METHOD FOR NONCOVALENT IMMOBILISATION OF LIPOPOLYSACCHARIDE Shigella flexneri 2a ON SOLID HYDROPHOBIC CARRIER
FIELD: chemistry. ^ SUBSTANCE: method involves passing a solution of lipopolysaccharide Shigella flexneri 2a in a 0.05 M triethylammonium acetate buffer at pH 4.7, which contains 10% n-propanol, through a column with a hydrophobic carrier which is Octyl Sepharose CL-4B, and then washing the column w...
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| creator | VANEEVA NATAL'JA PAVLOVNA SHEKHT MARIJA EVGEN'EVNA NOVIKOV VLADIMIR IVANOVICH SURKOVA INNA GENRIKHOVNA GOLOVINA MARINA EHDUARDOVNA L'VOV VJACHESLAV LEONIDOVICH ELKINA STANISLAVA IVANOVNA VERNER IRINA KARLOVNA JASTREBOVA NATAL'JA EVGEN'EVNA APARIN PETR GENNAD'EVICH |
| description | FIELD: chemistry. ^ SUBSTANCE: method involves passing a solution of lipopolysaccharide Shigella flexneri 2a in a 0.05 M triethylammonium acetate buffer at pH 4.7, which contains 10% n-propanol, through a column with a hydrophobic carrier which is Octyl Sepharose CL-4B, and then washing the column with the same buffer solution. ^ EFFECT: method can be used to produce carriers which enable extraction and purification of specific antibodies to lipopolysaccharides using affinity chromatography. ^ 1 ex
Изобретение относится к области иммунофармакологии и касается способа иммобилизации липополисахарида Shigella flexneri 2a на твердом гидрофобном носителе. Представленный способ включает проведение через колонку с гидрофобным носителем, представляющим собой октил-Сефарозу CL-4B, раствора липополисахарида Shigella flexneri 2a в 0,05 М триэтиламмонийацетатном буфере, рН 4,7, содержащем 10% н-пропанола, с последующей отмывкой колонки тем же буфером. Описанный способ может быть использован для получения носителей, позволяющих проводить выделение и очистку специфических антител к липополисахаридам с помощью аффинной хроматографии. 1 пр. |
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Изобретение относится к области иммунофармакологии и касается способа иммобилизации липополисахарида Shigella flexneri 2a на твердом гидрофобном носителе. Представленный способ включает проведение через колонку с гидрофобным носителем, представляющим собой октил-Сефарозу CL-4B, раствора липополисахарида Shigella flexneri 2a в 0,05 М триэтиламмонийацетатном буфере, рН 4,7, содержащем 10% н-пропанола, с последующей отмывкой колонки тем же буфером. Описанный способ может быть использован для получения носителей, позволяющих проводить выделение и очистку специфических антител к липополисахаридам с помощью аффинной хроматографии. 1 пр.</description><language>eng ; rus</language><subject>ACYCLIC OR CARBOCYCLIC COMPOUNDS ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; SEPARATION ; SPIRITS ; TRANSPORTING ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120410&DB=EPODOC&CC=RU&NR=2447153C1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120410&DB=EPODOC&CC=RU&NR=2447153C1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>VANEEVA NATAL'JA PAVLOVNA</creatorcontrib><creatorcontrib>SHEKHT MARIJA EVGEN'EVNA</creatorcontrib><creatorcontrib>NOVIKOV VLADIMIR IVANOVICH</creatorcontrib><creatorcontrib>SURKOVA INNA GENRIKHOVNA</creatorcontrib><creatorcontrib>GOLOVINA MARINA EHDUARDOVNA</creatorcontrib><creatorcontrib>L'VOV VJACHESLAV LEONIDOVICH</creatorcontrib><creatorcontrib>ELKINA STANISLAVA IVANOVNA</creatorcontrib><creatorcontrib>VERNER IRINA KARLOVNA</creatorcontrib><creatorcontrib>JASTREBOVA NATAL'JA EVGEN'EVNA</creatorcontrib><creatorcontrib>APARIN PETR GENNAD'EVICH</creatorcontrib><title>METHOD FOR NONCOVALENT IMMOBILISATION OF LIPOPOLYSACCHARIDE Shigella flexneri 2a ON SOLID HYDROPHOBIC CARRIER</title><description>FIELD: chemistry. ^ SUBSTANCE: method involves passing a solution of lipopolysaccharide Shigella flexneri 2a in a 0.05 M triethylammonium acetate buffer at pH 4.7, which contains 10% n-propanol, through a column with a hydrophobic carrier which is Octyl Sepharose CL-4B, and then washing the column with the same buffer solution. ^ EFFECT: method can be used to produce carriers which enable extraction and purification of specific antibodies to lipopolysaccharides using affinity chromatography. ^ 1 ex
Изобретение относится к области иммунофармакологии и касается способа иммобилизации липополисахарида Shigella flexneri 2a на твердом гидрофобном носителе. Представленный способ включает проведение через колонку с гидрофобным носителем, представляющим собой октил-Сефарозу CL-4B, раствора липополисахарида Shigella flexneri 2a в 0,05 М триэтиламмонийацетатном буфере, рН 4,7, содержащем 10% н-пропанола, с последующей отмывкой колонки тем же буфером. Описанный способ может быть использован для получения носителей, позволяющих проводить выделение и очистку специфических антител к липополисахаридам с помощью аффинной хроматографии. 1 пр.</description><subject>ACYCLIC OR CARBOCYCLIC COMPOUNDS</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PERFORMING OPERATIONS</subject><subject>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</subject><subject>SEPARATION</subject><subject>SPIRITS</subject><subject>TRANSPORTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2012</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNzL0KwjAUQOEuDqK-w30Bh_6Ic0xSciHNLUkVOpUgqRZiLdbBx7eDD-B0lo-zTh6VbBQJKMmCIcPpwrQ0DWBV0Qk1OtYgGaASNNZUk24d41wxi0KCuw-3EKOHPobPGF4DZB4W7UijANUKS7VaNhw4sxal3Sar3sc57H7dJFDKhqt9mJ5dmCd_DWN4d_acFcUxPeQ8zf8gX-ymOO8</recordid><startdate>20120410</startdate><enddate>20120410</enddate><creator>VANEEVA NATAL'JA PAVLOVNA</creator><creator>SHEKHT MARIJA EVGEN'EVNA</creator><creator>NOVIKOV VLADIMIR IVANOVICH</creator><creator>SURKOVA INNA GENRIKHOVNA</creator><creator>GOLOVINA MARINA EHDUARDOVNA</creator><creator>L'VOV VJACHESLAV LEONIDOVICH</creator><creator>ELKINA STANISLAVA IVANOVNA</creator><creator>VERNER IRINA KARLOVNA</creator><creator>JASTREBOVA NATAL'JA EVGEN'EVNA</creator><creator>APARIN PETR GENNAD'EVICH</creator><scope>EVB</scope></search><sort><creationdate>20120410</creationdate><title>METHOD FOR NONCOVALENT IMMOBILISATION OF LIPOPOLYSACCHARIDE Shigella flexneri 2a ON SOLID HYDROPHOBIC CARRIER</title><author>VANEEVA NATAL'JA PAVLOVNA ; SHEKHT MARIJA EVGEN'EVNA ; NOVIKOV VLADIMIR IVANOVICH ; SURKOVA INNA GENRIKHOVNA ; GOLOVINA MARINA EHDUARDOVNA ; L'VOV VJACHESLAV LEONIDOVICH ; ELKINA STANISLAVA IVANOVNA ; VERNER IRINA KARLOVNA ; JASTREBOVA NATAL'JA EVGEN'EVNA ; APARIN PETR GENNAD'EVICH</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_RU2447153C13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rus</language><creationdate>2012</creationdate><topic>ACYCLIC OR CARBOCYCLIC COMPOUNDS</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PERFORMING OPERATIONS</topic><topic>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</topic><topic>SEPARATION</topic><topic>SPIRITS</topic><topic>TRANSPORTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>VANEEVA NATAL'JA PAVLOVNA</creatorcontrib><creatorcontrib>SHEKHT MARIJA EVGEN'EVNA</creatorcontrib><creatorcontrib>NOVIKOV VLADIMIR IVANOVICH</creatorcontrib><creatorcontrib>SURKOVA INNA GENRIKHOVNA</creatorcontrib><creatorcontrib>GOLOVINA MARINA EHDUARDOVNA</creatorcontrib><creatorcontrib>L'VOV VJACHESLAV LEONIDOVICH</creatorcontrib><creatorcontrib>ELKINA STANISLAVA IVANOVNA</creatorcontrib><creatorcontrib>VERNER IRINA KARLOVNA</creatorcontrib><creatorcontrib>JASTREBOVA NATAL'JA EVGEN'EVNA</creatorcontrib><creatorcontrib>APARIN PETR GENNAD'EVICH</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>VANEEVA NATAL'JA PAVLOVNA</au><au>SHEKHT MARIJA EVGEN'EVNA</au><au>NOVIKOV VLADIMIR IVANOVICH</au><au>SURKOVA INNA GENRIKHOVNA</au><au>GOLOVINA MARINA EHDUARDOVNA</au><au>L'VOV VJACHESLAV LEONIDOVICH</au><au>ELKINA STANISLAVA IVANOVNA</au><au>VERNER IRINA KARLOVNA</au><au>JASTREBOVA NATAL'JA EVGEN'EVNA</au><au>APARIN PETR GENNAD'EVICH</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR NONCOVALENT IMMOBILISATION OF LIPOPOLYSACCHARIDE Shigella flexneri 2a ON SOLID HYDROPHOBIC CARRIER</title><date>2012-04-10</date><risdate>2012</risdate><abstract>FIELD: chemistry. ^ SUBSTANCE: method involves passing a solution of lipopolysaccharide Shigella flexneri 2a in a 0.05 M triethylammonium acetate buffer at pH 4.7, which contains 10% n-propanol, through a column with a hydrophobic carrier which is Octyl Sepharose CL-4B, and then washing the column with the same buffer solution. ^ EFFECT: method can be used to produce carriers which enable extraction and purification of specific antibodies to lipopolysaccharides using affinity chromatography. ^ 1 ex
Изобретение относится к области иммунофармакологии и касается способа иммобилизации липополисахарида Shigella flexneri 2a на твердом гидрофобном носителе. Представленный способ включает проведение через колонку с гидрофобным носителем, представляющим собой октил-Сефарозу CL-4B, раствора липополисахарида Shigella flexneri 2a в 0,05 М триэтиламмонийацетатном буфере, рН 4,7, содержащем 10% н-пропанола, с последующей отмывкой колонки тем же буфером. Описанный способ может быть использован для получения носителей, позволяющих проводить выделение и очистку специфических антител к липополисахаридам с помощью аффинной хроматографии. 1 пр.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ACYCLIC OR CARBOCYCLIC COMPOUNDS BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL SEPARATION SPIRITS TRANSPORTING VINEGAR WINE |
| title | METHOD FOR NONCOVALENT IMMOBILISATION OF LIPOPOLYSACCHARIDE Shigella flexneri 2a ON SOLID HYDROPHOBIC CARRIER |
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