METHOD FOR OBTAINING COLLAGEN FROM BONE TISSUE
FIELD: medicine. ^ SUBSTANCE: method involves sawing bone into 0.1-2.0 cm thick plates, washing them with 0.1 M phosphate buffer solution having pH equal to 5.8-6.0. The tissue is digested in activated 0.125-0.325% papain at 60°C during 24 h. The plates are washed with 5 water volumes at 60°C and tr...
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