RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I FOR PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I, Pichia pastoris YEAST STRAIN - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I AND METHOD OF PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I
FIELD: genetic engineering.SUBSTANCE: to increase yield of mature human apolipoprotein A-I it is produced by enzymatic hydrolysis of SUMO-specific protease SP2 of recombinant chimeric protein SUMO3-apoA-I, synthesised by Pichia pastoris yeast strain. Said strain is constructed by transformation of P...
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| creator | Beklemishev Anatolij Borisovich Mamaev Aleksej Lvovich |
| description | FIELD: genetic engineering.SUBSTANCE: to increase yield of mature human apolipoprotein A-I it is produced by enzymatic hydrolysis of SUMO-specific protease SP2 of recombinant chimeric protein SUMO3-apoA-I, synthesised by Pichia pastoris yeast strain. Said strain is constructed by transformation of Pichia pastoris X-33 yeast strain with linearised plasmid DNA pPsecSUMO3-apoA-I. Recombinant chimeric protein SUMO3-apoA-I includes from N-terminal amino acid sequence of ubiquitin-like protein of human SUMO3, fused at C-terminal with amino acid sequence of mature human apoA-I protein.EFFECT: invention can be used for producing mature human apolipoprotein A-I.3 cl, 1 tbl, 5 dwg, 7 ex
Изобретение относится к генной инженерии и может быть использовано для получения зрелого аполипопротеина A-I человека. Для увеличения выхода зрелого аполипопротеина A-I человека его получают путем ферментативного гидролиза SUMO-специфичной протеазой SP2 рекомбинантного химерного белка SUMO3-апоА-I, синтезированного штаммом дрожжей Pichia pastoris. Указанный штамм сконструирован путем трансформации штамма дрожжей Pichia pastoris Х-33 линеаризованной плазмидной ДНК pPsecSUMO3-apoA-I. Рекомбинантный химерный белок SUMO3-apoA-I включает с N-конца аминокислотную последовательность убиквитин-подобного белка SUMO3 человека, слитую с С-конца с аминокислотной последовательностью зрелого белка апоА-I человека. 3 н.п. ф-лы, 1 табл., 5 ил., 7 пр. |
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Изобретение относится к генной инженерии и может быть использовано для получения зрелого аполипопротеина A-I человека. Для увеличения выхода зрелого аполипопротеина A-I человека его получают путем ферментативного гидролиза SUMO-специфичной протеазой SP2 рекомбинантного химерного белка SUMO3-апоА-I, синтезированного штаммом дрожжей Pichia pastoris. Указанный штамм сконструирован путем трансформации штамма дрожжей Pichia pastoris Х-33 линеаризованной плазмидной ДНК pPsecSUMO3-apoA-I. Рекомбинантный химерный белок SUMO3-apoA-I включает с N-конца аминокислотную последовательность убиквитин-подобного белка SUMO3 человека, слитую с С-конца с аминокислотной последовательностью зрелого белка апоА-I человека. 3 н.п. ф-лы, 1 табл., 5 ил., 7 пр.</description><language>eng ; rus</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161220&DB=EPODOC&CC=RU&NR=2605326C1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161220&DB=EPODOC&CC=RU&NR=2605326C1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Beklemishev Anatolij Borisovich</creatorcontrib><creatorcontrib>Mamaev Aleksej Lvovich</creatorcontrib><title>RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I FOR PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I, Pichia pastoris YEAST STRAIN - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I AND METHOD OF PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I</title><description>FIELD: genetic engineering.SUBSTANCE: to increase yield of mature human apolipoprotein A-I it is produced by enzymatic hydrolysis of SUMO-specific protease SP2 of recombinant chimeric protein SUMO3-apoA-I, synthesised by Pichia pastoris yeast strain. Said strain is constructed by transformation of Pichia pastoris X-33 yeast strain with linearised plasmid DNA pPsecSUMO3-apoA-I. Recombinant chimeric protein SUMO3-apoA-I includes from N-terminal amino acid sequence of ubiquitin-like protein of human SUMO3, fused at C-terminal with amino acid sequence of mature human apoA-I protein.EFFECT: invention can be used for producing mature human apolipoprotein A-I.3 cl, 1 tbl, 5 dwg, 7 ex
Изобретение относится к генной инженерии и может быть использовано для получения зрелого аполипопротеина A-I человека. Для увеличения выхода зрелого аполипопротеина A-I человека его получают путем ферментативного гидролиза SUMO-специфичной протеазой SP2 рекомбинантного химерного белка SUMO3-апоА-I, синтезированного штаммом дрожжей Pichia pastoris. Указанный штамм сконструирован путем трансформации штамма дрожжей Pichia pastoris Х-33 линеаризованной плазмидной ДНК pPsecSUMO3-apoA-I. Рекомбинантный химерный белок SUMO3-apoA-I включает с N-конца аминокислотную последовательность убиквитин-подобного белка SUMO3 человека, слитую с С-конца с аминокислотной последовательностью зрелого белка апоА-I человека. 3 н.п. ф-лы, 1 табл., 5 ил., 7 пр.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2016</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjk0KwjAYRLtxIeodvgMY0BbdxzQ1AfNDmixclVAiFsQG0yt7D1uo-65mMe8Ns86-hhIlLlxiaYEwLqjhBLRRlnIJtROqQD72GHGolJmK0hEuryCwdYYCcwJLwFrduFZ_baT3oLv22XmIPg39p0twp7i2UFuDRwLNS9SAqmD5ByxLENQyVU7e4jfbbPXwrxR2c24yqKglDIXYNyFF34Z3GBrj8vPhVORnciwWID_Rnlmo</recordid><startdate>20161220</startdate><enddate>20161220</enddate><creator>Beklemishev Anatolij Borisovich</creator><creator>Mamaev Aleksej Lvovich</creator><scope>EVB</scope></search><sort><creationdate>20161220</creationdate><title>RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I FOR PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I, Pichia pastoris YEAST STRAIN - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I AND METHOD OF PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I</title><author>Beklemishev Anatolij Borisovich ; Mamaev Aleksej Lvovich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_RU2605326C13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rus</language><creationdate>2016</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Beklemishev Anatolij Borisovich</creatorcontrib><creatorcontrib>Mamaev Aleksej Lvovich</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Beklemishev Anatolij Borisovich</au><au>Mamaev Aleksej Lvovich</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I FOR PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I, Pichia pastoris YEAST STRAIN - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I AND METHOD OF PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I</title><date>2016-12-20</date><risdate>2016</risdate><abstract>FIELD: genetic engineering.SUBSTANCE: to increase yield of mature human apolipoprotein A-I it is produced by enzymatic hydrolysis of SUMO-specific protease SP2 of recombinant chimeric protein SUMO3-apoA-I, synthesised by Pichia pastoris yeast strain. Said strain is constructed by transformation of Pichia pastoris X-33 yeast strain with linearised plasmid DNA pPsecSUMO3-apoA-I. Recombinant chimeric protein SUMO3-apoA-I includes from N-terminal amino acid sequence of ubiquitin-like protein of human SUMO3, fused at C-terminal with amino acid sequence of mature human apoA-I protein.EFFECT: invention can be used for producing mature human apolipoprotein A-I.3 cl, 1 tbl, 5 dwg, 7 ex
Изобретение относится к генной инженерии и может быть использовано для получения зрелого аполипопротеина A-I человека. Для увеличения выхода зрелого аполипопротеина A-I человека его получают путем ферментативного гидролиза SUMO-специфичной протеазой SP2 рекомбинантного химерного белка SUMO3-апоА-I, синтезированного штаммом дрожжей Pichia pastoris. Указанный штамм сконструирован путем трансформации штамма дрожжей Pichia pastoris Х-33 линеаризованной плазмидной ДНК pPsecSUMO3-apoA-I. Рекомбинантный химерный белок SUMO3-apoA-I включает с N-конца аминокислотную последовательность убиквитин-подобного белка SUMO3 человека, слитую с С-конца с аминокислотной последовательностью зрелого белка апоА-I человека. 3 н.п. ф-лы, 1 табл., 5 ил., 7 пр.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I FOR PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I, Pichia pastoris YEAST STRAIN - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I AND METHOD OF PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I |
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