RECOMBINANT PLASMID, RECOMBINANT STRAIN, RECOMBINANT PROTEIN BMP-7 AND EXTRACTION METHOD OF RECOMBINANT PROTEIN IN DIMERIC FORM

FIELD: biotechnologies.SUBSTANCE: invention represents recombinant BMPRIB-CBD plasmid, and E.coli strain transformed with that plasmid. The invention also refers to recombinant BMPRIB-CBD protein that is used for production of BMP-7 protein.EFFECT: invention allows producing chromatographic clean fr...

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Hauptverfasser: SERGIENKO OL'GA VASIL'EVNA, GROMOV ALEKSANDR VIKTOROVICH, KARJAGINA-ZHULINA ANNA STANISLAVOVNA, LJASHCHUK ALEKSANDR MIKHAJLOVICH, GALUSHKINA ZOJA MIKHAJLOVNA, SEMIKHIN ALEKSANDR SERGEEVICH, KOTNOVA ALINA PETROVNA, KARPOVA TAT'JANA ANDREEVNA, ERSHOVA ANNA STEPANOVNA, SUBBOTINA MARINA EVGEN'EVNA, POLETAEVA NINA NIKOLAEVNA, SHARAPOVA NATAL'JA EVGEN'EVNA, LAVROVA NATAL'JA VITAL'EVNA, GINTSBURG ALEKSANDR LEONIDOVICH, GRUNINA TAT'JANA MIKHAJLOVNA, BARTOV MIKHAIL SERGEEVICH, LUNIN VLADIMIR GLEBOVICH
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creator SERGIENKO OL'GA VASIL'EVNA
GROMOV ALEKSANDR VIKTOROVICH
KARJAGINA-ZHULINA ANNA STANISLAVOVNA
LJASHCHUK ALEKSANDR MIKHAJLOVICH
GALUSHKINA ZOJA MIKHAJLOVNA
SEMIKHIN ALEKSANDR SERGEEVICH
KOTNOVA ALINA PETROVNA
KARPOVA TAT'JANA ANDREEVNA
ERSHOVA ANNA STEPANOVNA
SUBBOTINA MARINA EVGEN'EVNA
POLETAEVA NINA NIKOLAEVNA
SHARAPOVA NATAL'JA EVGEN'EVNA
LAVROVA NATAL'JA VITAL'EVNA
GINTSBURG ALEKSANDR LEONIDOVICH
GRUNINA TAT'JANA MIKHAJLOVNA
BARTOV MIKHAIL SERGEEVICH
LUNIN VLADIMIR GLEBOVICH
description FIELD: biotechnologies.SUBSTANCE: invention represents recombinant BMPRIB-CBD plasmid, and E.coli strain transformed with that plasmid. The invention also refers to recombinant BMPRIB-CBD protein that is used for production of BMP-7 protein.EFFECT: invention allows producing chromatographic clean fractions of biologically active dimeric form of BMP-7 protein, which can be used as osteoinductive components of osteoplastic materials of new generation.4 cl, 2 dwg, 6 ex Изобретение относится к биотехнологии и представляет собой рекомбинантную плазмиду BMPRIB-CBD, штамм E.coli, трансформированный данной плазмидой. Изобретение относится также к рекомбинантному белку BMPRIB-CBD, с использованием которого получают белок BMP-7. Изобретение позволяет получить хроматографически чистые фракции биологически активной димерной формы белка BMP-7, которые могут быть использованы в качестве остеоиндуктивных компонентов костнопластических материалов нового поколения. 4 н.п. ф-лы, 2 ил., 6 пр.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title RECOMBINANT PLASMID, RECOMBINANT STRAIN, RECOMBINANT PROTEIN BMP-7 AND EXTRACTION METHOD OF RECOMBINANT PROTEIN IN DIMERIC FORM
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T14%3A32%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=SERGIENKO%20OL'GA%20VASIL'EVNA&rft.date=2013-11-20&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ERU2499047C1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true