METHOD AND BIOCHIP FOR RECORDING MACROMOLECULES IN CARRYING OUT PROTEOMIC RESEARCH
FIELD: medicine; medical engineering. ^ SUBSTANCE: method involves recording macromolecules and their complexes by applying scanning probe microscopy approach and a biochip representing immobilized molecule field capable of interaction in covalent or non-covalent way to the macromolecules under stud...
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| creator | IVANOV JURIJ DMITRIEVICH ZIBOROV VADIM SERAFIMOVICH ARCHAKOV ALEKSANDR IVANOVICH GOVORUN VADIM ALEKSANDROVICH KIRPICHNIKOV MIKHAIL PETROVICH SVETLOV SERGEJ KONSTANTINOVICH POZDNEEV VLADIMIR FEDOROVICH BYKOV VIKTOR ALEKSANDROVICH |
| description | FIELD: medicine; medical engineering. ^ SUBSTANCE: method involves recording macromolecules and their complexes by applying scanning probe microscopy approach and a biochip representing immobilized molecule field capable of interaction in covalent or non-covalent way to the macromolecules under study and/or their complexes on mica substrate having surface treated with electric discharge plasma by applying constant or low frequency voltage to discharge gap between sample and chemically modified Sylane. Biochip is placed into solution containing macromolecules under study and/or their complexes. The macromolecules under study and/or their complexes are caught due to covalent or non-covalent interaction with molecules immobilized on substrate and recorded after having washed the field from nonspecific complexes. ^ EFFECT: high sensitivity of the method. ^ 4 cl, 3 dwg |
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Biochip is placed into solution containing macromolecules under study and/or their complexes. The macromolecules under study and/or their complexes are caught due to covalent or non-covalent interaction with molecules immobilized on substrate and recorded after having washed the field from nonspecific complexes. ^ EFFECT: high sensitivity of the method. ^ 4 cl, 3 dwg</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBEMICROSCOPY [SPM] INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS SCANNING-PROBE TECHNIQUES OR APPARATUS TESTING |
| title | METHOD AND BIOCHIP FOR RECORDING MACROMOLECULES IN CARRYING OUT PROTEOMIC RESEARCH |
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