METHOD FOR PREPARING PREPARATION DRY ALPHA-FETOPROTEIN

FIELD: proteins, chemical-pharmaceutical industry. ^ SUBSTANCE: dry alpha-fetoprotein (AFP) is isolated from blood serum. Method involves fractionation of proteins with ethyl alcohol of ammonium sulfate, or sodium sulfate. After separation of supernatant the latter is dialyzed against sodium chloride solution and applied on affinity sorbent in sodium chloride solution with the concentration 0.1-1.0 mole/l and Triton X-100 with the concentration 0.01-0.5%. Sorbent is washed out with phosphate-saline buffer and AFP bound with sorbent is eluted by using glycine-HCl buffer solution. Eluate is neutralized and applied on Sepharose with antibodies raised to human IgG used as the second affinity sorbent followed by sterilizing filtration and lyophilization. Invention provides enhancing yield of AFP and its purity. ^ EFFECT: improved preparing method. ^ 4 ex