METHOD OF DETERMINING DEGREE OF HETEROPLASMY MUTATIONS OF MITOCHONDRIAL GENOME

FIELD: medicine.SUBSTANCE: invention refers to medicine and aims at determining the degree of heteroplasmy of mitochondrial genome mutations. Polymerase chain reaction is carried out in real time, calculating the value of ΔCt, determined the coefficient of efficiency of amplification and a degree of...

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Hauptverfasser:	Sobenin Igor Aleksandrovich, Postnov Anton YUvenalevich, Mitrofanov Konstantin YUrevich
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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creator	Sobenin Igor Aleksandrovich Postnov Anton YUvenalevich Mitrofanov Konstantin YUrevich
description	FIELD: medicine.SUBSTANCE: invention refers to medicine and aims at determining the degree of heteroplasmy of mitochondrial genome mutations. Polymerase chain reaction is carried out in real time, calculating the value of ΔCt, determined the coefficient of efficiency of amplification and a degree of heteroplasmy of mutations of mitochondrial genome by formula.EFFECT: invention provides a simple and accessible for use in clinical practice method of determining heteroplasmy mutations of mitochondrial genome.1 cl, 5 ex Изобретение относится к области медицины и предназначено для определения степени гетероплазмии мутаций митохондриального генома. Проводят полимеразную цепную реакцию в режиме реального времени, вычисляют значение ΔCt, определяют коэффициент эффективности амплификации и рассчитывают степень гетероплазмии мутаций митохондриального генома по формуле. Изобретение обеспечивает простой и доступный для использования в клинической практике способ определения гетероплазмии мутаций митохондриального генома. 5 пр.
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Polymerase chain reaction is carried out in real time, calculating the value of ΔCt, determined the coefficient of efficiency of amplification and a degree of heteroplasmy of mutations of mitochondrial genome by formula.EFFECT: invention provides a simple and accessible for use in clinical practice method of determining heteroplasmy mutations of mitochondrial genome.1 cl, 5 ex Изобретение относится к области медицины и предназначено для определения степени гетероплазмии мутаций митохондриального генома. Проводят полимеразную цепную реакцию в режиме реального времени, вычисляют значение ΔCt, определяют коэффициент эффективности амплификации и рассчитывают степень гетероплазмии мутаций митохондриального генома по формуле. 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Polymerase chain reaction is carried out in real time, calculating the value of ΔCt, determined the coefficient of efficiency of amplification and a degree of heteroplasmy of mutations of mitochondrial genome by formula.EFFECT: invention provides a simple and accessible for use in clinical practice method of determining heteroplasmy mutations of mitochondrial genome.1 cl, 5 ex Изобретение относится к области медицины и предназначено для определения степени гетероплазмии мутаций митохондриального генома. Проводят полимеразную цепную реакцию в режиме реального времени, вычисляют значение ΔCt, определяют коэффициент эффективности амплификации и рассчитывают степень гетероплазмии мутаций митохондриального генома по формуле. 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subjects	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
title	METHOD OF DETERMINING DEGREE OF HETEROPLASMY MUTATIONS OF MITOCHONDRIAL GENOME
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