METHOD FOR DETECTING FUNCTIONAL ACTIVITY OF SUBCOMPONENTS C1r2s2 OF THE FIRST COMPONENT OF HUMAN COMPLEMENT

FIELD: medicinal immunology. ^ SUBSTANCE: one should sorb inspecific immunoglobulin G in microplot's holes, then one should successively supplement these holes with solution containing guinea-pig's serum and sodium ethylenediaminotetraacetate and after incubation - an analyzed sample containing Clr2s2 complex of human component at unknown activity and pseudoglobulin fraction of human blood serum obtained due to dialysis against distilled water, and, also, a buffer solution that contains calcium and magnesium ions and after incubation it is necessary to wash and dry the plot, holes should be supplemented with enzymatic conjugate with antibodies against human C3 component and substrate of this enzyme, according to the quantity of formed product of enzymatic reaction one should calculate the activity of Clr2s2 complex. ^ EFFECT: higher efficiency of detection. ^ 1 ex, 1 tbl