SINGLE-DOMAIN MINI-ANTIBODY aHER2/aSKBR3-1, SPECIFICALLY BINDING EPIDERMAL GROWTH FACTOR RECEPTOR HER2/ERBB2/neu AND CAPABLE TO PENETRATE THROUGH THIS INTERACTION INTO TARGET CELLS (INTERNALISED), METHOD OF PRODUCTION OF THIS ANTIBODY AND METHOD OF DETECTION OF PROTEIN HER2/ERBB2/neu AND CELLS EXPRESSING THIS PROTEIN IN INCREASED AMOUNT, USING MINI-ANTIBODY aHER2/aSKBR3-1

SINGLE-DOMAIN MINI-ANTIBODY aHER2/aSKBR3-1, SPECIFICALLY BINDING EPIDERMAL GROWTH FACTOR RECEPTOR HER2/ERBB2/neu AND CAPABLE TO PENETRATE THROUGH THIS INTERACTION INTO TARGET CELLS (INTERNALISED), METHOD OF PRODUCTION OF THIS ANTIBODY AND METHOD OF DETECTION OF PROTEIN HER2/ERBB2/neu AND CELLS EXPRESSING THIS PROTEIN IN INCREASED AMOUNT, USING MINI-ANTIBODY aHER2/aSKBR3-1

FIELD: biotechnology.SUBSTANCE: single-domain mini-antibody is proposed, specifically binding the protein-receptor of epidermal growth factor of human HER2/ERBB2/neu obtained by immunisation of two-humped camel (Camelus bactrianus) by the preparation of tumour cells SKBR3, and characterised by the a...

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Hauptverfasser: VASIL'EV LEV ANATOL'EVICH, ZAGAJNOVA ELENA VADIMOVNA, IVANOVA TAT'JANA IL'INICHNA, TILLIB SERGEJ VLADIMIROVICH, DEEV SERGEJ MIKHAJLOVICH, ZDOBNOVA TAT'JANA ALEKSANDROVNA
Format: Patent
Sprache:eng ; rus
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CHEMISTRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
TESTING
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Zusammenfassung:FIELD: biotechnology.SUBSTANCE: single-domain mini-antibody is proposed, specifically binding the protein-receptor of epidermal growth factor of human HER2/ERBB2/neu obtained by immunisation of two-humped camel (Camelus bactrianus) by the preparation of tumour cells SKBR3, and characterised by the amino acid sequence. Also the method of detecting protein HER2/ERBB2/neu and its expressing cells is considered. The antibody according to the present invention is able to penetrate in the target cell on which surface HER2/ERBB2/neu is exposed, and may find further application in diagnostics and therapy of diseases associated with overexpression of HER2/ERBB2/neu.EFFECT: improving efficiency of use of the compound.2 cl, 6 dwg, 5 ex Настоящее изобретение относится к области иммунологии. Предложено однодоменное мини-антитело, специфически связывающее белок-рецептор эпидермального фактора роста HER2/ERBB2/neu человека, полученное при иммунизации двугорбого верблюда (Camelus bactrianus) препаратом опухолевых клеток SKBR3, и охарактеризованное аминокислотной последовательностью. Также рассмотрен способ детекции белка HER2/ERBB2/neu и экспрессирующих его клеток. Антитело по настоящему изобретению способно проникать внутрь клетки-мишени, на поверхности которой экспонирован HER2/ERBB2/neu, и может найти дальнейшее применение в диагностике и терапии заболеваний, связанных с повышенной экспрессией HER2/ERBB2/neu. 2 н.п. ф-лы, 6 ил., 5 пр.