METHOD OF SIMULATING BUILD-UP OF BACTERIAL RESISTANCE TO DISINFECTANT

FIELD: chemistry. ^ SUBSTANCE: method involves preliminary selection of concentration of a disinfectant solution at which bacteria exhibit partial sensitivity to said disinfectant. The bacteria are exposed to the disinfectant solution at the given concentration. Grown colonies of bacteria are collected after exposure to said disinfectant and then sown on a solid culture medium, followed by culturing at 37C. Bacteria colonies isolated from those grown at the previous step are selected in amount of 1-299 colony-forming units (CFU/ml). The collected isolated colonies are resown on a culture medium and grown at conditions needed for growth of bacteria of that type, followed by dividing the grown colonies into two parts. One part of the grown bacteria colonies is exposed to the disinfectant at the selected concentration, and the other part is exposed to the disinfectant at bactericidal concentration. If upon exposure to the disinfectant at bactericidal concentration, there is no growth of bacteria or their growth ranges from 1 to 299 (CFU/ml), then the cycle of selecting bacteria grown at the previous step, their resowing on the culture medium, growing colonies of said bacteria at conditions needed for growth of said type of bacteria and exposing the grown colonies to the disinfectant at the selected and bactericidal concentrations is repeated once more until after exposing the column to the disinfectant solution at bactericidal concentration, growth of bacteria on the culture medium is equal to 300 (CFU/ml), which indicates bacterial resistance to the disinfectant. ^ EFFECT: invention enables to simulate bacterial resistance to a disinfectant, evaluate build-up of resistance and bactericidal potential of hospital strains to disinfectants during their practical use. ^ 1 tbl, 1 ex Изобретение относится к биотехнологии и может быть использовано для моделирования формирования устойчивости бактерий к дезинфицирующему средству и для оценки бактерицидного действия дезинфицирующих средств. Способ предусматривает предварительный подбор концентрации раствора дезинфицирующего средства, при которой бактерии проявляют неполную чувствительность к данному средству. Воздействие на бактерии раствором дезинфицирующего средства при данной концентрации. Отбор выросших колоний бактерий после воздействия дезинфицирующего средства и высев их на твердую питательную среду с последующим культивированием при температуре 37°С. Отбор изолированных колоний бактерий из выросших на предыдуще