RECOMBINANT PLASMID DNA, ENCODING HUMAN FACTOR VII PROTEIN SEQUENCE, CELL LINE BHK/F7, TRANSFORMED WITH PLASMID DNA

FIELD: chemistry, biotechnology. ^ SUBSTANCE: invention relates to field of biotechnology and concerns obtaining factor VII protein by method of recombinant DNA. Recombinant plasmid DNA was constructed for expression of blood clotting factor VII in mammalian cells, which is product of ligating of fr...

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Hauptverfasser:	VOROB'EV IVAN IVANOVICH, MIROSHNIKOV ANATOLIJ IVANOVICH, GABIBOV ALEKSANDR GABIBOVICH, SHAMBORANT OL'GA GEORGIEVNA, MART'JANOV VITALIJ AFANAS'EVICH, VOROB'EV ANDREJ IVANOVICH, SHUSTER ALEKSANDR MIKHAJLOVICH, PONOMARENKO NATAL'JA ALEKSANDROVNA
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	FIELD: chemistry, biotechnology. ^ SUBSTANCE: invention relates to field of biotechnology and concerns obtaining factor VII protein by method of recombinant DNA. Recombinant plasmid DNA was constructed for expression of blood clotting factor VII in mammalian cells, which is product of ligating of fragment of cDNA of human factor VII gene, flanked by sites of restrictases Xhol and BamHI recognising, with large XhoI/BglII fragment of vector pEFZeo, including genes of resistance to ampicillin and zeocin. As result of BHK cell transformation with new recombinant plasmid, cell line BHK/F7 was obtained, which produces recombinant protein of factor VII with output of up to 40 mkg/ml. ^ EFFECT: obtaining cell line producing recombinant protein of factor VII. ^ 2 cl, 4 dwg, 4 ex