METHOD OF CASCADE-FLOWING MICROORGANISMS CULTURING

FIELD: microbiology. SUBSTANCE: invention relates to method of culturing microorganisms in fermentation devices in liquid nutrient media. Method involves batch feeding nutrient medium in the first fermenter after pouring off excessive volume cultural fluid. Cultural fluid in cascade flow is transfer...

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Hauptverfasser: KUDRJASHOV V.K, SHIRSHIKOV N.V, REDIKUL'TSEV JU.V, PERMJAKOV E.A
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creator KUDRJASHOV V.K
SHIRSHIKOV N.V
REDIKUL'TSEV JU.V
PERMJAKOV E.A
description FIELD: microbiology. SUBSTANCE: invention relates to method of culturing microorganisms in fermentation devices in liquid nutrient media. Method involves batch feeding nutrient medium in the first fermenter after pouring off excessive volume cultural fluid. Cultural fluid in cascade flow is transferred from the first fermenter to the next devices with given interval being in all next fermenters cultural fluid is replaced by cultural fluid volume from the previous fermenter. Operation by cascade flow is performed by time set-up of culturing and pause between cycles of cascade flow. Method ensures to separate growth phases of microorganisms by time and in the space. Invention can be used in production of bacterial synthesis and research practice. EFFECT: improved method of culturing. 1 dwg, 2 ex
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SUBSTANCE: invention relates to method of culturing microorganisms in fermentation devices in liquid nutrient media. Method involves batch feeding nutrient medium in the first fermenter after pouring off excessive volume cultural fluid. Cultural fluid in cascade flow is transferred from the first fermenter to the next devices with given interval being in all next fermenters cultural fluid is replaced by cultural fluid volume from the previous fermenter. Operation by cascade flow is performed by time set-up of culturing and pause between cycles of cascade flow. Method ensures to separate growth phases of microorganisms by time and in the space. Invention can be used in production of bacterial synthesis and research practice. 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SUBSTANCE: invention relates to method of culturing microorganisms in fermentation devices in liquid nutrient media. Method involves batch feeding nutrient medium in the first fermenter after pouring off excessive volume cultural fluid. Cultural fluid in cascade flow is transferred from the first fermenter to the next devices with given interval being in all next fermenters cultural fluid is replaced by cultural fluid volume from the previous fermenter. Operation by cascade flow is performed by time set-up of culturing and pause between cycles of cascade flow. Method ensures to separate growth phases of microorganisms by time and in the space. Invention can be used in production of bacterial synthesis and research practice. EFFECT: improved method of culturing. 1 dwg, 2 ex</abstract><edition>7</edition><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title METHOD OF CASCADE-FLOWING MICROORGANISMS CULTURING
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