DEVICE FOR IDENTIFICATION OF NUCLEOTIDE SEQUENCES

FIELD: chemistry.SUBSTANCE: invention relates to the field of molecular biology and biochemistry. A device consists of a light source, the radiation of which is directed on a transparent substrate with oligonucleotides immobilised on its surface and a system of detecting the intensity of light, whic...

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Hauptverfasser: EROFEEV ALEKSANDR SERGEEVICH, GASKAROV ALMAZ AJRATOVICH, EGOROV ALEKSEJ MIKHAJLOVICH, JAMINSKIJ IGOR' VLADIMIROVICH, RUBTSOVA MAJJA JUR'EVNA, ULJASHOVA MARIJA MORISOVNA, IVANIN ANDREJ IGOREVICH, DUBROVIN EVGENIJ VLADIMIROVICH, PRESNOVA GALINA VASIL'EVNA, GRIGORENKO VITALIJ GEORGIEVICH
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creator EROFEEV ALEKSANDR SERGEEVICH
GASKAROV ALMAZ AJRATOVICH
EGOROV ALEKSEJ MIKHAJLOVICH
JAMINSKIJ IGOR' VLADIMIROVICH
RUBTSOVA MAJJA JUR'EVNA
ULJASHOVA MARIJA MORISOVNA
IVANIN ANDREJ IGOREVICH
DUBROVIN EVGENIJ VLADIMIROVICH
PRESNOVA GALINA VASIL'EVNA
GRIGORENKO VITALIJ GEORGIEVICH
description FIELD: chemistry.SUBSTANCE: invention relates to the field of molecular biology and biochemistry. A device consists of a light source, the radiation of which is directed on a transparent substrate with oligonucleotides immobilised on its surface and a system of detecting the intensity of light, which passed through the substrate, located under it. The substrate contains at least two zones, with a layer of oligonucleotides, non-specific to the nucleotide sequence under study, being immobilised on the surface of one of them, and a layer of nucleotides, specific to the nucleotide sequence under study, being immobilised on the surface of the other zone. The detection system contains at least two photosensitive independent sections, each of which is illuminated by the radiation, which passed through only one zone.EFFECT: device makes it possible to carry out the qualitative and quantitative analysis of the nucleotide sequences, increases the accuracy of the identification of the nucleotide sequences.3 cl, 5 ex Изобретение относится к области молекулярной биологии и биохимии. Устройство состоит из источника света, излучение от которого направлено на прозрачную подложку с иммобилизованными на ее поверхности олигонуклеотидами и расположенной под ней системой детекции интенсивности света, прошедшего через подложку. Подложка содержит не менее двух зон, на поверхности одной из которых иммобилизован слой олигонуклеотидов, неспецифических к исследуемой последовательности нуклеотидов, а на поверхности другой зоны иммобилизован слой олигонуклеотидов, специфических к исследуемой последовательности нуклеотидов. Причем система детекции содержит не менее двух фоточувствительных независимых секций, каждая из которых освещена излучением, прошедшим только через одну зону. Устройство позволяет проводить качественный и количественный анализ последовательностей нуклеотидов, повышает точность идентификации последовательностей нуклеотидов. 2 з.п. ф-лы, 5 пр.
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A device consists of a light source, the radiation of which is directed on a transparent substrate with oligonucleotides immobilised on its surface and a system of detecting the intensity of light, which passed through the substrate, located under it. The substrate contains at least two zones, with a layer of oligonucleotides, non-specific to the nucleotide sequence under study, being immobilised on the surface of one of them, and a layer of nucleotides, specific to the nucleotide sequence under study, being immobilised on the surface of the other zone. The detection system contains at least two photosensitive independent sections, each of which is illuminated by the radiation, which passed through only one zone.EFFECT: device makes it possible to carry out the qualitative and quantitative analysis of the nucleotide sequences, increases the accuracy of the identification of the nucleotide sequences.3 cl, 5 ex Изобретение относится к области молекулярной биологии и биохимии. Устройство состоит из источника света, излучение от которого направлено на прозрачную подложку с иммобилизованными на ее поверхности олигонуклеотидами и расположенной под ней системой детекции интенсивности света, прошедшего через подложку. Подложка содержит не менее двух зон, на поверхности одной из которых иммобилизован слой олигонуклеотидов, неспецифических к исследуемой последовательности нуклеотидов, а на поверхности другой зоны иммобилизован слой олигонуклеотидов, специфических к исследуемой последовательности нуклеотидов. Причем система детекции содержит не менее двух фоточувствительных независимых секций, каждая из которых освещена излучением, прошедшим только через одну зону. 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Устройство состоит из источника света, излучение от которого направлено на прозрачную подложку с иммобилизованными на ее поверхности олигонуклеотидами и расположенной под ней системой детекции интенсивности света, прошедшего через подложку. Подложка содержит не менее двух зон, на поверхности одной из которых иммобилизован слой олигонуклеотидов, неспецифических к исследуемой последовательности нуклеотидов, а на поверхности другой зоны иммобилизован слой олигонуклеотидов, специфических к исследуемой последовательности нуклеотидов. Причем система детекции содержит не менее двух фоточувствительных независимых секций, каждая из которых освещена излучением, прошедшим только через одну зону. 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A device consists of a light source, the radiation of which is directed on a transparent substrate with oligonucleotides immobilised on its surface and a system of detecting the intensity of light, which passed through the substrate, located under it. The substrate contains at least two zones, with a layer of oligonucleotides, non-specific to the nucleotide sequence under study, being immobilised on the surface of one of them, and a layer of nucleotides, specific to the nucleotide sequence under study, being immobilised on the surface of the other zone. The detection system contains at least two photosensitive independent sections, each of which is illuminated by the radiation, which passed through only one zone.EFFECT: device makes it possible to carry out the qualitative and quantitative analysis of the nucleotide sequences, increases the accuracy of the identification of the nucleotide sequences.3 cl, 5 ex Изобретение относится к области молекулярной биологии и биохимии. Устройство состоит из источника света, излучение от которого направлено на прозрачную подложку с иммобилизованными на ее поверхности олигонуклеотидами и расположенной под ней системой детекции интенсивности света, прошедшего через подложку. Подложка содержит не менее двух зон, на поверхности одной из которых иммобилизован слой олигонуклеотидов, неспецифических к исследуемой последовательности нуклеотидов, а на поверхности другой зоны иммобилизован слой олигонуклеотидов, специфических к исследуемой последовательности нуклеотидов. Причем система детекции содержит не менее двух фоточувствительных независимых секций, каждая из которых освещена излучением, прошедшим только через одну зону. Устройство позволяет проводить качественный и количественный анализ последовательностей нуклеотидов, повышает точность идентификации последовательностей нуклеотидов. 2 з.п. ф-лы, 5 пр.</abstract><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
title DEVICE FOR IDENTIFICATION OF NUCLEOTIDE SEQUENCES
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