липополисахарида в концентрации 1 мкг/мл к свежевыделенным по стандартной методике на градиенте плотности фиколла мононуклеарным клеткам крови крыс Wistar, затем добавление к данной смеси 5-гидрокисиникотинат 3-(2,2,2-триметилгидразиний) пропионата калия, растворенного в фосфатно-солевом буфере, в конечной концентрации 35 мкг/мл. 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This method involves the addition of bacterial lipopolysaccharide at a concentration of 1 mcg/ml to the mononuclear blood cells of Wistar rats freshly isolated by the standard method of velocity sedimentation on gradient of Ficoll, which is followed by adding 5-hydroxynicotinate 3-(2,2,2-trimethylhydrazinium) potassium propionate, dissolved in phosphate-buffered saline, at a final concentration of 35 mcg/ml.EFFECT: method allows to evaluate the inhibitory NF-kB activity of 5-hydroxynicotinate 3-(2,2,2-trimethylhydrazinium) potassium propionate.1 cl, 1 tbl, 1 ex
Изобретение относится к медицине, в частности к экспериментальной фармакологии, и может быть использовано для ингибирования нуклеарного фактора каппа В (NF-kB). Способ включает добавление бактериального липополисахарида в концентрации 1 мкг/мл к свежевыделенным по стандартной методике на градиенте плотности фиколла мононуклеарным клеткам крови крыс Wistar, затем добавление к данной смеси 5-гидрокисиникотинат 3-(2,2,2-триметилгидразиний) пропионата калия, растворенного в фосфатно-солевом буфере, в конечной концентрации 35 мкг/мл. Способ позволяет оценить ингибирующую NF-kB активность 5-гидрокисиникотинат 3-(2,2,2-триметилгидразиний) пропионата калия. 1 табл.,1 пр.</abstract><oa>free_for_read</oa></addata></record> |
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| title | METHOD FOR INHIBITING NUCLEAR FACTOR KAPPA B WITH USE OF 5-HYDROXYNICOTINATE 3-(2,2,2-TRIMETHYLHYDRAZINIUM) POTASSIUM PROPIONATE IN CELL CULTURE |
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